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Ariana Mostafa

**** ********* *****, ** *****, CA 92037

ad3xou@r.postjobfree.com

248-***-****

PROFESSIONAL SUMMARY:

Highly analytical and skilled scientist with extensive research and development background in molecular biology, detection assays such as PCR and LAMP, as well as Crispr based technology. Practiced in NGS sequencing analytics and basic computer programming such as Matlab/Python. Looking to utilize professional experience, technical expertise, and innovative to grow in scientific industry. SKILLS:

• Assay Development

• Experimental Design

• Laboratory Techniques

• Quality Control

• Data and Technical Analytics

• Statistical Analysis

• Next Generation Sequencing

• RNA Sequencing

• Python/R programming

• MATLAB

• Microfluidic devices and fabrication

WORK EXPERIENCE:

Scientist at VedaBio, Inc. (Formerly Labsimply, Inc.) May 2021 – Present

• Led Crispr-based assay/product development and in-vitro diagnostics (IVD), fostering innovation in the field of molecular biology

• Designed and executed experiments to characterize the performance characteristics of diagnostic assays including sensitivity, specificity, dynamic range, limit of detection using both synthetic and contrived samples.

• Conducted enzyme characterizations to evaluate the functionality and properties of enzymes, including enzyme kinetics, substrate specificity, and stability under varying conditions.

• Utilized MATLAB, Python, R, and Jupyter notebook based bioinformatics tools for data analysis and assay optimization.

• Utilized Short-Read Amplicon sequencing and small RNA sequencing to analyze indels in target sequences.

• Mentored research associates, fostering scientific excellence and continuous learning.

• Contributed to intellectual property development with multiple patents related to the novel technology.

• Collaborated with cross-functional teams, including product development, quality control, application development, and regulatory affairs, to support product commercialization efforts and ensure compliance with regulatory requirements and quality standards.

Research Assistant at University of Illinois Urbana-Champaign August 2017 – May 2021

• Conducted cutting-edge research in nucleic acid-based amplification (PCR and LAMP) and assay development for point-of-care and lab-based diagnostic platforms for detection of pathogens in crude samples such as blood, nasopharyngeal swabs and saliva, and meat/food matrices.

• Worked on Linux systems for mathematical modelling of isothermal amplification in our novel “Biphasic” amplification method.

• Collaborated on integrating microfluidic cartridge-based systems for point-of-care applications.

• Analyzed experimental data using MATLAB and Prism, generating insights into assay performance.

• Developed and optimized microchip-based organoid culture platforms, enabling the growth and maintenance of three- dimensional tissue models for disease modeling and drug screening applications.

• Utilized mate pair sequencing to study tumor evolution of organoids.

• Skilled in design and fabrication of microfluidic devices, including microchips and microfluidic cartridges, utilizing techniques such as photolithography, wet etching, and dry etching.

• Presented research findings at national conferences, authored several scientific publications in peer-reviewed journals, and assisted in grant writing and proposal development Teaching Assistant at University of Illinois Urbana-Champaign August 2017-May 2018

• Facilitate student understanding of fundamental concepts in physiology.

• Assisted in delivering course content and providing clarification on complex topics

• Led hands-on laboratory sessions, guiding students through experimental procedures related to physiological principles.

• Supervised and mentored students during lab sessions, offering individualized support and feedback.

• Implemented innovative teaching strategies and instructional materials to enhance student engagement

• Evaluated student performance through the assessment of lab reports, quizzes, and assignments, providing constructive feedback and guidance.

2

Kumon Math and Reading Center, Senior Instruction/Center Assistant 2007-2017

• Instructed students aged 3 to 16 in personalized learning programs math and reading.

• Provided individualized instruction tailored to each student's learning pace and style,

• Developed engaging lesson plans and learning activities to reinforce key concepts

• Monitored student progress through regular assessments and evaluations

• Collaborated with parents to communicate student progress and learning goals

• Assisted in administrative tasks and center operations. EDUCATION:

UNIVERSITY OF ILLINOIS URBANA-CHAMPAIGN August 2017-May 2021 PhD, Bioengineering

• Thesis: High Sensitivity Pathogen Detection Assays from Crude Samples Master of Science, Bioengineering December 2019

WAYNE STATE UNIVERSITY

Bachelor of Science, Biomedical Engineering May 2015 PUBLICATIONS:

Shen, M; Mostafa, A; Begeman, P.C.; Saif T.; Yang, K. H. Study of Phalangeal Fracture Threshold and Design of a Surrogate for Human Finger. Orthopaedic Research Society.

Mostafa, A., Ganguli, A., Berger, J., Rayabharam, A., Saavedra, C., Aluru, N. R., & Bashir, R. (2021). Culture-free biphasic approach for sensitive detection of Escherichia coli O157:H7 from beef samples. Biotechnology and Bioengineering, 118, 4516– 4529 Ganguli, A., Lim, J., Mostafa, A., et al. (2022). A culture-free biphasic approach for sensitive and rapid detection of pathogens in dried whole- blood matrix. Proceedings of the National Academy of Sciences of the United States of America, 119(40), e2209607119. https://doi.org/10.1073/pnas.220-***-****

Ganguli, A., Mostafa, A., Berger, J., Aydin, M. Y., Sun, F., Ramirez, S., Valera, E., Cunningham, B. T., King, W. P., & Bashir, R. (2020). Rapid isothermal amplification and portable detection system for SARS-CoV-2. Proceedings of the National Academy of Sciences of the United States of America, 117(37), 22727–22735. https://doi.org/10.1073/pnas.201-***-**** Ganguli, A., Mostafa, A., Berger, J., Lim, J., Araud, E., Baek, J., Stewart de Ramirez, S. A., Baltaji, A., Roth, K., Aamir, M., Aedma, S., Mady, M., Mahajan, P., Sathe, S., Johnson, M., White, K., Kumar, J., Valera, E., & Bashir, R. (2021). Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Ultrasensitive Detection of SARS-CoV-2 in Saliva and Viral Transport Medium Clinical Samples. Analytical chemistry, 93(22), 7797–7807. https://doi.org/10.1021/acs.analchem.0c05170 Ganguli, A., Mostafa, A., Saavedra, C., Kim, Y., Le, P., Faramarzi, V., Feathers, R. W., Berger, J., Ramos-Cruz, K. P., Adeniba, O., Diaz, G., Drnevich, J., Wright, C. L., Hernandez, A. G., Lin, W., Smith, A. M., Kosari, F., Vasmatzis, G., Anastasiadis, P. Z., & Bashir, R. (2021). Three- dimensional microscale hanging drop arrays with geometric control for drug screening and live tissue imaging. Science advances, 7(17), eabc1323. https://doi.org/10.1126/sciadv.abc1323

Ganguli, A., Faramarzi, V., Mostafa, A., Hwang, M.T., You, S., Bashir. R. High Sensitivity Graphene Field Effect Transistor Based Detection of DNA Amplification. Adv. Funct. Mater. 2001031 (2020) doi:10.1002/adfm.202001031. PATENTS

1. Signal Boost Cascade Assay

• Patent Number: 11884921

• Filed: December 9, 2022

• Date of Patent: January 30, 2024

• Assignee: VedaBio, Inc.

• Inventors: Anurup Ganguli, Ashish Pandey, Ariana Mostafa, Jacob Berger 2. Tuning Cascade Assay Kinetics via Molecular Design

• Patent Number: 11884922

• Filed: June 10, 2023

• Date of Patent: January 30, 2024

• Assignee: VedaBio, Inc.

• Inventors: Ariana Mostafa, Jacob Berger, Ashish Pandey, Anurup Ganguli 3. Tuning Cascade Assay Kinetics via Molecular Design 3

• Patent Number: 11859182

• Filed: May 31, 2023

• Date of Patent: January 2, 2024

• Assignee: VedaBio, Inc.

• Inventors: Anurup Ganguli, Ariana Mostafa, Jacob Berger, Ashish Pandey 4. Tuning Cascade Assay Kinetics via Molecular Design

• Patent Number: 11820983

• Filed: December 6, 2022

• Date of Patent: November 21, 2023

• Assignee: LabSimply, Inc.

• Inventors: Anurup Ganguli, Ariana Mostafa, Jacob Berger, Ashish Pandey 5. Compositions of Matter for Detection Assays

• Patent Number: 11702686

• Filed: July 9, 2022

• Date of Patent: July 18, 2023

• Assignee: LabSimply, Inc., The Board of Trustees of the University of Illinois

• Inventors: Anurup Ganguli, Ariana Mostafa, Jacob Berger, Ashish Pandey, Rashid Bashir 6. Compositions of Matter for Detection Assays

• Patent Number: 11639520

• Filed: July 9, 2022

• Date of Patent: May 2, 2023

• Assignee: LabSimply, Inc.

• Inventors: Anurup Ganguli, Ariana Mostafa, Jacob Berger, Ashish Pandey, Rashid Bashir 7. Tuning Cascade Assay Kinetics via Molecular Design

• Grant Number: 11884922

• Filed: June 10, 2023

• Date of Grant: January 30, 2024

• Assignee: VedaBio, Inc.

• Principal Investigators: Ariana Mostafa, Jacob Berger, Ashish Pandey, Anurup Ganguli 8. Tuning Cascade Assay Kinetics via Molecular Design

• Grant Number: 11859182

• Filed: May 31, 2023

• Date of Grant: January 2, 2024

• Assignee: VedaBio, Inc.

• Principal Investigators: Anurup Ganguli, Ariana Mostafa, Jacob Berger, Ashish Pandey 9. Tuning Cascade Assay Kinetics via Molecular Design

• Grant Number: 11820983

• Filed: December 6, 2022

• Date of Grant: November 21, 2023

• Assignee: LabSimply, Inc.

• Principal Investigators: Anurup Ganguli, Ariana Mostafa, Jacob Berger, Ashish Pandey 10. Compositions of Matter for Detection Assays

• Grant Number: 11702686

• Filed: July 9, 2022

• Date of Grant: July 18, 2023

• Assignee: LabSimply, Inc., The Board of Trustees of the University of Illinois

• Principal Investigators: Anurup Ganguli, Ariana Mostafa, Jacob Berger, Ashish Pandey, Rashid Bashir 11. Compositions of Matter for Detection Assays

• Grant Number: 11639520

• Filed: July 9, 2022

• Date of Grant: May 2, 2023

• Assignee: LabSimply, Inc.

• Principal Investigators: Anurup Ganguli, Ariana Mostafa, Jacob Berger, Ashish Pandey, Rashid Bashir

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