Alexandre B. Dimtchev

**** ***** ****, ***.*** Bethesda, MD 20816 202-***-**** ad00wl@r.postjobfree.com

Professional Profile

I am a highly skilled molecular biologist with extensive laboratory research experience and expert proficiency

in all standard molecular biology methods and in a variety of advanced techniques. These include but are not

limited to: Q-PCR, microRNA profiling with Taqman arrays, gene expression microarrays (Affimetrix), gene

cloning, Agilent 2100 Bioanalyzer and training with Next Generation Sequencing on Illumina platform – library preparation and clustering. My analytical thinking and logical reasoning are outstanding, and they allow me to

quickly grasp the essence of new problems and find solutions. I have experience with grant writing and hold

excellent foreign languages skills (Russian, German and Bulgarian).

Professional Experience

Research Associate Contractor

Meso Scale Diagnostics LLC, Rockville, MD 2022 to 2023

Electrochemiluminescence (MSD technology) Assay.

Combinatorial transfections of antibody producing cell lines.

Construction of recombinant plasmids for antibody expression.

Small scale preparation of plasmid DNA.

Senior Lab Technician Supervisor

Henry Jackson Foundation, USUHS, Department of Surgery, Bethesda, MD 2018 to 2022

Isolation of RNA from cells, tissues and FFPE samples.

Reverse transcription and Q-PCR with Taqman and SYBRGreen chemistry.

Transformation with bacterial plasmids and preparation of large preps from them.

Occasional construction of recombinant plasmids.

ELISA on supernatants of in vitro cultured human cells.

Biological Science Technician

USDA, ARS, BARC, Animal Genomics and Improvement Lab, Beltsville, MD 2016 to 2017

Isolated genomic, plasmid and BAC DNA.

Bisulfate treatment of genomic DNA.

Performed end-point PCR and cloning of resulting products.

Q-PCR for confirmation of Copy Number variation studies.

Prepared libraries for Next Generation Sequencing on Illumina platform.

Faculty Assistant

University of Maryland, Department of Animal and Avian Sciences, College Park, MD 2015 to 2016

Coordinated and performed the day to day activities in molecular biology and genetics.

Isolated genomic DNA from bull semen.

Performed end-point PCR, TA-cloning of the resulting products and plasmid DNA

isolation for Sanger sequencing.

Biological Science Laboratory Technician

USDA, ARS, BARC, Animal Genomics and Improvement Lab, Beltsville, MD 2011 to 2015

Prepared libraries for Next Generation Sequencing on Illumina platform.

Isolated genomic, plasmid and BAC DNA.

Performed end-point PCR and cloning of resulting products.

Conducted bisulfite treatment of genomic DNA and COBRA Assay.

Q-PCR and droplet-digital PCR for confirmation of Copy Number Variation studies.

Biomedical Research Contractor

University of District of Columbia, Department of Biological Sciences, Washington, DC 2010 to 2011

Analyzed data from metabolomics profiling of human cancer cell lines.

Isolated miRNA from body fluids of healthy humans and prostate cancer patients.

Performed Real-Time PCR analysis and TaqMan profiling of the isolated miRNA.

Research Associate

Georgetown University, Lombardi Cancer Center, Radiation Medicine, Washington, DC 1996 to 2010

Performed advanced molecular biology research in cancer and radiation biology.

Constructed and screened genomic and cDNA libraries to clone and amplify genes and

regulatory DNA sequences for the research projects in the lab.

Became “chief genetic engineer” of the group responsible for all recombinant DNA

assembly, expression and isolation of proteins and purification of plasmids for cells

transfection.

Contributed to project development and writing grants and scientific papers.

Education

PhD Bulgarian Higher Testimonial Committee, Bulgarian Medical Academy, Sofia, Bulgaria

Thesis: “Selection-genetic, physiological and biochemical investigations on the

gentamicin-producing Micromonospora purpurea var. violaceus BTCC 60”

MD Bulgarian Medical Academy, Sofia, Bulgaria

Awards

Department of Defense: Exploratory grant in ovarian cancer, 2008

USDA, Cattle Genome Research Projects: Certificate of Appreciation, July 2015

Peer-Reviewed Publications

1.N.M. Gervasi, A. Dimtchev, D.M. Clark, M. Dingle, A.V. Pisarchik, L.J. Nesti. C-terminal domain small phosphatase 1 (CTDSP1) regulates growth factor expression and axonal regeneration in peripheral nerve tissue. Scientific Reports, 11:14462, 2021

2.M. Dingle, S.D. Fernicola, J.F. de Vasconcellos, S. Zicari, C. Daniels, J.C. Dunn, A. Dimtchev, L.J. Nesti. Characterization of traumatized muscle-derived multipotent progenitor cells from low-energy trauma. Stem Cell Research & Therapy, 12:6, 2021

3.J.F. de Vasconcellos, W.M.Jackson, A. Dimtchev, L.J. Nesti. A microRNA signature for impaired wound-healing and ectopic bone formation in humans. The Journal of Bone and Joint Surgery. American Volume, 102(21):1891-1899, 2020

4.A. Srivastava, H. Goldberger, A. Dimtchev et al. Circulatory miR-628-5p is down regulated in prostate cancer patients. Tumor Biology, 35:4867-4873, 2014

5.A. Srivastava, H. Goldberger, A. Dimtchev et al. MicroRNA profiling in prostate cancer –the diagnostic

potential of urinary miR-205 and miR-214. PLoS One 8(10): e76994, 2013

6.A. Cheema, O. Timofeeva, R. Varghese, A. Dimtchev, K. Sheikh, V. Shulaev, S. Suy, S.

Collins, H. Ressom, M. Jung, A. Dritschilo. Integrated analysis of ATM mediated gene and protein expression impacting cellular metabolism. Journal of Proteome Research, 10:2651-2657, 2011

7.M. Jung, A. Dimtchev, A. Velena, A. Dritschilo. Combining radiation therapy with interstitial radiation

inducible TNF- expression for locoregional cancer treatment. Cancer Gene Therapy, 18:189-195, 2011

8. Y. Zhang, T. Carr, A. Dimtchev, N. Zaer, A. Dritschilo, M. Jung. Attenuated DNA damage repair by

trichostatin A through BRCA1 suppression. Radiation Research, 168:115-124, 2007

9. S. Chasovskikh, A. Dimtchev, M. Smulson, A. Dritschilo. DNA transitions induced by binding of

PARP-1 to cruciform structures in supercoiled plasmids. Cytometry, 68:21-27, 2005

10.M. Jung, Y. Zhang, A. Dimtchev, M.R. Subramanian, K. Suthanthiran, A. Dritschilo. Interstitial gene delivery in human xenograft prostate tumors using titanium metal seeds. Molecular Cancer Therapy,

6:655-659, 2004

11. T.Y. Kim, H.S. Jong, S.H. Song, A. Dimtchev, S.J. Jeong, J.W. Lee, T.Y. Kim, N.K. Kim, M. Jung,

Y.J. Bang. Transcriptional silencing of the DLC-1 tumor suppressor gene by epigenetic mechanism

in gastric cancer cells. Oncogene, 22:3943-3951, 2003

12. I. Trofimova, A. Dimtchev, M. Jung, D. Rosenthal, M. Smulson, A. Dritschilo, V. Soldatenkov. Gene

therapy for prostate cancer by targeting poly (ADP- ribose) polymerase. Cancer Research, 62:6879-

83, 2002

13. Y. Zhang, A. Dimtchev, A. Dritschilo, M. Jung. Ionizing radiation-induced apoptosis in ataxia-

telangiectasia fibroblasts. Roles of caspase-9 and cellular inhibitor of apoptosis protein-1. Journal

of Biological Chemistry, 276:288**-*****, 2001

14. G.D. Kim, Y.H. Choi, A. Dimtchev, S.J. Jeong, A. Dritschilo, M. Jung. Sensing of ionizing

radiation-induced DNA damage by ATM through interaction with histone deacetylase. Journal of

Biological Chemistry, 274:311**-*****, 1999

15. S.J. Lee, A. Dimtchev, M.F. Lavin, A. Dritschilo, M. Jung. A novel ionizing radiation-induced

signaling pathway that activates the transcription factor NF kappaB. Oncogene, 17:1821-1826, 1998

16. M.E. Smulson, D. Pang, M. Jung, A. Dimtchev, S. Chasovskikh, A. Spoonde, C. Simbulan-Rosenthal, D.

Rosenthal, A. Yakovlev, A. Dritschilo. Irreversible binding of poly(ADP)ribose polymerase cleavage

product to DNA ends revealed by atomic force microscopy: possible role in apoptosis. Cancer Research,

58:3495-3498, 1998

17. M. Jung, Y. Zhang, A. Dimtchev, A. Dritschilo. Impaired regulation of nuclear factor-kappaB results in

apoptosis induced by gamma radiation. Radiation Research, 149:596-601, 1998

18. M. Jung, S.A. Lee, A. Kondratyev, A. Dimtchev, A. Dritschilo. Phosphorylation of IkB by the ATM

gene product. Cancer Research, 57:24-27, 1997

19. M. Jung, A. Dimtchev, Y. Zhang, A. Dritschilo. Identification of genes involved in carcinogenic progression of

ataxia-telangiectasia cells. Radiation Oncology Investigations, 3:272-278, 1996

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