Research Scientist Ph D

Philip H. Jones, PhD *

PHILIP H. JONES, PhD

*** **** *** ******, ***. A, Pittsburgh, PA 15221

TELEPHONE: 605-***-**** EMAIL: ***********@*****.*** PROFESSIONAL SUMMARY

My education and experience for the past 10 years involved developing murine models to study early-stages of viral and bacterial infections. I also possess strong verbal as well as written skills proven by several high impact publications as a first author. Also, I am an excellent research collaborator demonstrated by several co-authorships. AREAS OF EXPERTISE

Drug-resistant cancer cell line development

Multi-color flow cytometry and Western Blots

RNAi studies using siRNA, shRNA, and lentiviruses

Molecular cloning and cell line development

DNA sequencing and long-read analyses

Assay development for viral and bacterial pathogens

End point (TCID50) and drug synergy assays

Use of analytical and genomic software

EDUCATION

Doctor of Philosophy, Biological Sciences, University of South Dakota, Vermillion, SD Master of Science, Biological Sciences, Auburn University, Auburn, AL Bachelor of Science, Biology, Temple University, Philadelphia, PA Associate of Science, Laboratory Technology, Community College of Allegheny County, Pittsburgh, PA PROFESSIONAL EXPERIENCE

Research Associate 2021-Present

University of Pittsburgh Medical Center (UPMC), Magee Womens Research Institute, Pittsburgh, PA

• Identified drugs preventing DNA damage in ovarian and endometrical cancer cell lines

• Validated cancer treatments from survivalship curves and tumor volumes in immunosuppressed mice and cell lines

• Determined IC50 values and conducted synergy assays using chemotherapeutic and suppressor drugs on cell lines

• Created drug-resistance cell lines for drug efficacy assays

• Performed RNAi studies testing chemotherapeutic drug effects on targeted genes and their signaling pathways

• Provided technical expertise on troubleshooting experiments and/or data analyses

• Responsible for lab safety, training, budgeting, and purchasing using federal funds

• Supervised and trained post-doctorates, visiting faculty scholars, undergraduates, and high school students (14 total) Research Scientist 2018-2021

University of Pittsburgh, Department of Pediatrics, Neonatal Medicine, Pittsburgh, PA

Identified and isolated clinical mutations of targeted genes involved in regulating cell growth and differentiation

Sequenced primary cancer cells to analyze possible point mutations of targeted genes

Established knockout cell lines using shRNA technology

Isolated immune cells from Peyer’s Patches of wild-type and mutant mouse strains using multi-color flow cytometry Research Scientist 2017-2018

University of Pittsburgh, Department of Medicine/Pulmonary, Pittsburgh, PA

Created Pseudomonas aeruginosa infected mouse models to study impacts on lung tissue and bronchoalveolar lavage during acute lung injury

Harvested tissues, blood and bronchoalveolar lavage to quantify P. aeruginosa using agar-based cultures

Isolated RNA from tissue and blood to analyze cytokine and chemokine expression

Examined immune cell populations present in injured lungs by performing 7-color cell surface and intercellular staining Research Scientist 2015-2017

Advanced Genomic Technology/University of Louisville, Louisville, KY

Conducted laboratory experiments examining microRNA effects on obesity using transgenic mouse models

Developed a protocol for inducing mature adipocytes from mice stem cells

Performed RT-qPCR analyses to identify obesity-associated genes that leads to fat accumulation in mice Philip H. Jones, PhD 2

Successfully cloned microRNAs sequences into vectors for in vitro transfection experiments on primary cell lines

Validate cloned microRNAs by way of DNA sequencing and fluorescent microscopy Postdoctoral Research Scholar 2010-2015

University of Iowa, Department of Microbiology, Iowa City, IA

Conducted in vitro and in vivo research on the following viruses, HIV, Mouse Mammary Tumor Virus, and Chikungunya Virus, to examine efficacy against viral propagation

Established how the antiviral protein, BST-2 (Tetherin), regulates breast cancer development

Transfected VLPs (virus-like particles) into cells to determine which components regulate acute or chronic infections

Used RNAi (siRNA and shRNA) against BST-2 to understand the impact of antiviral genes on viral infections

Isolated and propagated viruses from cell cultures and oncolytic-induced breast tumors Graduate Research Assistant 2005-2010

University of South Dakota, Department of Biology, Vermillion, SD

Performed DNA sequencing to design DNA primers from unclassified ectoparasites (flea DNA) involved in the spread of bubonic plague (Yersinia pestis)

Acquired GenBank accession numbers and published all primers on the NCBI PubMed central database

Isolated flea DNA to test for the presence of Y. pestis using a PCR-based assay

Generated and analyzed multiple, large-scaled genetic databases

Characterized different strains of Y. pestis by way of DNA sequencing FELLOWSHIPS, GRANTS, AND AWARDS

NIH T32 Post-Doctoral Training grant in Parasitology, University of Iowa: 2013-14

NIH T32 Post-Doctoral Training grant in Immunology, University of Iowa: 2011-13

Graduate Research Grant, University of South Dakota: 2006 SELECTED PEER-REVIEWED PUBLICATIONS (9 of 16)

Al-Sudani H, Ni Y, Jones PH, et al. 2023. Targeting CD47-SIRPa axis shows potent preclinical anti-tumor activity as monotherapy and synergizes with PARP inhibition. NPJ Precision Oncology 69: 1-10.

Jones PH, Deng B, Winkler J, Zirnheld AL, Ehringer S, Shetty V, Cox M, Nguyen H, Shen W-J, Huang T-T, Wnag E. 2019. Over-expression of miR-34c leads to early-life visceral fat accumulation and insulin resistance. Scientific Reports 9: 13844.

Jones PH, Okeoma CM. 2015. Detection of Chikungunya virus (CHIKV) in urine of infected mice: A Potential Non- invasive Diagnostic Tool for CHIKV. Journal of Infectious Diseases & Therapy 3: 4.

Madison MN, Jones PH, Okeoma CM. 2015. Exosomes in human semen restrict HIV-1 transmission by primary vaginal cells and block intravaginal replication on LP-BM5 murine AIDS virus complex. Virology 482: 189-201.

Jones PH, Mahauad-Fernandez WD, Madison NM, Okeoma CM. 2013. BST-2/tetherin is overexpressed in mammary gland and tumor tissues in MMTV-induced mammary cancer. Virology 444: 124-39.

Jones PH, Maric M, Madison MN, Maury W, Roller RJ, Okeoma CM. 2013. BST-2/Tetherin-mediated restriction of Chikungunya (CHIKV) VLP budding is counteracted by CHIKV non-structural protein 1 (nsP1). Virology 438: 37-49.

Jones PH, Okeoma, CM. 2013. Phosphatidylinositol 3-kinase is involved in Toll-like receptor 4-mediated BST- 2/tetherin regulation. Cellular Signaling 25: 2752-61.

Jones PH, Mehta HV, Maric M, Roller RJ, Okeoma CM. 2012. Bone marrow stromal cell antigen 2 (BST-2) restricts mouse mammary tumor virus (MMTV) replication in vivo. Retrovirology 9: 10.

Jones, PH, Mehta HV, Okeoma CM. 2012. A novel role for APOBEC: susceptibility to sexual transmission of murine acquired immunodeficiency virus (mAIDS) is aggravated in APOBEC deficient mice. Retrovirology 9: 50. Full CV and Publications listing available upon request.

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