RIYA SHAH
*********@*******.***.*** www.linkedin.com/in/riyashah5 +1-774-***-****
Lowell, Massachusetts
EDUCATION
Master of Science in Biological Science (Biotechnology)-GPA: 3.7/4.0 Anticipated May 2020 University of Massachusetts Lowell-Lowell, MA
Coursework: Molecular Techniques, Cancer Biology, Immunology, Recombinant Protein Production Techniques and Biochemistry.
Master of Science in Biochemistry May 2017
Maharaja Sayajirao University, India
Bachelor of Science in Biotechnology April 2015
Sardar Patel University, India
Awarded gold medal for earning top grades in University TECHNICAL SKILLS
Laboratory: Transduction and Transformation, Cell culture (Mammalian and insect cells) Column chromatography (IEC, SEC, Affinity), AKTA (FPLC), SDS PAGE, Western blotting, PCR, Clone generation, ELISA, DNA and RNA isolation, Immunoassays and protein purification assays.
Computer: BLAST, SAP, MS Office (Excel, Word, PowerPoint) ACADEMIC PROJECTS
Master’s Project- August 2019-Present
Purification of ERAP2 using FPLC (Endoplasmic Reticulum Aminopeptidase II)
Generated Expression clone for ERAP2 using Gateway Recombination Cloning
Transformation of Expression clone in to DH10Bac competent cells and Bacmid isolation
Cell culture of SF9 insect cells
Expression of ERAP2 into Baculovirus expression system by transfecting Bacmid DNA into SF9 insect cells Master’s Thesis – June 2016-April 2017
Evidences for the overproduction of ppGpp in rumA::CAT mutant and isolation of suppressors of dksA rumA::CAT lethality.
Conducted extensive research in bacterial molecular biology regarding a key regulatory molecule ppGpp which is produced under starvation condition
Performed β- galactosidase assay on various mutants of E.coli
Generated clone to detect the suppressors of mutants RELEVANT EXPERIENCE
Quality Control Analyst
Intas Pharmaceuticals Pvt. Ltd, Ahemdabad, India July 2017- May 2018
Analyzed the quality of new drugs developed by performing various tests like pH check, osmolality test, SDS PAGE and western blotting
Acquired management skills by handling with the sale and purchase of chemicals and reagents for the industry ADDITIONAL EXPERIENCE
Graduate Teaching Assistant,
University of Massachusetts Lowell- Lowell, MA August 2019- Present
Teaching Assistant for Biol.4290/5290- Recombinant Protein Production Techniques under Dr Hwai-Chen Guo
Conducting lab for undergraduate and graduate students teaching transformation, Ni-NTA affinity chromatography, cell culture, FPLC and SDS-PAGE
Prepare assignments and exams for this course
Learned time management and leadership skills by teaching students