Quality Control Research Associate
Resume:
SANTHOSHI MOTHKURI
*****, ******** *** *****: *********.********@*****.*** Farmington Hills, MI - 48335 Mobile: +1-248-***-****
Summary
Hands on experience in DNA extraction, Amplification by PCR, Primer/Probe design.
Hands on experience in Genomic DNA Isolation and use of restriction enzymes.
Hands on experience in calibrate and perform routine maintenance on specialized equipment.
Hands on experience in Agarose gel electrophoresis, SDS-PAGE.
Analyse, record, and communicate test results in a timely manner.
Review test results for accuracy and maintained accurate quality control record.
Got the Merit certification in participating micro cosmos seminar.
Organized several LIFE SCIENCES meets.
Professional Experience
4+ years of experience in molecular technology related projects.
Worked as research associate in National Research Centre on Meat since 2013 to 2016.
Worked as a Quality Control Analyst in PARLE FOOD PRODUCTS PVT.LTD since 2012 to 2013.
Projects
Detection and Adulteration of animal derived materials in commercial Ghee samples
Equipment : Real-time PCR thermal cycler, Centrifuge, Agarose electrophoresis, Polyacrylamide
Electrophoresis.
Duration : June 2013 – Feb 2016
Location : National Research Center on Meat, Hyderabad, India
Description:
For the safety and quality of food products, ICAR (Indian Council of Agriculture Research) in collaboration with NRCM verifies the adulteration on different food products. In this project, the aim is to detect the adulteration in commercial ghee samples through molecular techniques. Based on the results from NRCM, ICAR will issue safety and quality ratings.
Responsibilities:
1.Collection of the samples time to time and store them under necessary temperature conditions.
2.Preparation of all necessary reagents for the next level procedure.
3.Isolation of DNA from the Ghee sample.
4.Amplification of extracted DNA through PCR.
5.Performance of Load and Run an Agarose Gel Electrophoresis.
6.Analysis of the results and reporting.
Species Identification from Forensic samples
Equipment : Real-time PCR thermal cycler, Centrifuge, Agarose electrophoresis, Polyacrylamide
Electrophoresis.
Duration : June 2013 – Feb 2016
Location : National Research Center on Meat, Hyderabad, India
Description:
The aim of this project is to identify the animal species and its sex from different input samples like it’s meat, hair or nails. This information is mainly needed by state police departments for their investigation on knowing if there is any protected animal was killed.
Responsibilities:
1.Preparing the storage conditions depending on the received animal sample type.
2.Preparation of all necessary reagents for the next level procedure based on the sample type.
3.Isolation of DNA using different DNA extraction techniques.
4.Amplification of extracted DNA through PCR.
5.Performance of Load and Run an Agarose Gel Electrophoresis.
6.Analysis of the results and reporting.
Estimation of Oxidation in Emu fat oil
Equipment : Separatory Funnel, Burettes, Pipettes, Microwave.
Duration : June 2013 – Feb 2014
Location : National Research Center on Meat, Hyderabad, India
Description:
Emu oil is high in omega-3, omega 6, and omega-9 fatty acids. These helps reduce inflammation, ease muscular pain, and arthritic joint pain. They may also help relieve signs of wrinkles, scars, and blemishes by nourishing the skin cells. The aim of this project is to estimate the Oxidation levels in Emu fat oil, which are used by the Pharmaceutical Industry.
Responsibilities:
1.Extraction of oil from Emu fat using the methods like liquid-liquid, micro-wave, heating.
2.Separation of impurities using solvent extraction.
3.Calculation of oxidation levels using different methods.
Verification of raw materials quality and manufacturing process for PARLE products
Equipment : Hot air Oven, Separatory Funnel, Burettes, Pipettes.
Duration : Nov 2012 – June 2013
Location : PARLE LIMITED&CO, Hyderabad, India
Description:
PARLE is a popular food products company based in INDIA. It owns the famous cookies and biscuit products. The aim of this project is to check the quality of raw materials like all-purpose flour, Sugar, Vanaspati and Milk powder. It also verifies the guidelines for manufacturing process.
Responsibilities:
1.Physical examination of raw materials for their texture, taste, smell, and colour.
2.Sedimentation of raw materials.
3.Calculation of quality parameters like gluten percentage, moisturizer percentage, PV, FFA and unsaturated fatty acids.
4.Supervising the manufacturing process.
5.Examining the final product and supervising the packaging.
6.Providing all the documentation for periodic audits.
Education Profile
Completed Master of Science in Microbiology from Sreenidhi P.G. college, Osmania university, Telangana, INDIA in 2012.
Completed Bachelor of Science in Biochemistry, Microbiology, Chemistry from R.B.V.R.R. Women’s degree college, Osmania university, Telangana, INDIA in 2010.
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