TIMOTHY HUNTER

** *. ******* ****, *-**

Ridley Park, PA 19078

ad3d6k@r.postjobfree.com

484-***-****

EDUCATION

Binghamton University, Binghamton, NY

PhD. Biology, Completed One Year.

August 2021 – May 2022

Villanova University, Villanova, PA

M.S, Biology, December 2021

Temple University, Philadelphia, PA

B.S. Biology, May 2018

Dean’s List - Fall 2016, Spring 2017, Fall 2017

RESEARCH EXPERIENCE

Binghamton University

Graduate Student

Biology Department

ITC Complex

Jeffery Schertzer

August 2021 to May 2022

Examined the role of outer membrane-vesicles in drug delivery and biofilm development in Pseudomonas aeruginosa. Also, examined the roll of the molecule PQS on the development of outer-membrane vesicles in Pseudomonas and other gram-negative bacteria.

Graduate Student

Biology Department

Mendel Science Center, Lab G13

Villanova University

James W. Wilson, Ph.D.

August 2018-May 2021

Cloned the pdu operon which encodes for the PDU Microcompartment that breaks down the sugar 1,2-PD in different Salmonella enterica Serovar and transferred it to other gram-negative bacteria. Gram-negatives containing the operon were examined for their ability to produce functional PDU MCPs and utilize 1,2-PD.

Undergraduate Lab Assistant

Temple University

Lewis Katz School of Medicine

Kresge Hall, Room 505

Bettina Buttaro, Ph.D.

December 2016-May 2018

Examined the effects of the bacterial amyloid curli on biofilm rigidity Salmonella enterica Typhimurium. Also, examined biofilm dynamics in Enterococcus faecalis and Streptococcus mutants to help understand genetic transfer in the gut microbiome.

PUBLISHED WORKS

The Journal of Bacteriology: Development of a new bead movement based computational framework shows bacterial amyloid curli reduces bead mobility in biofilms. Published in August 2020.

LABORATORY SKILLS

•Knowledge of microbiological techniques including preparation of bacterial growth medium, microbial plating, staining of microbial organisms, aseptic culture technique, bacterial conjugation and bacterial competition assays.

•Demonstrated use of laboratory equipment such as pipettes, centrifuge, and scanning confocal microscope.

•Knowledge of multiple cell biology techniques such as culturing mouse cell lines, splitting cells, injecting mice with cells, and transfection of DNA into mouse cells.

•Knowledge of multiple molecular biology techniques including DNA extraction, RNA extraction, PCR, protein extraction, DNA and protein gel electrophoresis, Western blotting and ELISA.

WORK EXPERIENCE

Quality Assurance Microbiologist

Veltek Associates Inc.

Malvern, PA

September 2022-Present

Quality Assurance Microbiologist for Veltek Associates Inc. Responsibilities include working in a GMP lab setting, conducting personnel and environmental monitoring for the presence of microbes to assure products remain sterile, counting bacterial colonies from environmental monitoring to determine CFUs, streaking bacterial isolates for identification, perform quality control on production and lab equipment

Teaching Assistant

Binghamton University

Biology Department

August 2021-May 2022

Teaching assistant for General Biology and General Microbiology. Responsibilities include teaching students, grading, helping students in lab, preparing media, cultures and reagents for lab classes.

Teaching Assistant

Villanova University

Biology Department

August 2018-June 2021

Teaching assistant for General Biology, General Microbiology, Pharmacology, and Bacteriology. Responsibilities included teaching students, grading, helping students in lab, preparing media, cultures and reagents for lab classes.

Wistar Institute

3601 Spruce Street

Philadelphia, PA

Summer 2018

Assisted with experiments to examine Ebola virus DNA vaccines.

Commonwealth National Golf Club

Horsham, PA

Summers - 2014-2017 and holidays and school breaks

Pet Care Specialist

Petsmart

North Wales, PA

Summer 2016

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