Manali Nagarhalli

Rockville, MD ***** 412-***-**** add5gd@r.postjobfree.com

linkedin.com/in/manalinagarhalli medium.com/@manalipn97

EDUCATION

University of Maryland Baltimore Rockville, Maryland

Master of Science, Pharmaceutical Sciences (GPA 3.75/4) August 2019-December 2020 (Expected)

Bharati Vidyapeeth University Pune, India

Bachelor of Pharmacy (CGPA 9.11/10) June 2015-May 2019

SKILLS & TECHNIQUES

Cell Culture: Cryopreservation, MTT assay

Microbial QC Testing method: LAL testing, Microbial limit test (MLT)

Molecular Biology method: PCR, DNA Cloning

Analytical Techniques: NMR spectroscopy, UV spectroscopy

Data Retrieval tools: NCBI

Sequence Alignment tools: BLAST

Threading Algorithm: RaptorX

Structure prediction tools: Expasy, Uniprot, JPred, Swiss model

Validation programs: VADAR

Microsoft Excel- Statistical tests: ANOVA, Linear regression

Chemical Databases and software: PyMOL, SciFinder, SOLO

COURSES

●Principles of Drug Discovery

●Principles of Drug Development

●Bioanalytical and Pharmacological Methods

●Ethics and Biostatistics

●Spectrometric Methods

●Process Analytical Technologies for Pharmaceutical Manufacturing

●Metallobiochemistry

●Research Project Design and Management

●Scientific Writing

PROJECTS

Bioinformatics Fall 2019

●Determined protein data based on a DNA sequence using Bioinformatics tools.

●Analysed protein sequence using BLASTX and amino acid sequence using BLAST.

●Predicted protein class and family, secondary structure and post translational modifications using Uniprot.

●Determined Membrane topology and tertiary structure of protein using TMHMM and Swiss model respectively.

●Used VADAR and JPred for validation of structure.

●Analysed structure of the initial and final 40 amino acids of the protein using Threading Algorithm RaptorX.

Cloning and CD2 Expression Fall 2019

●Devised a strategy to clone and express CD2 gene into a vector.

●Selected pGEX-3X with GST tag as a vector and used BamHI and EcoRI as restriction enzymes.

●Designed a primer for CD2 gene.

●Performed PCR and transformation of vector into E. coli.

●Carried out cell lysis using BugBuster Extraction Reagent.

●Purified and verified the protein using affinity chromatography and SDS.

Biosensors: Types and Applications Spring 2019

Performed Literature review on Biosensors and their types construction and working of various biosensors, applications in the field of medicine with major focus on biosensors for Breast Cancer.

INTERNSHIP

Summer Virtual Internship

GEn1E Lifesciences, Palo Alto, California Present

Regulatory Science in Drug Development

Research Intern

Institute for Bioscience and Biotechnology Research, Rockville, Maryland Jan 2020

Analysed effects of nanoparticles on BSA and Human IgGs using water proton NMR.

●Prepared 1X and 2X PBS buffer, BSA and Y-globulin solutions and maintained at pH 7.4.

●Prepared dilutions of buffer and proteins with nanoparticles.

●Measured water proton transverse relaxation rates using low-field benchtop NMR instrument.

●Performed calculations and analysed the data using Microsoft Excel.

Intern

Serum Institute of India, Pune, India June 2018

●Trained in Bacterial Vaccination, Viral Vaccination and Quality Control Departments.

●Familiarized with Good Manufacturing Practices (GMP) and Good Lab Practices (GLP).

●Analysed vaccines biochemically, Environmental Monitoring, raw material, in process and finished product testing of vaccines.

●Performed Microbial limit test and Limulus Amoebocyte Lysate test.

CERTIFICATIONS

Chest Research Foundation, Pune, India April 2018

●Trained in statistical techniques and research methodology.

●Familiarized with various sampling techniques.

●Performed statistical tests like t-test, ANOVA and chi-square test.

Chest Research Foundation, Pune, India October 2017

●Trained in Cell culture techniques and methodology.

●Worked with RAW 264.7 cells and studied the cytotoxic effect of toxins on the cells and calculated cell death.

●Carried out sub-culturing and cell counting, cryopreservation and revival of cells.

●Performed MTT assay.

EXTRA-CURRICULAR

National Service Scheme – Volunteer July 2018

●Carried out patient counselling related to appropriate use of medication

●Conducted health check-ups.

Indian Pharmaceutical Association Students Forum - Delegate March 2016

●Delegate at Pharma Eidolon-II, Educational Symposium on therapeutic applications of curcumin for treating pancreatic cancer.

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