Project Higher Secondary

CURRICULUM VITAE

SURESH KUMAR. K

E-mail: *********************@*****.***

Contact number: 979*******

OBJECTIVE

To learn and function effectively in an organization and be able to deliver to the bottom-line. To constantly upgrade my knowledge and skills and make a difference in whatever I do.

TECHNICAL PROFILE

Course

Institution

Board/ University

Year of Passing

Percentage

M. Sc Biochemistry

PSG College of Arts & Science

Bharathiar University

April 2011

80

B. Sc Biochemistry

PSG College of Arts & Science

Bharathiar University

April 2009

83

Higher secondary

(XII)

Suburban

Matriculation higher secondary school

Matriculation

April 2006

85

SSLC (X)

Suburban

Matriculation higher secondary school

Matriculation

April 2004

90

RESEARCH EXPERIENCE

•Worked as a Research Fellow in Karunya University for a period of one year and six months in DBT-RGYI project. The project was on the implementation of nanoparticles and microbes in textile dye effluent treatment.

Techniques learnt – HPLC, GC-MS, Chromatography, Anaerobic work chamber, FTIR, SEM, XRD

•Worked as a Research Fellow in Christian Medical College. The objective of the project is to evaluate the possible mediators of the mild phenotype observed in severe hemophilia A. Also worked on the characterization of FVII mutant invitro and on the mutation screening of Gray Platelet syndrome patients.

Techniques learnt – Molecular diagnosis of bleeding disorders, Gene therapy, Cell culture techniques, Stem cell technology, Animal handling.

PROJECT WORK

1.Under Graduate Project: “A study on the anti-oxidant and antimicrobial activity of Prunus cerasoides” at PSG College of Arts & Science.

Techniques learnt – Spectrophotometry, Colorimetry, HPLC, Mass spectrometry, GC-MS

Project overview – The anti-oxidant activity of the bark extracts of Prunus cerasoides was estimated by its ability to scavenge hydroxyl radicals, nitroxide radicals, and sulphoxide radicals. There was a significant increase in the scavenging activity of the plant extract compared to the standard used. Similarly, the extract also showed potent anti-microbial activities against Escherichia coli, Proteus vulgaris, Salmonella typhi, and streptococcus.

2.Post Graduate Project: “Comparison of Agrobacterium and particle gun mediated gene transformation methods in Eucalyptus camaldulensis” at Institute of Forest Genetics and Tree Breeding (IFGTB-ICFRE).

Techniques learnt – Particle bombardment, Gene gun method, Cell culture, Transformation,

Transfection

Project overview - The aim of the project was to compare the efficiency of transformation achieved by different methods such as Agrobacterium tumefaciens mediated transformation and particle gun mediated transformation of Eucalyptus camaldulensis. Additionally, a combination of both Agrobacterium and particle gun mediated transformation was also done to check its efficiency. Selectable markers namely GUS and GFP were used in the screening of the transformants. The number of transformants was higher in the Agrobacterium mediated transformation.

INTERNSHIPS

Under Graduate Internship: At Mrithasanjivini Labs, R.S.Puram, Coimbatore from May 2008-June, 2008.

Hands - on training:

•Biochemical analyses.

•ELISA and EIA

Post Graduate Internship: Two weeks training on “Molecular medicine” at Regional Cancer Centre (RCC), Trivandrum

Immuno-histochemistry

RT-PCR

TECHNICAL EXPOSURE

BIOCHEMICAL TECHNIQUES

Analysis of biomolecules

BIOTECHNOLOGICAL TECHNIQUES

Biolistics & particle bombardment

Gel Electrophoresis

Bacterial Transfection and transformation

CELL CULTURE TECHNIQUES

Culturing and passaging cell lines, MTT, recombinant studies using cell lines

Cell transfection and transformation studies

Invitro toxicity and cell viability studies

Site directed mutagenesis

MOLECULAR BIOLOGY TECHNIQUES

DNA and RNA isolation

Polymerase chain reaction

Polyacrylamide gel electrophoresis (PAGE)

Conformational sensitive gel electrophoresis (CSGE)

Western blotting

Gene cloning

STRENGTH

Open minded to learn new ideas in the chosen field, meticulous to details, perfection to the last detail, hardworking, adaptable to any situation, team player coupled with proactive attitude, efficient and timely execution of projects.

REFERENCES

1.Jayandharan G Rao PhD

Associate Professor

Department of Haematology

Scientist (Adjunct)

Centre for Stem Cell Research

Christian Medical College, Vellore-632004, TamilNadu India.

Email: ***@**********.**.**

2.Dr. D. Brindha

Associate Professor and Head of the Department,

Department of Biochemistry,

PSG college of Arts and Science,

Coimbatore-14

Email: ****************@*****.***

DECLARATION

I do hereby declare that the particulars of information and facts stated herein above are true, correct and complete to the best of my knowledge and belief.

SURESH KUMAR.K

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