Laboratory Technician Molecular Biology

MUHAMMAD QARI ABSAR ALI

+1-437-***-**** **********@*****.*** www.linkedin.com/in/muhammad-ali-189125119 SUMMARY

• Detail-oriented and highly skilled Laboratory Technician with experience in DNA extraction seeking to contribute expertise and passion for molecular biology to a dynamic research team; commitment to continued learning and professional development in th e field of molecular biology

• Proven track record of executing efficient and accurate DNA extraction protocols, extraction, purification, real-time PCR, Sanger sequencing, and cell culture, ensuring the integrity and purity of isolated DNA for downstream analysis

• Familiarity with laboratory information management systems (LIMS), MS Office Suite to perform data management and analysis, data inputs, data entry, worksheet verification, and result validation

• Experience with safe reagent handling in laboratory settings while prioritizing compliance with safety procedures and following standard operating procedures (SOPs) and Good Laboratory Practice (GLP)

• Strong research and problem-solving skills with the ability to leverage quantitative strengths to solve technical problems, advance scientific breakthroughs, and support innovative research initiatives

• Excellent time management, meticulous organization skills, and superb at multitasking in a fast-paced, deadline-driven environment while still maintaining attention to detail and accuracy EDUCATION

Mohawk College, Hamilton, ON, Canada - Co-op Diploma in Biotechnology (Health) Sep 2022 – Apr 2024

• Graduated with Distinction

• Courses of Interest: Cell Biology and Microbiology Vrije Universiteit Brussel, Brussels, Belgium - Master of Science in Molecular Biology Sep 2020 – Jun 2023

• Graduated with Distinction

• Research Focus: Y Chromosomal Analysis of Plague Victims of Medieval Cambridge International Islamic University Islamabad, Pakistan - Bachelor of Science in Biotechnology Sep 2015 – Jun 2019

• Graduated with Distinction

• Research Focus: Discrimination of Male Individuals by using rapidly Mutating Y-STR Markers PROFESSIONAL EXPERIENCE

Lakehead University, Canada

Internship (DNA Skills Training Course) Jun 2022 – Jul 2022

• Conducted DNA extractions from ancient bones, mummified tissues, buccal swabs, hair, and various blood samples, including blood on rocks and blood on FTA cards

• Amplified mtDNA through qPCR and strengthened proficiency in Sanger sequencing of PCR amplified products Vrije Universiteit Brussel, Brussels, Belgium

Researcher (Final Year Research Project) Sep 2021 – May 2022

• Worked on one of the well-known projects in Archaeogenetics (http://www.aftertheplague.com/)

• Investigated Y-haplogroup’s role in genetic drift, admixtures, and haplogroup retention among modern humans using a DNA dataset from ancient burials in Belgium and Medieval Cambridge

• Involved in creating PCA plots (R-Studio), generating heatmaps (XLStats), and contour maps (ArcGIS)

• Forecasted and planned project requirements with precise communication for on-time and budget completion Laboratory of Genome Engineering, VIB, Belgium

Intern Mar 2021 – May 2022

• Conducted microbiology experiments on bacterial stress response and the Pho-Q/Pho-P two-component system

• Employed Forster resonance energy transfer (FRET) for protein-protein interaction studies

• Designed primers, cloned SlyB and BamA genes into vectors, and confirmed insertions via colony PCR Citi Labs, Pakistan

Lab Assistant Aug 2019 – Aug 2020

• Handled day-to-day operations such as sample collection and handling, DNA extraction and purification, PCR for disease diagnostics, and managing and delivering patient reports

• Documented results on laboratory tests, ensuring proper and accurate data entry; contributed to meeting the departmental/corporate quality metrics, regulatory compliance, SOPs, and quality indicator Dubai Police HQ, United Arab Emirates (UAE)

Research Collaborator Aug 2018 – Jun 2019

• Actively contributed to the development of the Pakistani Rapidly Mutating (RM Y-STR) Haplotype database; played a crucial role in data analysis by applying forensic statistics.

• Assisted in blood sample collection, DNA extraction, purification, and PCR multiplexing of samples Centre for Applied Molecular Biology (CAMB), Pakistan Intern 2017

• Executed lab procedures, including DNA extraction, STR marker amplification, Sanger sequencing, and data analysis

• Performed sample and reagent preparation and responsible for the full compliance of specific standards for product quality following the company's Standard Operating Procedures (SOPs) National Institute of Genomics and Advanced Biotechnology (NIGAB), Pakistan Intern 2016

• Supported team with banana tissue culture, media prep, sucker collection, and ex-plant preparation; gained experience in using autoclaves, thermometers, and pH meters

• Ensured full compliance with specific standards for product quality following the company's Standard Operating Procedures (SO Ps) PUBLICATIONS

Under Review

1. Capturing the fusion of two ancestries and kinship structures in Merovingian Flanders Published

1. Genetic history of Cambridgeshire before and after the Black Death https://www.science.org/doi/10.1126/sciadv.adi5903 2. Medieval social landscape through the genetic history of Cambridgeshire before and after the Black Death https://www.biorxiv.org/content/10.1101/2023.03.03.531048v1 3. Population and Mutational Assessment of Novel Repeats in 13RM-YSTRs an Unrelated Male Born in Gilgit, Pak. Saudi Journal of Biological Sciences May 2022

https://www.sciencedirect.com/science/article/pii/S1319562X22000559 4. Evaluation of RM-YSTRs in Pakistani Population Forensic Science International: Genetics Supplement Series 27th September 2019

https://www.fsigeneticssup.com/article/S1875-1768(19)30048-4/fulltext

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