FELECIA E. MANN

***** ********* *******

Germantown, MD 20876

301-***-****

e-mail: adbk7y@r.postjobfree.com

PROFESSIONAL SUMMARY

A hardworking and dependable professional experienced in providing administrative, managerial and technical support in Molecular Biology, Biochemistry, Virology and Histology laboratory environments. Ability to supervise, train, and evaluate laboratory technicians.

Skills: Cell/tissue cultures, DNA sequencing, DNA/RNA extractions; proficient in ELISA, Microsoft Word, Excel, PowerPoint, Outlook; ABI SeqScape, ABI Collection Data, ABI SDS, Matrix Platemate and Affymetrix GeneChip Software Programs.

EDUCATION

B.S., Biology, Virginia State University, Petersburg VA

Medical Laboratory Specialist Certification, Academy of Health Science US Army, Fort Sam Houston, TX

PROFESSIONAL EXPERIENCE

Owner, Mann! Water Works! Germantown, MD Sept. 2005 – Present

Clients: The Wellness Network, SC; H2O Fitness & More, Rockville, MD

Title: Aquatic Instructor- First Aid/CPR certified

Areas of Instruction:

Deep Water Running – An exercise that strengthens the core, arm and legs simultaneously. With the use of balls, noodles and bouncy hand bells. I put the students thru rigorous exercises that would get all muscle groups to work together against the resistance of the water creating a high cardio workout.

Shallow Water Exercise – Challenges students in multi-levels of aerobics using the resistance of the water to strengthen muscles, increase range of motion, balance and improve cardiovascular fitness.

Aqua Arthritis – Modified aqua exercises designed to keep the students’ feet on the pools’ floor while using the resistance of the water to increase their range of motion in their hips, legs, arms, hands and back muscles that would improve their balance. The water also softens the impact on their joints during exercise.

Water Aerobics – Fast-paced aerobics with a HI LOW intensity similar to boot camp classes. The high intensity workout in water cushions the feet, back and knees thereby placing emphasis on cardiovascular conditioning.

Research Biologist (Contract, Part-time) March 2009 – May 2009

Laboratory of Receptor Biology and Gene Expression, NIH/National Cancer Institute (NCI), Bethesda, MD

Set up two cell culture systems for new lab- Human cancer cells and Drosophila culture cells. Maintained, passaged and inventoried cells at least 3 times a week, and created backup frozen stocks of all cell lines in liquid Nitrogen. Created inventory list of all cell lines in the lab. Performed rigorous quality control on cell lines.

Assisted PIs on experiments and in growing cells for experiments Taught 2 students how to passage and culture human cancer cells.

Ordered and organized new products, equipment and protocols for newly established lab at NCI. Organized the lab efficiently, in compliance with NIH procedures, and streamlined system for the PI.

Assessed when and if bacterial infections occurred in Drosophila cell lines, including testing several batches of cells and media; trouble shot issues for remediation.

Assisted lab biologist on all lab administrative tasks.

Contractor, Kelly Services) Sept. 2002 – Sept. 2008

1.Client: Laboratory of Human Carcinogenesis, National Cancer Institute/NIH, Bethesda, MD (11/07-9/08)

Isolated RNA and DNA from normal and tumor tissue samples for gene mapping to determine whether there was lost in heterozygosity between the pairs of tissues.

2.Client: Laboratory of Population Genetics, NCI/NIH, Bethesda, MD (9/02 – 3/07)

Sequenced and analyzed samples for BRCA1 & BRCA2 mutations.

Processed tumor and normal tissue samples for isolation of total RNA. Prepared target probes for GeneChip hybridization.

Performed analysis of expression assays using Affymetrix GeneChip System. Created microarray slides of lung cancer tissue and performed immunohistochemistry for assay optimization in order to identify and characterize antibody markers.

Transfection of cell lines for characterization of various different molecular markers.

Dissected mice for various basic science research projects.

Celera Genomics, Rockville, MD 3/2000 – 4/2002

1.Research Associate, Biologics (2/2001 – 4/2002)

Managed the construction and organization of the Biologics Department.

Prepared peptides and DNA for inoculations.

Complied with the Animal Welfare Act relating to our use of mice and rabbits. Tested serum of mice and rabbits using ELISA assays for antibody responses.

Produced monoclonal and polyclonal antibodies in mice and rabbits.

Analyzed and presented graphical presentations of data.

Ensured safe use and disposal of biohazard material. Maintained a safe working environment for all. Procured materials and supplies as needed.

2.Production Assistant II (Shift Supervisor), Production/Cloning (3/2000 – 2/2002)

Worked closely with Team Leader to meet short- and long-term goal requirements of picking 540 colony plates and plating 300 plates daily.

Managed technical direction for a team of five to eight lab technicians. Performed all duties of the cloning lab by adhering to standard operating procedures.

Carried out transformation of DNA into E. coli on agar plates.

Maintained Q-bots and other lab instruments to ensure their proper function according to standard operating procedure and manufacturer specifications.

Research Assistant III, HIV Research Program Aug.1991 – Jan. 2000

Henry Jackson Foundation /Walter Reed Army Institute of Research

Supervised eight technicians and summer students.

Trained visiting scientists in our GP160 clinical trial experiment techniques.

Researched the HIV virus, generated data from specimens in the HIV vaccine GP160 clinical trial using proteins and peptides with quality control validation.

Achieved certification on ROCHE Assay 1.0, 1.5 and UltraSensitive testing serum of HIV patients.

Synthesized peptides used in HIV vaccine trial.

Managed chemical waste collection for all foundation labs at the Gude Drive location according to regulations.

Embedded and cut histology case blocks, performed special staining of slides

Wrote new and revised protocols as procedures changed.

Medical Laboratory Specialist

Walter Reed Army Hospital, Washington, DC April 1990 - April 1998

Sergeant (E-5) U.S. Army Reserve, 2290th Army Medical Field Hospital.

Obtained data from various patient urine and blood samples curated in chemistry, microbiology and blood bank.

Tests included urinalysis, glucose, RPR’s, pregnancy, serology, blood bank (immunology and hematology), WBC and RBC counts, phlebotomist (children and adults) Lipids - HDL profiles and processing of specimens.

Technician, Roche Biomedical Laboratories, Richmond, VA December 1988 - May 1990

Routine laboratory testing and procedures in chemistry. Prepared specimen and paperwork prior to actual testing.

Evaluated patient results for proper verification and approval by technologist or supervisor.

Maintained necessary documentation and logs of workload volume, quality control data, and maintenance and troubleshooting of instruments.

PUBLICATIONS

Mural RJ et al. 2002. A comparison of whole-genome shotgun-derived mouse chromosome 16 and the human genome. Science 296:1661-1671.

Yang et al. 2005. Mutations in the tyrosine kinase domain of the epidermal growth factor receptor in non-small cell lung cancer. Clinical Cancer Research 11:2106-2110.

Fukuoka J et al. 2007. Desmoglein 3 as a prognostic factor in lung cancer. Human Pathology 38(2): 276-283

Landi MT et al. 2008. Gene expression signature of cigarette smoking and its role in lung adenocarcinoma risk and survival. PLoS ONE. 2008 Feb 20, 3(2):e1651.

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