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SHIN-YI DU, PHD

• ad4jw7@r.postjobfree.com • 857-***-**** • 60 Fox Run Drive, Plainsboro, New Jersey SUMMARY

Experienced, resourceful and knowledgeable Scientist highly regarded for a molecular biology & immunology background within biomedical research & assay development. Motivational leader and collaborator who guides high- performing team in achieving significant projects. Excellent communication skills and strong ability to work in matrix environments. Extensive interests in understanding the mechanism of human diseases and making more contributions to improve human health.

EXPERTISE/SKILLS

Techniques:

DNA/RNA/micoRNA extraction, qRT-PCR, ddPCR, plasmid construction and isolation, sequencing, restriction enzyme digestion, cloning, primary cell culture, PBMC isolation and culture, T cell expansion, cell banking, cell proliferation assays, cell-based immunoassays, siRNA transfection, protein purification, SDS-PAGE, Western blotting, ELISA, ELISPOT, MSD, multi-color flow cytometry, co-immunoprecipitation, NGS, HPLC, UV/Vis, luciferase assay, immunohistochemistry staining, fluorescence microscopy, confocal microscopy Technical Skills:

MS Word, Excel, PowerPoint, Outlook, GraphPad Prism, ImageJ, Softmax Pro, FlowJo, UniProt, Inkscape, Oligo, BLAST, PDB, VMD, KEGG, BIOVIA Notebook, LabWare LIMS PROFESSIONAL EXPERIENCE

Scientist II, Analytical Development (2022 – 2023) PTC Therapeutics, Inc. Hopewell, NJ

• Developed and validated cell-based and molecular assays for potency and infectivity with a variety of readouts.

• Conducted methods improvement by regularly analyzing the data trends and troubleshooting the bottlenecks.

• Led the complete transfer and set up of MSD & ELISA assays for AAV gene therapy projects.

• Collaborated with cross-functional teams to deliver the data to QC & PD group for qualification of the assays.

• Authored internal technical reports/SOPs and coordinated internal & external analytical method transfer activities.

• Experienced in engaging with Contract Development and method transfer to CRO/CDMOs.

• Maintained, reviewed, and approved ELNs to ensure strict compliance with GxP requirement.

• Supervised and improved junior scientists’ deliverables. Research Scientist, Analytical Development (2021 – 2022) Elucida Oncology, Inc. Monument Junction, NJ

• Developed, optimized, and troubleshooted analytical methods to support formulation and stability assessment.

• Collaborated with technical teams outside the company to analyze and characterize samples of process development by working with techniques such as qPCR, Western blots, NGS, flow cytometry, and confocal microscopy.

• Supported all areas of the development process, including design, development, transfer, qualification, and validation.

• Set up analytical development laboratory and laboratorial expansion by collaborating with different teams.

• Performed evaluations of new testing protocols and revising testing procedures for in vitro prodrug release studies.

• Conducted assessment of the IND/BLA filings in compliance with FDA regulatory and pharmacopeial guidelines.

• Supervised and trained technical staff.

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Postdoctoral Fellow (2019 – 2021)

Northwell Health - Feinstein Institutes for medical research Manhasset, NY

• Participated in multiple research projects involving immunological and molecular biological investigations into how immune cells are activated in the host response to autoimmune disorders.

• Prepared and independently executed experimental work plans.

• Studied immunological mechanisms activated in the progression of chronic inflammatory diseases.

• Developed a functional cell-based assay to classify leukocyte-associated Ig-like receptor 1 related anti- inflammatory mechanism in human monocytes.

• Managed laboratory supplies and equipment and negotiated with vendors for purchase and repairs.

• Mentored junior graduate students and trained laboratory technicians. Research Fellow (2016 – 2018)

Memorial Sloan Kettering Cancer Center New York, NY

• Conducted experiments on several projects and collaborations, optimized and troubleshooted protocols and methods to reduce time and costs.

• Prepared and addressed reviewers’ comments on paper publishment to assure project success in a timely manner.

• Optimized the detection methods for dehydroepiandrosterone sulfate measurement in prostate cancer cell lines.

• Identified the novel biomarkers and therapeutic targets of castration resistant prostate cancer.

• Participated in the training of new scientists.

Postdoctoral Fellow (2014 – 2016)

National Health Research Institutes Miaoli, Taiwan

• Defined research approaches, designed experimental schemes and participated in the analyzes of the results during the course of published research in peer-reviewed journal articles.

• Solved experimental problems by developing novel assays using cellular and animal models.

• Established 3D organotypic human skin equivalent for investigating potential health impacts of infrared light on skin and immune system.

• Assisted National Science Council grant writing and application. EDUCATION

Doctor of Philosophy in Molecular and Cellular Biology (2014) National Tsing Hua University Hsinchu, Taiwan

Dissertation: Helicobacter pylori cholesterol-a-glucosyltransferase confers delayed phagocytosis by macrophages

• Awards/Honor: The National Science Council Travel Award of Taiwan Bachelor of Science in Zoology (2002)

National Chung Hsing University Taichung, Taiwan

• Awards/Honor: Member of the Phi Tao Phi Scholastic Honor Society of Taiwan SELECTED PUBLICATIONS

• Peng T, Du S-Y, Son M and Diamond B. (2021) HIF-1alpha is a negative regulator of interferon regulatory factors: Implications for interferon production by hypoxic monocytes. PNAS. 118(26):1-10

• Sun T*, Du S-Y*, Armenia J*, Qu F, Fan J, Wang X, Fei T, Komura K, Liu S. X., Lee G-S M. and Kantoff P. W. (2018). Expression of the LncRNA MIR222HG co-transcribed from the miR-221/222 gene promotor facilitates the development of castration resistance prostate cancer. Oncogenesis. 7(3):30-42

• Du S-Y*, Wang H-J*, Cheng H-H, Chen S-D, Wang H-C, and Wang W-C. (2016) Cholesterol glucosylation by Helicobacter pylori delay internalization and arrests phagosome maturation in macrophages. Journal of Microbiology, Immunology and Infection. 49(5): 636-45

• Lai C-H, Chang Y-C, Du S-Y, Wang HJ-, Kuo C-H, Fang S-H, Fu H-W, Lin H-H, Chiang A-S, and Wang W- C. (2008) Cholesterol depletion reduces Helicobacter pylori CagA translocation and CagA-induced responses in AGS cells. Infect Immun. 76: 3293-3303

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