Resume

ACADEMIC RESEARCH

Location:

San Francisco, CA

Salary:

$100,000/YEAR

Posted:

March 26, 2024

Contact this candidate

Resume:

NHU NGUYEN, MD, PhD

Daly City, CA ***** : 415-***-**** : ad4k8u@r.postjobfree.com

PROFILE

Physician scientist with a strong interest in genetics and septic shock of acute respiratory distress syndrome with 5 years doctoral fellow experience of basic molecular and genetics, and 5 years academic researcher experience in both in vitro and preclinical research.

•Strong experience in molecular biotechnique:

Genomics (DNA and RNA extraction, primer design, Plasmid preparation, ligation, transformation, PCR, site-directed mutagenesis, Gene cloning, qPCR)

Sanger and NGS sequencing (primer design, library prep, DNAseq, RNAseq)

•Screen mutations in human samples from pediatric patients with congenital disorders using Sanger sequencing,

•Design Meta transcriptomic next generation sequencing (mNGS) of E. coli in the rabbit lung microbiome,

•Design an in-house rabbit TCR repertoire profiling method using next generation sequencing (Illumina® MiSeq, NovaSeq),

•Conduct histology and immunohistochemistry and RNAscope in lung and heart rabbit samples.

•Analyze NGS data using R.

Development of cell-bases assays in primary and cultured cells

Mammalian cell work: cell culture, inhibition/proliferation assay, endotoxin

assay, lipofectamine transfection.

Protein biochemistry: prokaryotic overexpression of recombinant proteins (inclusion body/soluble/secreted forms), protein purification by FPLC (affinity, ion exchange, size exclusion chromatography), protein bioconjugation; SDS-PAGE, Western blot, ELISA

•Extensive skill set in designing animal models for preclinical studies (including PK/PD, toxicology, and efficacy studies)

•Strong clinical experience in pediatrics, cardiometabolic disease and inflammatory disease

•Lab management:

Write lab protocols (in vitro, in vivo)/ SOPs,

Review, analyze data (Excel, GraphPad, R, etc.), and write/review manuscripts,

Report and discuss directly to collaborators (CROs)

Supervise students and staffs in hand-on experiments and technical documents.

•Leadership, independent troubleshoot, strong communication and collaboration skills.

Dealing pricing and fees with providers to get 30% discount annually

Negotiating cost-effective contracts with CROs

Design experiments

Overseeing all activities within research team.

RESEARCH EXPERIENCES

University of California San Francisco, School of Medicine, Division of HIV, Infectious Diseases, and Global Medicine, Diep lab - Academic Researcher (2021 – present)

Rabbit Model of Staphylococcus aureus Enterotoxin B (SEB) and SARS-CoV-2 Spike protein Toxic Shock Syndrome

Engaging in translational research aimed at developing and validating new anesthetized, mechanically ventilated rabbit model with advanced hemodynamic parameters using thermodilution catheter and echocardiography as well as longitudinal blood biomarker analyses to:

dissect mechanisms of toxin-induced acute myocardial depression with echocardiography and advanced hemodynamic monitoring with thermodilution cardiac output. This work was also funded by AstraZeneca, presented at the American Thoracic Society conference 2020 and published in Nguyen et al., Frontiers in Immunology 2023.

Ongoing research:

determine whether intravenous infusion or endobronchial instillation with two purified proteins with superantigen activities, Staphylococcus aureus Enterotoxin B (SEB) and SARS-CoV-2 Spike protein, induce toxic shock syndrome,

test whether neutralizing antibodies or active immunization confer protect against intoxication with SEB or Spike,

investigate whether SEB potentiates E. coli bacterial proliferation and lethality by inducing acute myocarditis and myocardial necrosis that serve as a substrate for subsequent ST segment abnormalities, left-ventricular wall asynchronic motion, reduced LV ejection fraction and decreased cardiac output in the rabbit model.

Rabbit Model of Klebsiella pneumoniae Blood Stream Infection

Collaborating with AiCuris Gmbh & Co. to evaluate the new treatment in combating Klebsiella pneumoniae blood stream infection using rabbit models.

University of California San Francisco, School of Medicine, Division of HIV, Infectious Diseases, and Global Medicine, Diep lab - Postdoctoral Researcher (2018 – 2021)

ICU Rabbit Model of Pseudomonas aeruginosa Acute Pneumonia

Achieved in establishing and validating the new rabbit model of acute respiratory distress syndrome that recapitulated not only human ventilator-associated pneumonia due to Pseudomonas aeruginosa but also standardized protocols for ICU-supportive care, including fluid resuscitation, vasopressor, and humanized dosing antibiotic therapy to better mimic clinical practice to test new drugs with (1) description of the natural history of the disease; (2) PK/PD studies in uninfected and infected animals to select the human-equivalent dosing regimen of currently approved drugs; and (3) proof that the infected rabbits can be effectively treated with available antibacterial therapies.

This work was funded by FDA and published in Nguyen et al., Antimicrobial Agents and Chemotherapy 2021.

Rabbit Model of Pseudomonas aeruginosa Acute Pneumonia

Consulting ContracFect Inc., to evaluate the new treatment in combating Pseudomonas aeruginosa Acute Pneumonia using rabbit models.

Rabbit models of S. aureus as a Preclinical Drug Development Tool

Collaborate with Integrated BioTherapeutics to test a multicomponent toxoid vaccine protects against primary and secondary skin infections caused by Staphylococcus aureus in rabbit model. This work is published in Frontiers in Immunology 2021.

Collaborate with MedImmune/AstraZeneca to evidence the protective efficacy of two novel human monoclonal antibodies against staphylococcal bicomponent leucocidins in rabbit model of S. aureus pneumonia and periprosthetic joint infection. This work is published in Nguyen et al., Frontiers in Immunology 2023.

Establish an experimental ICU rabbit model to study the pathophysiology of Staphylococcus aureus septic shock to determine whether a novel immunotherapy can prevent or halt its natural disease progression. This new rabbit model of Staphylococcus aureus septic shock is used to dissect mechanisms of toxin-induced acute myocardial depression with echocardiography and advanced hemodynamic monitoring with thermodilution cardiac output,

Standardize the rabbit model of Staphylococcus aureus septic shock with fluid resuscitation and vasopressor mimicking clinical therapy to determine differences in pathophysiology of infection caused by S. aureus USA300, USA100 and USA500 and their isogenic mutant strains. This model will further be used to determine whether LukED, HlgAB/HlgCB and LukAB induce vascular failure and/or acute myocardial dysfunction. So far, the resuscitated rabbit model will be used for vaccine efficacy studies with monoclonal antibodies.

University of Tokyo, School of International Health, Department of Developmental Medical Sciences – Master & Doctoral Fellow (2013 – 2018)

Genetic analysis of congenital disorders affecting the heart

investigate Ellis-van Creveld (EVC) syndrome, a congenital disorder with debilitating consequences for skeletal, cardiovascular, and craniofacial structures in the human body. This work is published in Nguyen et al., Congenital Anomalies 2016 and 2019.

Clinical and epidemiological characteristics of acute respiratory virus infections in Vietnamese children

Study clinical samples from children with respiratory infections at Children’s Hospital 2, Ho Chi Minh City, Vietnam, which were published in Tran et al., 臨床とウイルス (Clinical Virology) 2014; Tran et al, Epidemiol Infect. 2014 and 2015.

Children’s Hospital 2, Pediatric Cardiologist (2008 – 2013)

Clinical and epidemiological characteristics of rubella infection in Vietnamese children,

The effect of the sildenafil citrate in treating pulmonary arterial hypertension in children with left-to-right shunt congenital heart diseases.

SKILLSETS IN VIVO STUDIES

Design experimental rabbit models

Unanesthetized rabbit models: blood stream infection, acute pneumonia, skin infection, and periprosthetic joint infection,

Anesthetized, mechanically ventilated rabbit model: toxic shock, pneumonia, and ventilator-associated pneumonia.

Train advanced techniques in rabbit models: catheterization (arterial/venous catheters, PICCO catheter), intramuscular injection (IM), intravenous injection (IV), intradermal injection (ID), subcutaneous injection (SC), intravenous medication administration, ear vein blood withdrawal, submental blood collection, cardiac puncture, tissue necropsy, endotracheal intubation, etc.,

Perform echocardiography and electrocardiography (ECG) in rabbit models.

IN VITRO STUDIES

Screen mutations in human samples from pediatric patients with congenital disorders using Sanger sequencing,

Design Meta transcriptomic next generation sequencing (mNGS) of E. coli in the rabbit lung microbiome,

Design an in-house rabbit TCR repertoire profiling method using next generation sequencing (Illumina® MiSeq, NovaSeq),

Perform DNA works including Plasmid preparation, ligation, transformation, PCR, site-directed mutagenesis, Gene cloning,

Perform RNA works including RNA extraction, qRT-PCR, bulk RNAseq,

Isolate and culture PBMC of human and rabbit for binding/killing assay and ELISA/qPCR,

Perform Protein works including recombinant protein production and purification from E. coli, fusion protein technique, protein interaction, western blotting, cytokine ELISA,

Conduct histology and immunohistochemistry and RNAscope in lung and heart rabbit samples.

CLINICAL ACTIVITIES

Perform in diagnosing and treating congenital and cardiovascular diseases in children,

Perform in trans-esophageal echocardiography (pre/post heart operation), fetal echocardiography and cardiac interventional catheterizations.

TEACHING AND MENTORING

Mentor various groups of trainees (students with diverse background, including college student, master students, doctoral students, exchanged students) with different requirements, such as animal experiment, in vitro research, data analysis and scientific presentation in Division of Developmental Medical Sciences, Department of Medicine, University of Tokyo, Japan and Division of HIV, Infectious Diseases, and Global Medicine, Department of Medicine, University of California San Francisco.

EDUCATION

2001-2007 MD, University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam

2008-2011 Residency in Pediatric and Cardiology, Children’s Hospital 2, Ho Chi Minh City

2011-2013 Fellowship in Cardiology, Heart Institute, Ho Chi Minh City, Vietnam

2013-2015 MSc in Medical Sciences, University of Tokyo, Japan

2015-2018 PhD in Medical Sciences, University of Tokyo, Japan

LICENSES, CERTIFICATION

2008Medical license in Pediatric

2009Certificate of Echocardiography & Cardio-pathology

2010Medical license in Neonatology

2013 Certificate of Transesophageal Echocardiography

2018 Certificate of Interventional Cardiology in Pediatrics

2023 California Clinical Genetic Molecular Biologist Scientist Trainee licensure

2023 Certificate of AHA Basic Life Support (CRP and AED)

POSITIONS

2008-2013 Cardiologist, Department of Cardiology, Children’s Hospital 2, Vietnam

2014-2015 Research Assistant, University of Tokyo, Japan

2015-2018 Teaching Assistant, University of Tokyo, Japan

2018-2021 Postdoctoral Researcher, University of California San Francisco, US.

2021 - present Academic Researcher, University of California San Francisco, US.

2023 - present Director of Laboratory Research, Humanity Neurotech Inc, US.

2018 - present Visiting Researcher, University of Tokyo, Japan

HONORS AND AWARDS

2010 Research Development Award

Vietnam-Australia Research Development Conferences, Hanoi, Vietnam

2010 Youth Scientist-Excellent Scientific Report Award

Vietnam-Belgium Pediatrics Conference, Ho Chi Minh City, Vietnam

2013 Postgraduate Scholarship for International Scholar University of Tokyo, Japan

Asian Development Bank – Japan Scholarship Program

2015 Postgraduate Scholarship for International Scholar University of Tokyo, Japan

Tokio Marine Kagami Memorial Foundation

2017 Oral Presentation Award

The 38th Annual Lorne Genome Conference, Victoria, Australia

MEMBERSHIPS

2008 - present Pediatric Association, Ho Chi Minh City, Vietnam

2008 - present Medical Association, Ho Chi Minh City, Vietnam

2014 - 2018 Japanese Pediatric Society, Japan

2019 - present American Thoracic Society, US.

PUBLICATIONS

1.Nguyen TQN, Nguyen VD, Trinh HT. The effect of sildenafil citrate in treating pulmonary arterial hypertension in children with left to right shunt congenital heart disease at Cardiology Department, Children’s Hospital 2, Ho Chi Minh City, Vietnam. Y Hoc TP. Ho Chi Minh (Journal of Medicine Ho Chi Minh City). 2010; 4, 14: 43-47. ISSN: 1859-1779. (https://yhoctphcm.ump.edu.vn/index.php?Content=ChiTietBai&idBai=7674)

2.Tran DN, Nguyen TQN, Okitsu S, Hayakawa S, Mizuguchi M, Ushijima H. Epidemiology of rubella epidemic in Vietnam. 臨床とウイルス (Clinical Virology) 2014; 42(1): 35-46. ISSN: 0303-8092. (https://mol.medicalonline.jp/archive/search?jo=dq0cviro&vo=42&issue=1)

3.Tran DN, Nguyen TQN, Nguyen TA, Hayakawa S, Mizuguchi M, Ushijima H. Human bocavirus in children with acute respiratory infections in Vietnam. J Med Virol. 2014, 86:988994. PMID: 24123072.

4.Tran DN, Trinh DQ, Pham TKN, Vu MP, Ha MT, Nguyen TQN, Okitsu S, Shimizu H, Hayakawa S, Mizuguchi M, Ushijima H. Clinical and epidemiological characteristics of acute respiratory virus infections in Vietnamese Children. Epidemiol Infect. 2015 Jul; 6:1-10. PMID: 26145204.

5.Tran DN, Trinh DQ, Pham TKN, Pham TMH, Ha MT, Nguyen TQN, Okitsu S, Shimizu H, Hayakawa S, Mizuguchi M, Ushijima H. Human rhinovirus infections in hospitalized children: clinical, epidemiological and virological features. Epidemiol Infect. 2015 Jun; 26:1-9. PMID: 26112743.

6.Nguyen TQN, Saitoh M, Trinh HT, Doan NMT, Mizuno Y, Seki M, Sato Y, Ogawa S, Mizuguchi M. Truncation and microdeletion of EVC/EVC2 with missense mutation of EFCAB7 in Ellis-van Creveld syndrome. Congenital Anomalies. 2016; 56, 209-216. PMID: 26748586.

7.Nguyen TQN, Doan NMT, Trinh HT, Mizuguchi M. Novel mutation in EFCAB7 alters expression and interaction of Ellis-van Creveld ciliary proteins. Congenital Anomalies, 2019, 59 (2): 49-50. PMID: 29845660.

8.Vu TTT, Nguyen TQN, Tran VVG, Gras E, Mao Y, Jung DH, Tkaczyk C, Sellman BR, Diep BA. Protective Efficacy of Monoclonal Antibodies Neutralizing Alpha-Hemolysin and Bicomponent Leucocidins in Rabbit Model of Staphylococcus aureus Necrotizing Pneumonia. Antimicrobial Agents and Chemotherapy. 2020; 64(3): e02220-19. PMID: 31844012.

9.Mao Y, Valour F, Nguyen TQN, Doan NMT, Koelkebeck H, Richardson C, Cheng LI, Sellman BR, Tkaczyk C, Diep BA. Multi-mechanistic Monoclonal Antibody Combination Targeting Key Staphylococcus aureus Virulence Determinants in a Rabbit Model of Prosthetic Joint Infection. Antimicrobial Agents and Chemotherapy. 2021; 65(7): e0183220. PMID: 33903108.

10.Karauzum H, Venkatasubramaniam A, Adhikari RP, Kort T, Holtsberg FW, Mukherjee I, Mednikov M, Ortines R, Nguyen TQN, Doan NMT, Diep BA, Lee JC, Aman JM. IBT-V02: a multicomponent toxoid vaccine protects against primary and secondary skin infections caused by Staphylococcus aureus. Frontiers in Immunology. 2021, 12: 624310. PMID: 33777005.

11.Nguyen TQN, Gras E, Tran DN, Nguyen NYN, Lam THH, Weiss WJ, Doan NMT, Diep BA. Pseudomonas aeruginosa Ventilator-Associated Pneumonia Rabbit Model for Preclinical Drug Development. Antimicrobial Agents and Chemotherapy. 2021; 65(7): e0272420. PMID: 33972247.

12.Nguyen TQN, Doan NMT, Sato K, Tkaczyk C, Sellman BR, Diep BA. Monoclonal Antibodies Neutralizing Alpha-Hemolysin, Bicomponent Leukocidins, and Clumping Factor A Protected Against Staphylococcus aureus-induced Acute Circulatory Failure in a Mechanically Ventilated Rabbit Model. Frontiers in Immunology Inflammation. 2023, 14:1260627. DOI 10.3389/fimmu.2023.1260627.

13.Gras E, Vu TTT, Nguyen TQN, Tran VVG, Mao Y, Tran DN, Mai HN, Dong OX, Jung DH, P. Iorio NLP, Povoa HCC, Pinheiro MG, Aguiar-Alves F, Weiss WJ, Zheng B, Cheng LI, Stover CK, Sellman BR, DiGiandomenico A, Gibault L, Valour F, Diep, BA. Development and Validation of a Rabbit Model of Pseudomonas aeruginosa Non-Ventilated Pneumonia for Preclinical Drug Development. Frontiers in Cellular and Infection Microbiology. 2023, 13:1297281. DOI 10.3389/fcimb.2023.1297281.

REVIEW ARTICLES

1.NCOMMS-21-20973: "Inhibition of a Lung Microbiota-derived Peptide Ameliorates Acute Exacerbation of Pulmonary Fibrosis". Nature Communications. July 2021.

2.Spectrum00808-21: "Sub-inhibitory concentrations of oxacillin, but not clindamycin, linezolid, or tigecycline, decrease staphylococcal Phenol-soluble modulin expression in community-acquired methicillin-resistant Staphylococcus aureus.". Microbiology Spectrum. August 2021.

3.Spectrum00808-21R1: "Sub-inhibitory concentrations of oxacillin, but not clindamycin, linezolid, or tigecycline, decrease staphylococcal Phenol-soluble modulin expression in community-acquired methicillin-resistant Staphylococcus aureus.". Microbiology Spectrum. November 2021.

4.Research Article 6339358: "Prevalence, Predictor, and Impact of Spontaneous Reperfusion in Patients with ST segment Elevation Myocardial Infarction: Two-year Follow-up Study from a Single Center Registry". Journal of Interventional Cardiology. December 2021.

5.NCOMMS-21-20973A: "Inhibition of a Lung Microbiota-derived Peptide Ameliorates Acute Exacerbation of Pulmonary Fibrosis". Nature Communications. January 2022.

6.AAC02298-21: “The azithromycin pro-drug CSY5669 boosts bacterial killing while attenuating lung inflammation associated with pneumonia caused by methicillin-resistant Staphylococcus aureus". Antimicrobial Agents and Chemotherapy. January 2022.

7.Spectrum02181-21R1: “Genomics of Staphyloccocus aureus and Staphylococcus epidermidis from periprosthetic joint infections and correlation to clinical outcome”. Microbiology Spectrum. April 2022.

8.Research Article 904189: “Enhancement of rubella virus infection in immortalized human first trimester trophoblasts under low glucose stress conditions". Frontiers in Microbiology. May 2022.

9.AAC02298-21R1: “The azithromycin pro-drug CSY5669 boosts bacterial killing while attenuating lung inflammation associated with pneumonia caused by methicillin-resistant Staphylococcus aureus". Antimicrobial Agents and Chemotherapy. July 2022.

10.Scholar-2023-0080OT: “Febrile Neutropenia: On the Fly”. ATS Scholar. September 2023.

CONFERENCE

1.Nguyen TQN, Nguyen VD, Trinh HT. The effect of sildenafil citrate in treating pulmonary arterial hypertension in children with left to right shunting congenital heart disease at Department of Cardiology, Children’s Hospital 2, Ho Chi Minh City, Vietnam. Vietnam-

Australia Research Development Conference, November 7th-8th 2010, in Ha Noi, Vietnam.

2.Nguyen TQN, Nguyen VD, Trinh HT. The treatment of pulmonary arterial hypertension in children with left to right shunting congenital heart disease at Cardiology Department, Children’s Hospital 2, Ho Chi Minh City, Vietnam. Vietnam-Belgium Pediatrics Conference, December 15th-16th 2010, in Ho Chi Minh City, Vietnam.

3.Nguyen TQN, Nguyen MTV, Trinh HT, Nguyen VD. The diagnosis and treatment of refractory pulmonary hypertension in children with congenital heart disease. Vietnam national Congress of Cardiovascular and Thoracic Surgery, December 10th-11th 2010, in Hue City, Vietnam.

4.Tran DN, Nguyen TQN, Hayakawa S, Mizuguchi M, Ushijima H. Molecular characteristics of human influenza A viruses in Vietnam from 2010 to 2011. The 13th St. Jude/PIDS Pediatric Infectious Diseases Research Conference. February 21st-22nd 2014, in Memphis, TN, US.

5.Nguyen TQN, Saitoh S, Trinh HT, Mizuguchi M. Novel pathogenic mutations in Ellis-van Creveld syndrome. The 11th Asian Society for Pediatric Research congress. April 15th18th 2015, in Osaka, Japan.

6.Nguyen TQN, Saitoh S, Trinh HT, Doan NMT, Mizuno Y, Seki M, Sato Y, Ogawa S, Mizuguchi M. Truncation and microdeletion of EVC accompanied by a novel EFCAB7 missense mutation in Ellis-Van Creveld syndrome with atypical congenital heart defect. The 4th International Conference on Pediatrics & Pediatric Emergency Medicine, Mar 29th-31st 2016, in Atlanta, Georgia, U.S.

7.Nguyen TQN, Saitoh S, Doan NMT, Trinh HT, Mizuguchi M. Ellis-van Creveld syndrome: Mutation in EFCAB7 changes the EFCAB7-EVC2 interaction in Hedgehog signaling regulation. The 38th Lorne Genome Conference 2017, Feb 12th-14th 2017, in Mantra Lorne, Victoria, Australia.

8.Nguyen TQN, Doan NMT, Tran DN, Mai HN, Vu TTT, Tkaczyk C, Sellman BR, Diep BA. Toxin Neutralization Therapy Protected Against Staphylococcal Septic Shock - Results from an Experimental Intensive Care Unit Using Outbred Rabbits. American Thoracic Society 2020, May 2020, in Philadelphia, U.S.

9.Doan NMT, Nguyen TQN, Mai HN, Tran DN, Warrener B, Sellman BR, DiGiandomenico A, Diep BA. Pseudomonas aeruginosa Type III Secretion System, but not Exotoxin A, Causes Acute Respiratory Distress Syndrome Using a Rabbit Ventilator-Associated Pneumonia Model. American Thoracic Society 2020, May 2021, in San Diego, U.S.

https://scholar.google.com/citations?hl=en&user=lsSlQ3EAAAAJ https://orcid.org/my-orcid?orcid=0000-0002-2203-950X

REFERENCE

Binh DIEP, PhD

Professor

Division of HIV, Infectious Diseases, and Global Medicine

University of California, San Francisco 1001 Potrero Ave, Building 30, Room 3318 San Francisco CA 94110, US.

Tel: +1-408-***-****

Fax: +1-415-***-****

Email: ad4k8u@r.postjobfree.com

Christine TKACZYK, PhD

Director

Early Vaccines & Immune Therapies