Mohammad Javed Equbal, PhD

* **** *******: March 5, 2020

Curriculum Vitae

Mohammad Javed Equbal

Email: adchd4@r.postjobfree.com

Mobile: +91-991.046.7840

Skype: javed.iitd

Nationality: Indian

Address: 161A, Moh. Mandai

P.O. Sasaram

State- Bihar (India).

PIN 821115

WORK EXPERIENCE

Assistant Professor, Tenure-Track (June 2016 — August 2018) Texas A&M University—Commerce, TX, United States

Biomedical Institute for Regenerative Research (BIRR) Major duties: Conducting research on stem cells and cardiac regeneration, Teaching, Department administration, Graduate students supervision, Laboratory compliance, Writing and submission of research grant proposals to major funding agencies, etc. Supervisor: Prof. Larry F. Lemanski (adchd4@r.postjobfree.com) Emolument: $80,000 p.a. plus Health and Retirement Benefits Postdoctoral Research Associate (August 2014 — May 2016) Indian Institute of Technology Delhi, India

Kusuma School of Biological Science (KSBS)

Major duties: Conducting research on chaperone assisted protein folding, Mentoring graduate students, Writing manuscripts, etc.

Supervisor: Prof. Tapan K. Chaudhuri (adchd4@r.postjobfree.com) Emolument: Rs.36,000 p.m. plus HRA

Postdoctoral Research Associate (August 2013 — May 2014) Indian Institute of Technology Delhi, India

Department of Biochemical Engineering and Biotechnology (DBEB) Major duties: Conducting research on developing novel strategies for overcoming protein aggregation, Mentoring graduate students, etc.

Supervisor: Prof. Prashant Mishra (adchd4@r.postjobfree.com) Emolument: Rs.22,000 p.m. plus HRA

Graduate Teaching Assistant (PT) (January 2008 — December 2012) Indian Institute of Technology Delhi, India

Department of Biochemical Engineering and Biotechnology (DBEB) Major duties: Assisting assigned faculty member(s) for their courses through taking graduate level laboratory classes, any other duty assigned by the department or faculty. Supervisor: Prof. Gopal P. Agarwal (adchd4@r.postjobfree.com) Emolument: Rs.20,000 p.m. plus HRA

Visiting Research Fellow (July 2011 — October 2011) Ecole Polytechnique Fédérale de Lausanne (EPFL), Switzerland Major duties: Conducting research on understanding neuroprotective role of miR-21in Huntington’s Disease model.

Supervisor: Prof. Ruth Luthi-Carter (adchd4@r.postjobfree.com). Relocated to University of Leicester, UK. Emolument: CHF 1650 p.m.

Junior Research Fellow (January 2007 — December 2007) Indian Institute of Technology Delhi, India

Department of Biochemical Engineering and Biotechnology (DBEB) Major duties: Conducting research on development of novel protein expression systems. Supervisor: Prof. Gopal P. Agarwal (adchd4@r.postjobfree.com) Emolument: Rs.8,000 p.m. plus HRA

Mohammad Javed Equbal, PhD

2 Last updated: March 5, 2020

Junior Research Fellow (March 2006 — December 2006) CSIR- Central Drug Research Institute, Lucknow, India Major duties: Conducting research on validation and development of recently identified drug targets for Leishmaniasis.

Supervisor(s): Dr. Neeloo Singh and Dr. Neeraj Sinha Emolument: Rs.8,000 p.m. plus HRA

EDUCATION

Doctor of Philosophy (Ph.D.) (January 2007 — March 2014) Indian Institute of Technology Delhi, India

Specialization: Biochemical Engineering and Biotechnology Thesis: Development of Novel Expression Systems in Escherichia coli and Corynebacteria Thesis submitted: July 2013 Degree awarded: March 2014 Division: BY THESIS (Course work CGPA: 8.67/10.0)

Master of Technology (M.Tech.) (July 2003 — September 2005) Anna University, Chennai, India

Specialization: Biotechnology

Dissertation: Cloning and Expression of human tissue plasminogen activator in Escherichia coli Division: FIRST (CGPA: 7.3/10.0)

Master of Science (M.Sc.) (January 2000 — November 2002) Patna University, Chennai, India

Specialization: Bioscience/ (Cytogenetics: 1 year or 50% of the course) Division: FIRST (Percentage: 72.75%)

Bachelor of Science, Honours (B.Sc., Hons.) (July 1995 — July 1999) Veer Kunwar Singh University, Arrah, India

Subjects: Botany, Chemistry, Zoology

Division: FIRST (Percentage: 66.87%)

Intermediate of Science (I.Sc. or 12TH) (July 1993 — June 1995) Bihar Intermediate Education Council, Patna

Subjects: Physics, Chemistry, Mathematics, Biology, English Division: FIRST (Percentage: 63.22%)

FELLOWSHIPS AND AWARDS

2017 Faculty Research Enhancement Program (FREP) Grant by TAMU−Commerce, USA 2017 Rashtriya Gaurav Award by IIFS, India (For contribution in science) 2014—16 Research Associateship by Department of Science and Technology, India 2013—14 Research Associateship by Department of Biotechnology, India 2011 Indo-Swiss Joint Research Fellowship by EPFL, Switzerland 2008—12 Teaching Assistantship by Indian Institute of Technology Delhi, India 2005—07 Junior Research Fellowship by Department of Biotechnology, India 2003—05 CEEB JNU MTech Fellowship by Department of Biotechnology, India PROFESSIONAL MEMBERSHIP AND SERVICES

Member, Indian Immunology Society (IIS), India Life member Member, American Heart Association, United States 2017- Member, International Society for Stem Cell Research, United States 2016- Associate Editor, Biology and Medicine (Longdom Publishing) 2018- Associate Editor, Heart Science and Heart Surgery Journal (HSHSJ) 2018- Associate Editor, Journal of Medicine and Biological Studies (JMBS) 2018- Mohammad Javed Equbal, PhD

3 Last updated: March 5, 2020

Member, Editorial Board – AASCIT- Frontier of Biomedical Sciences 2019- Serving as Reviewer for, but not limited to, Archives of Microbiology, BMC Microbiology, Environmental Toxicology, Journal of Cellular Physiology, Journal of Cellular Biochemistry, Reviews of Cardiovascular Medicine, Frontiers in Oncology, etc. CONTRIBUTION TO SCIENCE

BIOENGINEERING. During my doctoral research, the main focus of doctoral research work was to develop novel protein expression systems and metabolic engineering tools for Escherichia coli and Corynebacteria. For the very first time I developed T7 promoter based expression system for Corynebacterium sp., a close relative Mycobacterium. The arabinose inducible vector systems for industrially important Corynebacterium spp. were developed for metabolic engineering purposes. I also developed auto-induced T7 system for E. coli for cost-effective production of recombinant proteins.

a. Equbal MJ, Srivastava P, Agarwal GP, Deb JK (2013) Novel expression system for Corynebacterium acetoacidophilum and Escherichia coli based on the T7 RNA polymerase- dependent promoter. Appl Microbiol Biotechnol 97(17):7755-7766. (doi: 10.1007/s00253-013-4900-3) b. Equbal MJ, Agarwal GP, Deb JK (2019, tentative) A tightly regulated expression system in an industrially important Corynebacterium sp. (To be communicated) c. Equbal MJ (2013) Development of Novel Expression Systems in Escherichia coli and Corynebacteria. I.I.T. Delhi PhD Thesis Archive, India. TH 602.3 EQU-D. PROTEIN SCIENCE. During my postdoctoral tenure at IIT Delhi, I worked on chaperone assisted folding of multidomain aggregation prone proteins(s) both in vitro and in vivo conditions. I investigated in vitro and in vivo folding behaviour as well as mechanism of action of large, multi- domain, aggregation prone protein, maltodextrin glucosidase, MalZ, with GroEL and its apical domain (mini-chaperone) assistance. I characterized a small chaperone of Mycobacterium indicus pranii and its immunodominant properties. I also worked on refolding inclusion bodies of therapeutically important human interferon gamma using paramagnetic Fe3O4 nanoparticles. a. Sharma A, Equbal MJ, Pandey S, Sheikh JA, Ehtesham NZ, Hasnain SE, Chaudhuri TK (2017) Immuno-dominant protein MIP_05962 from Mycobacterium indicus pranii displays chaperone activity. FEBS J 284(9):1338-1354. (doi: 10.1111/febs.14057) b. Pastor A, Singh AK, Shukla PK, Equbal MJ, Malik ST, Singh TP, Chaudhuri TK (2016) Role of N-terminal region of Escherichia coli Maltodextrin Glucosidase in folding and function of the protein. BBA - Proteins and Proteomics 1864(9):1138-1151. (doi: 10.1016/j.bbapap.2016.06.008) c. Ahmad A, Equbal MJ, Khan JM (2015) Nanotechnology as next generation therapeutics: Hope for cancer treatment. Book Chapter in Modern Technology: Present and Future of Cancer. page 36-65, OMICS eBooks, ISBN: 978-1-63278-073-7. (doi: 10.4172/978-1-63278-073-7-074) NEUROBIOLOGY AND miRNA. Worked at Ecole Polytechnique Fédérale de Lausanne (EPFL), Switzerland on a research project entitled "Gene target prediction and validation for miR-22 mediated neuroprotection in rat striatal neuron culture". Using HTT-diseased model and 3-NP toxin model in cultured rat neurons, neuroprotective role of overexpressed miR-22 via general anti-apoptotic effects were elucidated out.

a. Equbal MJ, Luthi-Carter R (2011) A study on gene target prediction and validation for miR- 22 mediated neuroprotection in rat striatal neuron culture. EPFL Switzerland. DRUG TARGET DISCOVERY AND DEVELOPMENT. I worked on project entitled

"Identification and validation of new drug targets for Leishmaniasis" at Central Drug Research Institute, India for about a year. Working on this project I partially characterized of the ‘long chain fatty acyl CoA synthase’ of Leishmania donovani towards a potential drug target for Leishmaniasis.

Mohammad Javed Equbal, PhD

4 Last updated: March 5, 2020

REGENERATIVE MEDICINE & STEM CELLS. I was hired at Texas A&M University−Commerce as a tenure-track faculty. In Texas A&M, I along with my three students worked on different cellular and molecular biology related mechanistic aspects of direct cardiac reprogramming of human fibroblasts. I submitted few grant proposals to major funding agencies and was successful on one occasion. I guided thesis projects of my students in this area of research. a. Ravindran R, Kumar S, Rajkumar J, Roy S, Sathiya S, Babu CS, Equbal MJ* (2018) Inhibition of PKCβ Mediates Cardioprotective Activity of Ambrex against Isoproterenol-Induced Myocardial Necrosis: in vivo and in silico Studies. Biol Med 10(4):1-11. (doi: 10.4172/0974- 8369.1000442) [* Corresponding Author]

b. Zhao Z, Hong L, Rodriguez YL, Equbal MJ, Lemanski LF (2018) Repair of injured cardiac tissue using human-heart RNA in rodent myocardial infarction model. Annual Meeting of Biomedical Engineering Society 2018, Atlanta, United States. c. Ansari MA, Khan FB 2, Alam A, Tufail S, Khalique G, Equbal MJ, Alzohairy MA, Almatroudi A, Alomary MN, Pottoo FH (2020) Recent Nano-based Therapeutic Intervention of Bioactive Sesquiterpenes: Prospects in Cancer Therapeutics. Curr Pharm Des [Online ahead of print]. doi: 10.2174/1381612826666200116151522.

d. Lemanski LF, Kochegarov A, Neal M, Arms A, Rodriguez YL, Hong L, Equbal MJ, Biswas P, Biswas P, Gonzales M, Ross-Ferguson J, Zhao Z (2020, tentative), Differentiation of Mouse Embryonic Fibroblasts (MEFs) into Cardiomyocytes Using Human- Derived Cardiac Inducing RNA (CIR). (Communicated)

e. Gonzales M, Lemanski LF, Equbal MJ (2020, tentative) Current status of direct cardiac reprogramming: A review (Under preparation).

RESEARCH SUPPORT

Competitive Grant under Faculty Research Enhancement Program (FREP) Title: Generation of a human fibroblast cell line for screening of cardiomyocytes reprogramming factors/ co-factors.

Funding Agency: Texas A&M University—Commerce; Amount: $10,000 Period: 01/09/2017 - 31/08/2018 (Completed)

Role: Principal Investigator

STUDENT SUPERVISION

— Matthew Gonzalez (Graduate student: MS Thesis) July 2017 – Aug 2018 MS Thesis: Screening of Reprogramming Factors from Human Fetal Heart cDNA Library for Direct Cardiac Reprogramming.

— Fatima Sabti (Undergraduate student: BS Thesis) Sep 2017 – Aug 2018

— Sarah Sabti (Undergraduate student: BS Thesis) Sep 2017 – Aug 2018 ADDITIONAL INFORMATION (Conferences/Workshops/Trainings, etc.)

— Served as a Invited Judge for undergraduate and graduate student research competitions for Texas A&M University System Research Symposia, 2017.

— Attended a workshop on Grant Proposal Writing sponsored by the Texas A&M University— Commerce, TX, USA. (One-hour meeting per week during Sep.-Dec. 2017)

— Participated in the pre-meeting workshop on Clinical Translations of International Society for Stem Cell Research (ISSCR 2016) on June 21, 2016 at San Francisco, CA, USA.

— Attended 14th annual meeting of International Society for Stem Cell Research (ISSCR 2016) from June 22-25, 2016 at San Francisco, CA, United States. Mohammad Javed Equbal, PhD

5 Last updated: March 5, 2020

— Attended 4th annual conference “Bioworld 2014: Protein Structure and Folding" from Dec. 12-14, 2014 organized by Kusuma School of Biological Sciences, Indian Institute of Technology Delhi, India.

— Participated in the Indo-UK workshop on Next Generation Sequencing and Data Analysis for Microbial Applications from March 29-30, 2012 jointly organized by the Newcastle University, UK and IIT Delhi, India.

— Attended 1st annual conference “Bioworld 2011: Nucleic Acids in Diseases and Disorders” from Dec. 07-09, 2011 organized by Kusuma School of Biological Sciences, Indian Institute of Technology Delhi, India.

— Participated in the Short-Term Course on Bioinformatics from March 12-13, 2009 organized by Indian Institute of Technology Delhi, India.

— Participated in the Indo-UK workshop on “A Molecular Biology Approach for Environmental Engineering” from Feb. 23-27, 2009 jointly organized by the Newcastle University, UK and Indian Institute of Technology Delhi, India.

— Attended 31st Annual Conference of Indian Immunology Society from Dec. 16-18, 2004 organized by Anna University, Chennai, India.

MAJOR LABORATORY SKILLS

— Mammalian cells culture including iPSCs. Reprogramming of Fibroblasts into iPSCs.

— Plasmid vectors engineering, genome editing.

— Retroviruses/lentiviruses preparation and maintenance, Transduction.

— In vitro transcription; RNA, DNA, and protein extraction from various types of cells and tissues.

— Molecular cloning, plasmid engineering, gene synthesis.

— Standard microbiology techniques, strain engineering.

— Standard molecular biology techniques, PAGE, WB, ELISA, etc.

— Confocal microscopy of cells and tissue sections including live cell imaging on Olympus CM. Trainings taken at TAMU-Commerce (By certification)

— Basic Aseptic surgery Training (Basic Course)

— Basic Biosafety Training (Stage 1)

— Biomedical Responsible Conduct of Research

— Introduction to Biosafety Training (Stage 1)

— Reducing Pain and Distress in Laboratory Mice and Rats (Basic Course)

— Working with Mice in Research Settings (Basic Course)

— Working with Rats in Research Settings (Basic Course)

— Working with the IACUC (Basic Course)

DECLARATION

I hereby declare that the information given is correct to the best of my knowledge and belief.

(Mohammad Javed Equbal)

CONTACT REFERENCE

SUPERVISORS

1) Prof. Larry F. Lemanski (Relationship: Supervisor in Texas A&M and Director of the Institute) Distinguished Research Professor, TAMU System Regents Professor, and Director of the BIRR Biomedical Institute for Regenerative Research (BIRR), Texas A&M University-Commerce, 2600 S. Neal St., Commerce 75428, TX, United States. Email: adchd4@r.postjobfree.com Tel. +1-903-***-**** 2) Prof. Gopal P. Agarwal (Relationship: Teacher & Doctoral Advisor) Emeritus Professor

Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India Email: adchd4@r.postjobfree.com Tel. +91-11-265*-**** 3) Prof. Tapan K. Chaudhuri (Relationship: Teacher & Postdoctoral Advisor) Professor (Full)

Kusuma School of Biological Sciences,

Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India. Email: adchd4@r.postjobfree.com Tel. +91-11-265*-**** COLLEAGUES/ COLLABORATORS

4) Dr. Shaikh Z. Ahammad

Associate Professor

Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India Emai: adchd4@r.postjobfree.com Tel. +91-11-265*-****

5) Dr. Puneet Juneja

Core Director - Biochemistry Connector

Robert P. Apkarian Integrated Electron Microscopy Core Emory University, Dowman Drive, Atlanta, Georgia 30322, United States Email: adchd4@r.postjobfree.com Tel. +1-865-***-**** 6) Dr. Intekhab Alam

Research Associate

Department of Physiology and Biophysics, School of Medicine, Case Western Reserve University, 2109 Adelbert Road, Cleveland, OH 44106, United States Email: adchd4@r.postjobfree.com Tel. +1-216-***-****

Contact this candidate