Research Associate Engineering Lab

Curriculum Vitae

Razi Ullah

Research Associate Fellow,

Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Lab for Vascular Implants College of Bioengineering Chongqing University, Chongqing 400030, China

Date of Birth: 04 April, 1989

Email: *********@*****.***

Cell: +92-307*******/+92-344*******

Research Interest & Objectives

My research interest is focused on molecular epidemiology of different parasitic, bacterial and viral diseases as well as identification and molecular characterization of potent antigenic biomarkers for the development of diagnostic assay such as ELISA and In-silico vaccine production. In-line with my research interest I have published research and review articles in peer reviewed well known journals. My current research on gut microbiome analysis of dengue transmission vector Aedes agypti and Aedes albopictus and genetic transformation of their gut microbiota as well as some other related projects on the gut microbiome continues. Research keywords includes Echinococosis, Rotavirus, MRSA, Dengue virus, SARS-Corona virus 2, Vector control, Gut Microbiome, Plasma Microbiome, HCC, CVD, Diagnostic Biomarkers, Antigenic peptides, Antibiotic Resistance, Conjugation and Vaccinomics. Academics

Ph.D Biomedical

Engineering and

Microbiology

2019-2023 (College of Bioengineering Chongqing University, Chongqing 400030)

M.Phil Medical

Microbiology

2017 (Khyber Medical University, Peshawar, Pakistan) BS Microbiology 2015 (Hazara University Mansehra, KPK,University, Pakistan)

Work Experience

1. Research Associate at Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Lab for Vascular Implants College of Bioengineering Chongqing University, Chongqing 400030, China during PhD project.

2. Research Associate & Lab Manager at Molecular Virology Laboratory, Department of Biosciences, Khyber Medical University Peshawar in the project entitled “isolation and Identifications of “super bug bacteria” from the wound of diabetic patients through gene sequencing, since December, 2014 to 2017. 3. Research Associate & Lab Manager at Abdul Wali Khan University Department of Microbiology Garden Campus Mardan KPK Pakistan (2017-2018)

4. District Surveillance Officer at World Health Organization (WHO), The key work was the epidemiological survey of the population for the screening of different diseases and providing of proper prevention and control strategies by educating the population.

5. Lecture (Visiting) at Department of Allied Health Sciences, COMWAVE Institute (DEC) Sarhad University Peshawar), Islamabad, Pakistan since 2017.

6. Internee at Provincial Food Analysis Laboratory, Hayatabad, Peshawar, KP, Pakistan (09, May, 2016 to 09, May2017).

Laboratory Skills

Have a command to perform various microbiological and biochemical test, Equipment which I have used frequently, Anaerobic Bugbox, Physical/Digital Balance, PCR, qPCR, Basic of HPLC, Microscopy, Spectroscopy and; handling of ApoE Mice and C57JBL6 mice as disease model animals. Publications (Recent first)

1. Ullah, R.;Khan, J.; Basharat, N.; Huo, D.; Din, A.U.; Wang, G. Evaluation of Cardiac Biomarkers and Expression Analysis of IL-1, IL-6, IL-10, IL-17, and IL-25 among COVID-19 Patients from Pakistan. Viruses

(2022) In Proofread (IF= 5.818).

2. Wasim, M., Khan, A., Biland, B., Waqar, M., Akbar, N., Ullah, R et al. (2014). Prevalence of Active HCV Infection and Identification of its common Genotypes in District Mardan, Pakistan. International Research Journal of Medical Sciences. 2(4), 7-11:ISSN 2320-7353. (Available at: www.isca.in, www.isca.me).

(IF=3.24).

3. Rehman, H., Wasim, M., Waqar, M., Khan, A., Idrees, M., Ismail, Z., Ali, A., Faisal. Khan, J., Javed, U., Saleem, M., Ahmad, A., Ullah, R et al. (2014). Seroprevalence of HBV Infection and Viral Loads in Outpatients attending a district hospital located in Mardan, Pakistan. 4(5) 28-34. International Journal of Biosciences [IJB]. ISSN: 2220- 6655 (print) 2222-5234 (online) at http://dx.doi.org/10.12692/ijb/4.5.28-34.

(IF= 2.113)

4. Ahmad, T., Ahmad, K., Naseerullah., Gohar, J., Raziullah., Khan F., Khan, S., Muhammad, R et al. (2013). A Short Report on Tuberculosis in District Dir (Lower) Khyber Pakhtunkhwa Pakistan. 1(30) DOI: 10.9780/2321- 3485/1302013/73. (IF= 2.043)

5. Muhammad, M., Biland, B., Idrees, M., Zeb, M., Waqar, M., Khan, M., Ali, S., Afridi, M., Shareen, M., Ullah, R., Ahmad, W., Faisal, S., Saif, I., Rehman, S. et al (2014). Assessment of Risk Factors and Clinical Presentation in a Liver Cirrhotic StatePakistan. World Applied Science Journal 32(7): 1252-1257 DOI: 105829/idosi.wasj.2014.32.07.8672. (IF= 3.714)

6. Impact of Single Nucleotide Polymorphism (SNPs) on ACE-I, ACE-II, TMPRSS2, and IFITM3 expression among COVID-19 patients from Pakistan. (SUBMITTED) 7. Despite the Genetic Variability; NS1 of Different Dengue Serotypes has Comparable Affinity for Various Host Protein in Silico” (In Pipeline)

8. Epidemiological and Molecular Characterization of aminoglycosides and B-lactam resistance genes among clinical isolates of Urinary Tract Infections of Tertiary care hospital, Islamabad, Pakistan (In Pipeline) 9. Clinical and Bacteriological Investigation of Diabetic Foot Infections in Pakistan (In Pipeline) Management and Organization Skills

1. Managing Molecular Virology Laboratory and facilitating the entire research students in research as well as the dealing with distributors of various chemicals and instruments used in research. 2. Organized five (05) days Hands-on Training workshop on “Molecular vs Dynamic Control and Coordinating Development in Vector Control for Prevention of Dengue Viral Infection” (24-28 January, 2022) held at COMSATS University Islamabad, Pakistan

3. Organized Two (02) days International Conference on “Changing Climate and Dengue Fever In The Era of COVID-19” on 09-10 February, 2022 at COMSATS University Islamabad, Pakistan. Laboratory Techniques Earned

Maintenance of Microbial Culture

Diagnosis of Microbial diseases

Microbial Culture Media Preparation

Microbial Culture Sensitivity assay

ELISA, Microscopy, Staining

Molecular Characterization of Antibiotic Resistance Genes

SDS-PAGE Analysis, Dot ELISA development

DNA and RNA extraction and cDNA Preparation

Conventional PCR, Gradient PCR and PCR-RFLP

Gel Electrophoresis

Bioinformatics/Computer Competency

One-way Annova, Chi-square test and Regression analysis through SPSS

Insilico Vaccine Development and Phylogenetic tree development

Primer Designing and Blast Analysis

MS Office (Word, Excel, Powerpoint)

UCSC Genome browser

Ensemble Genome browser

Genomic and Proteomic blast analysis

CLC Main Workbench

Molecular Docking

Molecular Evolutionary Genetics Analysis software Awards/Achievements

Winner of Chief Minister Laptop Top 2013

Winner of Prime Minister Laptop 2017

Winner of Ph.D Fellowship at CQU

Winner of Prime Minister Fee Reimbursement Scheme of M.Phil.

Received a talent Certificate from Prime minister of Pakistan.

Previous internee of Prime Minister National Internship Program (NIP).

Silver Medal won in BSc (Hon) as a 2nd position holder in department and in class.

Researcher Award of Department of Microbiology, presented by Study Aid Foundation for Excellence in 2015

Trainings/ Workshops

Five (05) days Hands-on Training workshop on “Molecular vs Dynamic Control and Coordinating Development in Vector Control for Prevention of Dengue Viral Infection” (24-28 January, 2022) held at COMSATS University Islamabad, Pakistan.

Two days Bio-safety Training (August, 2016) Held at Quaid-i-Azam University Islamabad-Pakistan

Two days GIS Training (Dec, 2016) Held at Quaid-i-Azam University Islamabad-Pakistan

1st International Symposium on The Himalayas of Pakistan Resources and Conservation Issues dated 27-30 November 2014 Organized by Department of Genetics, Hazara University Mansehra held at Hazara University Mansehra Khyber Pakhtunkhwa Pakistan

Training workshop on Research Planning, Data Analysis, Report Writing and Presentation Skills for “Biosciences” organized by Department of Botany, Hazara University Mansehra Pakistan dated 4-5 December, 2015.

Two week 32nd Post graduate training workshop “Uses of nuclear and other techniques in food and agriculture at NIFA, Peshawar dated 19-30 September 2016.

7th Annual Health Research Conference April 29-30 and May 2016 Organized by Office of Research Innovation and Commercialization (ORIC).

Conference and Seminars

Two (02) days International Conference on “Changing Climate and Dengue Fever In The Era of COVID-19” as a speaker and participant on (09 & 10 February, 2022) held at COMSATS University Islamabad, Pakistan.

01 day Seminar on “Nanotechnology in Microbiology and Biofuel” held on (12 February, 2016) at Abasyn University Peshawar, Pakistan.

1st International Conference on “Prevention of Dengue and Infectious Diseases” as a speaker (28,1,2 February- March 2018) held at University of Swabi, Pakistan.

One day “Symposium on Infections In Immunocompromised Patients” held on 21st October, 2017 Editorial Role Worked as a review editor in the following journals

Microbiological Research

Journal of Pediatric Infectious Diseases

Annals of Microbiology

Language Proficiency

I can write, speak and understand Pashto, Urdu and English very well Chines and Arabic can speak also. Other Volunteer Designations

As a Microbiologist in Surge Laboratories Pvt. Ltd. Pakistan from 2012 to 2014.

Research Associate in Microbiology Laboratory Khyber Medical University from 2013 to 2018 during study.

Working as Volunteer Special Coordinator to Drugs for the anti-corruption and anti-narcotics association of Pakistan.

Member District -blood donor Society

President of Regional organization for development of our village council. Extra-Co-Curricular Activity

Volleyball still (University Team Captain,2008-12)

Basket Ball (University Team Player,2008-12)

Badminton (University Team player,2009-11)

Cricket (University Team player,2008-2012)

REFERENCES

Dr. Gixue Wang (PhD Supervioser)

Professor and Dean of

Key Laboratory for Biorheological Science and

Technology of Ministry of Education, State and

Local Joint Engineering Lab for Vascular Implants

College of Bioengineering Chongqing University,

Chongqing 400030

Email; ******@***.***.**

Dr. Hazir Rahman

Associate Professor Microbiology

Abdul Wali Khan University Garden Campus

Mardan Khyber Pakhtunkhwa, Pakistan

E-mail: ***********@*****.***.**

Phone No: +92-347-*******

Dr. Mohsin Shah

Assistant professor Physiology

Institute of Basic Medical Sciences

Khyber Medical University

Peshawae Khyber Pakhtunkhwa, Pakistan

E-mail: ******.***@*****.***

Mobile No. +92-347-*******

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