Molecular Biologist

CURRICULUM VITAE

Name and Surname: NGOM NGOM Justice Tresor

Gender and Age: Male,28 years old P.O. Box: 946 Base Aérienne 201, Douala Tel: +237-********* / +237-********* E-mail:***********@*****.*** Skype: Tresorngom LinkedIn:linkedin.com/in/justice-tresor-ngom-ngom-139a7b87 Researchgate: https://www.researchgate.net/profile/Justice_Ngom EDUCATION AND TRAINING

2014-2016 Postgraduate degree in Molecular and Cell Biology (Master Research) Faculty of Sciences, University of Douala

2013-2014 Master of Sciences in Molecular and Cell Biology Faculty of Sciences, University of Douala

2009-2013 Bachelor of Sciences in Biochemistry

Faculty of Sciences, University of Douala.

PROFESSIONAL EXPERIENCE

March 2017-Sept 2018 Project USAID-Emerging Threats Program Predict -2 /Metabiota, Cameroon and Centre for Army Health Research CRESAR; Yaoundé, Cameroon Position held: Research Laboratory Scientist Junior Highlights of responsibilities: Molecular Surveillance of Viral Zoonosis: DNA/RNA extraction, molecular analysis (real time PCR, RT PCR, nested and semi nested PCR), cDNA synthesis, Electrophoresis, specimen processing for SANGER, Sequencing: gel clean and gel purification and cloning. Stocks and Wastes management Oct 2016-Dec 2016 International Reference Chantal Biya / Molecular Biology Laboratory, Yaoundé, Cameroon. Virology and molecular biology units

Position held: Research Laboratory Scientist Junior Highlights of responsibilities: Management of laboratory, DNA/RNA extraction, molecular analysis (RT PCR, nested and semi nested PCR), cDNA synthesis, Electrophoresis Molecular Genotyping and Drug resistance test, SANGER Sequencing reaction. Bioinformatics and Phylogenic analysis,

June 2015- Dec 2015 Centre Pasteur of Cameroon, Yaoundé Cameroon. Reference Mycobacteriological Laboratory Position held: Research Laboratory Scientist Junior, Highlights of responsibilities:Medical analysis, Culture of mycobacteria and drug susceptibility test, Ziehl-Neelsen and Auramin staining, microscopy, DNA/RNA extraction, PCR, molecular hybridizationand other molecular analyses (GenXpert, Hain, Spoligotyping, MIRU, LSP). Bioinformatics and Phylogenic analyses,

Aug 2014- Nov 2014 Project USAID-Emerging Threats Program Predict -1 /; John Hopkins Cameroon, Global Viral Forecasting Initiative, Yaoundé Cameroun.

Position held: Research Laboratory Scientist Intern, Highlights of responsibilities: Molecular Surveillance of Viral Zoonosis: DNA/RNA extraction, molecular analysis (real time PCR, RT PCR, nested and semi nested PCR), cDNA synthesis, Electrophoresis, specimen processing for SANGER Sequencing: gel clean and gel purification. Sept 2013- Nov 2013 Military Health Regional Hospital 2 (RMH2), Douala, Cameroon. Position held: Laboratory Scientist Intern,

Highlights of responsibilities: Sample collection and Microbiological, haematological and virological analyses.

Aug 2013- Sept 2013 Institute for Agricultural Research for Development "(IRAD), Njombe, Cameroon. Position held:Laboratory Scientist Intern, Phytosanitary Service Highlights of responsibilities:Grafting, Phytosanitary processing ADMINISTRATIVE EXPERIENCE

Jan 2017- Nov 2017 University of Douala, Faculty of Sciences, Douala, Cameroon. Biochemistry Department Position held:Part time Lecturer in Bioinformatics and Molecular Biology, Highlights of responsibilities: Prepared and graded exams and quizzes Organized and ran laboratory practical sessions

RESEARCH EXPERIENCE

June 2015- June 2016 Centre Pasteur of Cameroon Yaoundé and University of Douala, Biochemistry Department Molecular epidemiology of extrapulmonary tuberculosis Position held: Co Investigator

Highlights of responsibilities Medical analysis, Culture of mycobacteria and drug susceptibility test, Ziehl-Neelsen and Auramin staining, microscopy, DNA/RNA extraction, PCR, Hybridization molecular and other molecular analysis (Genxpert, Hain, Spoligotyping, MIRU, LSP), Bioinformatics and Phylogenic analysis,

March 2017-Sept 2018 Project USAID-Emerging Threats Program Predict -2 /Metabiota, Cameroon and Centre for Army Health Research CRESAR; Yaoundé, Cameroon Position held:Laboratory Scientist Junior

Highlights of responsibilities: DNA/RNA extraction, molecular analysis (real time PCR, RT PCR, nested and semi nested PCR), cDNA synthesis, Electrophoresis, specimen processing for SANGER, Sequencing: gel clean and gel purification and cloning. Stocks and Wastes management

LABORATORY SKILLS AND TECHNIQUES

Collecting samples

Serology, Spectrophotometry(Biochemistry)

Bacteria/fungi culture and antibiogram

Enzyme Linked Immunosorbent Assay Media Preparation,Virus culture; cell culture Light microscopy, Hemoglobin/Agarose gel

electrophoresis, Immunochromatography

technics.

Nucleic acid extraction Polymerase Chain Reaction (PCR) RT-PCR Real time-PCR, CDNA synthesis

AFB, Molecular Hybridization, Genxpert, Hain,

Spoligotyping, MIRU, LSP,

Nucleic acid gel clean and purification, DNA Sequencing; sequences analysis,(Bio edit, MEGA 5, Geneious), Cloning, Primers designing Molecular Genotyping and Drug resistance

test

Phylogenetic analysis (MEGA, Bioedit)

Decontamination Metohds,

OTHER SKILLS

Handling samples Authorization in the laboratory security level type 2 to 3 (P2 and P3). Project and Event Management, Time and task management, Team building, Problem solving, SOPs Writing and application

(Standard Operating Procedures), Good Clinical Practice/Good Laboratory Practice Training (GCP/GLP), Statistical analyses with SPSS First aid technics and fire safety (Red cross, University of Douala), Sports and medical aid technics, Peer Educator, Saponification technics, Draft flyer and post. TRAINING AND DISSEMINATION OF RESEARCH RESULTS

Trainings and Courses

2019 - ICH Good Clinical and Laboratory Practice E6 (R2), Introduction to Reviewing Genomic Research and Ethics and Best Practices in Sharing Individual-Level Research Data, the Global Health Network 2019- Yaoundé Advanced Course in Immunology (YACI), University of Yaoundé, University of UTAH et CPC 2019- Advance Course on Viral Hemorrhagic fevers, Cameroonian Society for Microbiology et FMSB Yaoundé 2019- Advance Training on Molecular Diagnosis Of Viral Hemorrhagic Fevers as Trainer. USAID/PREDICT, Metabiota and CRESAR

Publications

1) Justice Trésor Ngom, Francioli Koro, Celestin Godwe, Clemence Adande, Romaric Ateugieu, Tatiana Onana, Annie Ngono, Yannick Kamdem, Sara Ngo-Niobe, François-Xavier Etoa. Genotypic diversity and drug susceptibility patterns among M. Tuberculosis complex isolates responsible of extrapulmonary tuberculosis in cameroon from 2006–2015.BMJ Global Health Feb 2017, 2 (Suppl 2) A44; DOI: 10.1136/bmjgh-2016-000260.116 2) Etoundi Omgba Cunégonde Blanche, Mbock Émilie Danielle, Djopnang Djimbie Justin, Moussambe Abanga Agathe and Ngom Ngom Trésor.Glycaemic Index and Macronutrients Content of Three Traditional Cameroonian Meals.Journal of Food, Nutrition and Population Health. 2017. Vol.1 No.2:20http://www.imedpub.com/food-nutrition-and- population- health/.

3) Charles Fokunang Ntungwen Marcellin Ndoe Guiaro, Jean-Patrick Molu, Djoulde Ibrahima, Mansour Mohamadou, Michele Corneille Matchim Kamdem, Cyrille Levis Kountchou, Ibrahim Mbarawa Kofia, Justice Tresor Ngom Ngom, Joel Brice Ngoupa, Julius Mbekem Nwobegahay.Beneficial effect of HIV-2 on the progression of HIV- 1-associated nephropathy among patients under HAART. J Anal Pharm Res.Aug 2018,;7(4):437 444. DOI: 10.15406/japlr.2018.07.00263. Présentations Poster

1) Justice Trésor Ngom, Francioli Koro, Celestin Godwe, Clemence Adande, Romaric Ateugieu, Tatiana Onana, Annie Ngono, Yannick Kamdem, Sara Ngo-Niobe, François-Xavier Etoa. Genotypic diversity and drug susceptibility patterns among M. Tuberculosis complex isolates responsible of extrapulmonary tuberculosis in cameroon from 2006–2015. 8thEDCTP International Forum: Defeating poverty-related and neglected diseases in Africa: harnessing research for evidence-informed policies at LUSAKA, ZAMBIA 6-9 November 2016 PERSONAL SKILLS AND COMPETENCES

Driving licence categoriys “B”

Language(s) French and English

Social skills and experiencesTeam spirit and collaboration, Good leadership skills, Good ability to adapt to multicultural environment gained through: Funder of the club of biotechnology at University Douala, treasury of educational pars health club, Red cross club Technical Advisor, member of the Olympic club, University of Douala, Volunteer atAIESSEC Cameroon since November 2015, health campaign in West Cameroon region (Dschang 2016, Bafang 2017 Mbouda 2018): Technical Advisor and Chair, volunteer in a rural community OWP (one world project) in the Littoral and Center

(2016 and 2018) country based on education, health, environment and enjoy participation.Volunteer atHealth and Development in Action (HEADA),

Organisational skills: Shows great leadership ability, have good organizational experience. Objective and focused. Sticks to time and always striving the target. I have been a regular organizer of several Seminars, training and workshop. Mastering in report writing

Computer skills and HobbiesVery good in computing like Microsoft options such as Microsoft windows, Microsoft office, word, Access, PowerPoint, publisher, XLSTAT. Reading; Travel; Sport; Music and Movies. AWARDS, GRANTS AND HONORS

The 2017, 2014, 2013, 2012 President of the Republic of Cameroon Prize for the Best Postgraduate, Master student, Bachelor and Undergraduate student.

PROFESSIONAL AFFILIATIONS

2016-2018 European Society of Clinical Microbiology and Infectious Diseases (ESCMID). 2016-present Member of the International Society of Infectious Diseases (ISID) 2018-present Member of Health and Development in Action (HEADA) 2019 Initiative to Strengthen Health Research Capacity in Africa (ISHReCA) REFEREES

Pr NGONO NGANE Anne Rosalie Head of Biochemistry Laboratory and Department (University of Douala) Faculty of Sciences P.O. Box: 24157 Douala Cameroun Email: ******@*****.*** Tel: +273-********* Pr Judith TORIMIRO Head of Molecular Biology Laboratory of International reference Centre Chantal BIYA P.O. Box: 3077 Yaoundé Cameroon Email: *********@*****.**.** Tel: +237-********* Dr Sara EYANGO,PhD HDR Scientific Director of Centre Pasteur du Cameroun Po box 1274 Yaoundé Email: ***********@*****.** Tel: +237-*********

Dr Francioli KORO KORO,PhD Senior Lecturer (University of Douala) Faculty of Sciences Po. Box: 24157 Douala Cameroun Email: *************@*****.** Tel: +237-********* / +237-********* Dr MOUICHE Moctar DMV PhD Country Director Project USAID-Emerging Threats Program Predict Email: ********@******.*****: +237-*********

Dr NGUM NDZE Valentine, PhD Laboratory Coordinator at Metabiota Cameroon Po. Box 7039 Yaoundé Cameroon Email: *************@*****.*** Tel: +237-********* Colonel Dr. Julius MBEKEM NWOBEGAHAY PhD Head of Centre for Army Health Research Po. Box 7039 Yaoundé Email: **********@*****.*** Tel: +237*********

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