Wen Xi

** *** ******* **. *** ** Seabrook, NH 760-***-**** aczb9n@r.postjobfree.com

Education

Ph.D. Biochemistry, University of New Hampshire, Durham NH Dec. 2015

M.S. Molecular Biology, Peking Union Medical College, Beijing China Jun. 2010

B.S. Biology, Wuhan University, Wuhan China Jun. 2006

Research Experience

Post-Doc. Research Associate, University of NH, Durham, NH Oct 2016 – present

Structure-based discovery of nematicides targeting phosphodiesterase enzymes in phytoparasitic nematodes

Establishing expression system of human, C. elegans and M. hapla phosphodiesterases (PDE1, PDE3, PDE4 and PDE10), purifying functional PDE proteins for drug binding assays.

Post-Doc. Research Associate, University of NH, Durham, NH Jan 2016 – Oct 2016

Study of polyglutamine toxicity with Huntington’s disease yeast model

Analyzed the property of the discrete polyglutamine aggregates previously found in the model system with a variety of yeast strains.

Prepare and collect data required to the response of the publication currently under review (Wide Distribution Analysis of the samples, frictional ratio calculation and western blotting)

Graduate Research Assistant, University of NH, Durham, NH 2011 – 2015

Study of polyglutamine toxicity with Huntington’s disease yeast model

Discovered a set of soluble discrete polyglutamine aggregates correlated with the toxicity of the prion forming polyglutamine in the model system with Analytical Ultracentrifugation.

Characterize the role in translation of Ribosomal complexes contains SBP1

Discovered the mRNA degradation promoter SBP1 binding to the actively translating mRNP at late elongation/early termination phase. SBP1 is mutually exclusive to most of the translation initiation factors in this complex.

Determined the stoichiometry of several translation factors (eIF4E, eIF4G1, PAB1, etc.) in the actively translating mRNP

Graduate Research Assistant, Peking Union Medical College, Beijing China 2006 – 2010

Constructed HEK293 cell-lines expressing HCV receptors CD81-eGFP and CLDN1-RFP on membrane

Determined the acute toxicity of Single-Wall carbon nanotube to the lung is through cytokine storm triggered by NF- B pathway.

Publications

1.Xi W, Wang X, Laue TM, and Denis CL (2016) Multiple discrete soluble aggregates influence polyglutamine toxicity in a Huntington's disease model system. Sci Rep. 2016 Oct 10;6:34916.

2.Wang X, Xi W, Toomey S, Chiang YC, Hasek J, Laue TM, Denis CL. (2016) Stoichiometry and Change of the mRNA Closed-Loop Factors as Translating Ribosomes Transit from Initiation to Elongation. PLoS One. 2016 Mar 8;11(3).

3.Zhang C, Wang W, Park S, Chiang Y-C, Xi W, Laue TM, and Denis CL (2014) Only a subset of the PAB1-mRNP proteome is present in mRNA translation complexes. Protein Sci. 2014 Aug;23(8):1036-49

4.Wang X, Zhang C, Chiang Y-C, Toomey S, Power MP, Granoff M E, Richardson R, Xi W, Lee D J, Chase S, Laue T M and Denis C L, (2012) Use of the novel technique of analytical ultracentrifugation with fluorescence detection system identifies a 77S monosomal translation complex. Protein Sci. Sept.; 21(9): 1253–1268

5.Zhang Y, Deng J, Zhang Y, Guo F, Li C, Zou Z, Xi W, Tang J, Sun Y, Yang P, Han Z, Li D, Jiang C (2013) Functionalized single-walled carbon nanotubes cause reversible acute lung injury and induce fibrosis in mice. J Mol Med (Berl). Jan; 91(1):117-28

Professional Skills

Molecular Cloning and Cellular Biology

Plasmid design and construction, PCR, primer design, DNA extraction, plasmid transformation

Yeast genetics: Plasmid transformation and selection, gene knock-in/out, mating and sporulation

Mammalian cell line culture, transfection and construction

Protein Biochemistry

Protein expression in E. coli., yeast and mammalian cell

Protein purification: ion-exchange chromatography, molecular filtration, affinity chromatography, ultracentrifugation (sucrose gradient for protein/CsCl gradient for DNA)

Protein analysis: analytical ultracentrifugation, SDS-PAGE, western blot, enzymatic assays, ELISA

General Laboratory skills

Operation of ultracentrifuge, fluorescent microscope, protein purification system, UV spectrometer, western image system and other major equipments.

Animal experiments: Polyclonal antibody preparation, Mice experiments, dissection, specimen collection, H/E staining

Experience in using Microsoft Office, Sedfit, Ultrascan3, Sedanal, Adobe Photoshop and other computer software.

Awards and activities

May 2014 UNH Summer TA Fellowship

Dec. 2013 UNH MCBS seminar presentation:

Studying de novo translational complexes co-purified with Sbp1 in Saccharomyces cerevisiae

Nov. 2011 UNH MCBS seminar presentation:

Identifying mRNP Complexes Involved in Translational Regulation in Saccharomyces cerevisiae with Analytical Ultracentrifugation

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