Dr. M.K.Kalaivani

Research Executive

CisGEN Biotech Discoveries Pvt. Ltd adcit4@r.postjobfree.com

Bioincubator, IITM madras research Park, +919********* Taramani, Chennai

Personal Summary

Technically astute, dynamic and highly focused person with extensive exposure to modern scientific technologies and broad set of scientific analytic skills.

Consistently strive to create a motivational and engaging learning environment for students which help students to achieve their own passion. Education

Name and address of

Institution/College

University/

Board

Degree Specialization

Year of

Passing

Faculty of Biomedical

Sciences technology and

Research

Sri Ramachandra

Institute of Higher

Education and

Research

Ph.D.

Medicinal plant

and Nephrology

2019

School of Biotechnology

and Genetic Engineering

Bharathiyar University

M.Sc.

Industrial

Biotechnology

2010

Prince Shri venkateshwara

Arts and Science College

Madras University

B.Sc.

Biotechnology 2008

Ph.D Thesis title: “Ameliorative effect of Boerhavia diffusa against Calcineurin Inhibitors induced Nephrotoxicity"

Research Experience

December 2018 to till now working as Research Executive in CisGEN Biotech Discoveries Pvt Ltd, Chennai, India.

Adaptation of virus in insect and canine cell lines

Expression, isolation and purification of recombinant protein from Baculovirus infected in SF-9 insect line.

Antigenic preparation from the isolated viral protein and Immunizing the chicken

Isolation and purification of IgY from immunized chicken

Isolation, identification and molecular characterization of probiotic organisms and large scale production for commercial purpose.

From June 2010 to November 2018 worked as Doctoral Research Scholar in the Department of Biomedical Sciences at Sri Ramachandra Institute of Higher Education and Research, Chennai, India.

Cell culture Experience: Expertise in the field of cell culture and development of primary cell culture system from human and rabbit. Maintenance of various mammalian cell lines including MDCK, U373MG, 3T3-L1, LLCPK1, MCF-7, A459, MDA-MB-231, HT-29. Molecular Biology: Isolation of DNA, RNA and proteins from various tissues; PCR and western blotting.

Flow cytometry: Analysis and interpretation of flow cytometry for cell cycle analysis, studying the different levels of apoptosis, ROS measurement and detection of mitochondrial membrane potential.

Biochemistry:Estimation of various antioxidant enzymes (Conventional method) such as total protein, SOD, Catalase, Glutathione reduced (GSH), Glutathione peroxidase (GPx). Phytochemistry: Isolation of phyto compounds from plant extracts using column chromatography. Thin layer chromatography.

SOP procedure preparation for the cell culture laboratory, Molecular biology and Microbiology labs.

Teaching experiences

Experience in teaching field for the following subjects medical physiology, endocrinology, microbiology, tissue engineering and biochemistry.

Experience in research assistance for various projects including summer research fellowship, value added courses and UG/PG final year projects.

Experience in NEET coaching for past three years.

Mentored Undergraduate and Post graduate students in Department of Biomedical Sciences for the following projects

Anticancer activity of Pedalium murex against lung cancer cell line A549 (UG Summer research fellowship)

Anticaner activity of Croton bonplandianum against lung cancer cell line A549 (M.Sc., Project Dissertation)

Alpha amylase activity of ethanolic extract of Croton bonplandianum (UG Summer research fellowship).

Anti-hyperglycemic and antioxidant potential of Croton bonplandianus. Bail fractions(UG Summer research fellowship).

Nephroprotective activity of ferulic acid against cyclosporine induced nephrotoxicity in MDCK cell lines (PG Internship).

Computer Skills

MsOffice suite, Graph pad prism, Mendeley, Chemsketch, Endnote. Seminars and Publications

PAPER PUBLICATION

1. Sumathy.Arockiasamy, Vinu.Ramachandran, Kalaivani.M.Kuppuswamy. Antiproliferative activity of methanolic fruit extract of Pedalium murex Linn against Lung Cancer Cell Line A549. Journal of Pharmacy Research 2012, 5(5), 2861-2866. 2. Kalaivani.M.Kuppuswamy,Bhavana jonnalagadda and Sumathy Arockiasamy. GC-MS analysis of chloroform extract of croton bonplandianum. Int J Pharm Bio Sci 2013 Oct;

(4): (P) 613-617.

3. G.Keerthana, M.K. Kalaivani, A.Sumathy. In-Vitro alpha amylase inhibitory and anti- oxidant activities of ethanolic leaf extract of Croton bonplandianum. Asian J Pharm Clin Res, Vol 6, Suppl 4, 2013, 32-36 (IF-0.17) .

4. M.K.Kalaivani, P.Soundararajan, Hannah. R. Vasanthi, A. Sumathy. In-Vitro nephroprotective role of ethanolic root extract of B.diffusa against cisplatin-induced nephrotoxicity. International journal of phytomedicine 7 (2015), 388-395. 5. Bhavana J, Kalaivani M.K andSumathy A. Cytotoxic and pro-apoptotic activities of leaf extract of Croton bonplandianusBail. Against lung cancer cell line A549. Indian Journal of Experimental Biology. Vol. 54, June 2016, pp. 379-385 (IF-0.934). 6. Uma Dharshini Karuppiah Vijayamuthuramalingam, Rajeswari Rajaram, Kalaivani Madhavaram Kuppusamy, Bhavana Jonnalagadda, Sumathy Arokiasamy. Anti- hyperglycemic and antioxidant potential of Croton bonplandianus. Bail fractions in correlation with polyphenol content. Iranian journal of basic medical sciences. Vol.20

(12), 2017, 1390 (IF-1.854).

7. M. K. Kalaivani, Sumathy Arockiasamy, Cordelia John, Hannah Rachel Vasanthi1, P. Soundararajan. Therapeutic Potential of Boerhavia diffusa L. against Cyclosporine A Induced Mitochondrial Dysfunction andApoptosis in Madin–Darby Canine Kidney Cells. Pharmacognosy magazine. Vol. 14 (55), 2018, 132-140 (IF-1.26). ORAL PRESENTATION

M.K.Kalaivani, Dr. J. Angayarkanni.“Production optimization and characterization of pterindeaminase from Micrococcus luteus” in the National level seminar “Biogalaxia 11” held on 14

th

October 2011 in Bharathiar University, Coimbatore.

M.K.Kalaivani, J. Bhavana, Dr. A. Sumathy. “Anticancer activity of Croton bonplandianum against lung cancer cell line A549” in the national seminar on

“Phytochemical as Therapeutics” held on 24

th

and 25

th

February 2012 organized by the

Department of Medical Biochemistry, University of Madras.

M.K.Kalaivani, Dr. A. Sumathy. “Attenuation of intrinsic apoptotic pathway in tacrolimus induced nephrotoxicity by Boerhaaviadiffusain MDCK cell line” in the national seminar BRCP-2017 held at Meenakshi College for Women in 30 th

& 31

st

January, 2017.

M.K.Kalaivani, Dr. A. Sumathy. “ Therapeutic effect of B.diffusa against Calcineurin inhibitors induced nephrotoxicity” in the 160th year post-centenary Diamond Jubilee celebrations on The international conference on Nutraceuticals- Concepts & Applications in Diseases on 16th February 2018 held at Department of Biochemistry, Madras University. (Best Paper Presentation Award).

POSTER PRESENTATION

M.K.Kalaivani, Dr. A. Sumathy, Dr. Hannah R Vasanthi, Dr. P. Soundararajan. “In-vitro antioxidant and nephroprective role of Boerhaaviadiffusa against cisplatin induced nephrotoxicity” in the Third International Seminar on Sustainable Utilization of Tropical Plant Biomass Through Biotechnology (SUPBT 2012), organized by K.V.M. college of Engineering & Information Technology.

M.K.Kalaivani, Dr. A. Sumathy, Dr. Hannah R Vasanthi, Dr. P. Soundararajan.

“Protective Effect of Boerhaaviadiffusaethanolic Root Extract Against Cisplatin&Tacrolimus Induced Nephrotoxicity” in the 9 th

National Research Scholars

Meet- 2013 held at Advanced Centre for Treatment, Research and Education in Cancer

(ACTREC).

M.K.Kalaivani, Dr. A. Sumathy, Dr. Hannah R Vasanthi, Dr. P. Soundararajan.

“Nephroprotective role of Boerhaaviadiffusaroot against FK506 induced Nephrotoxicity” in the 34

th

annual conference of IABMS and National symposium on “Role of Natural Resource in Health care”, held at Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani campus(Best poster presentation award).

M.K.Kalaivani, Dr. A. Sumathy, Dr. Hannah R Vasanthi, Dr. P. Soundararajan.

“Protective effect of Boerhaaviadiffusa against Cyclosporine induced nephrotoxicity” in the 4th Indian Academy of Biomedical Sciences Conference-2015 (IABS-2015)held at CSIR-IICT auditorium,Tarnaka, Hyderabad.

WORKSHOP ATTENDED

Participated in ‘Advanced GCP workshop’ conducted by SRU-Pfizer organized by Sri Ramachandra University from November 26

th

& 27

th

, 2011.

Participated in National level workshop on ‘Techniques in Bioactive Metabolites from Marine and other Bioresources’ organized by AMET university from December 12 th

–

16

th

, 2011.

Participated in National level workshop on ‘Quality sustenance in Research Publications’ organized by IQAC, Sri Ramachandra University on June 4 th

&5

th

, 2012.

Participated in ‘14

th

Indo-US flow cytometry workshop’ conducted by Sri Ramachandra University from October 28

th

to 30

th

, 2013.

References:

1) Dr. A. Sumathy,

Professor

Department of Biomedical Sciences

Sri Ramachandra Institute of Higher Education and Research Porur, Chennai 600 116.

Ph. No. 944-***-****

E.mail Id: adcit4@r.postjobfree.com

2) Dr. P. Soundararajan

Nephorologist

Sooriya Hospital

Vadapalani.

Ph. No. 984*******

E.mail id: adcit4@r.postjobfree.com

3) Dr. A. Hannah Rachel Vasanthi,

Professor& Head

Department of Biotechnology,

Pondicherry Central University.

Puducherry. 605014.

Mobile-944-***-****.

Email Id: adcit4@r.postjobfree.com

Personal details

Husband’s Name : Selvaraj Siva kumar

Date of Birth : 03-12-1986

Nationality Indian

Address No.10, Durairaj street, Gandhi Nagar, Nagelkeni, Chennai 44. I hereby declare that the above-mentioned details are true to the best of my knowledge.

(M.K.Kalaivani)

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