Ambili Menon, PhD

(Authorized to work in the US for any employer)

a(Authorized to work in the US for any

employer)

(Authorized to work in the US for any

employer)

**** ******** ******, *********e-60563 Illinois

630-***-****

acz9bn@r.postjobfree.com

PROFESSIONAL SUMMARY

Innovative, accomplished and highly motivated Research Biologist with extensive background in Immunology, Molecular biology, Cell biology and Biochemistry.

Experience working in diverse area of research like Inflammation, Allergy, Oncology and Signal transduction

Proven ability to initiate interdisciplinary collaboration as well as leadership and supervisory abilities developed through mentoring graduate students, leading institution-wide events and leading journal clubs.

Skilled at managing, analyzing and presenting data to a wide variety of audiences. Authored/delivered scientific presentations / journal publications.

High analytical skills with a passion for learning new technologies.

• Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nunc arcu mi, pretium quis dolor id, iaculis euismod ligula. Donec tincidunt gravida lacus eget lacinia.

• Cras sed sapien quam. Sed dapibus est id enim facilisis, at posuere turpis adipiscing. Quisque sit amet dui dui. Donec tincidunt gravida lacus eget lacinia.

• Donec aliquam augue nec gravida lobortis. Nunc arcu mi, pretium quis dolor id, iaculis euismod ligula. Donec tincidunt gravida lacus eget lacinia.

WORK EXPERIENCE

Conducted independent research for DFG (SPP1394) funded project to elucidate the role of PEP Phosphatase in signaling pathways critical for Mast cell allergic inflammation

Discovered that PEP interacts with early signaling components of mast cells through substrate trapping assays and positively regulates anaphylaxis in mice.

Developed PEP inhibitors in collaboration with labs of University of Utah & Indiana University.

Designed and performed in vitro-in vivo assays for screening and determining the potency and efficacy of the phosphatase inhibitors in rescuing mice from Anaphylactic shock, which led to publication in Journal of Medicinal Chemistry and Allergy. The study proved to be a starting point for the development of clinically useful protein tyrosine phosphatase inhibitors.

Utilized strong multi-tasking ability to prioritize and balance numerous simultaneous experiments. Designed hypothesis and conducted experiment for achievement of research goals; oversee multiple projects and monitored progress, set priorities and meet manuscript and project deadlines.

Coordinated all aspects of laboratory: inventory, maintenance of equipment, workflow planning, safety, and weekly research meetings. Developed and designed lab SOPs, Lab E Notebook. PhD Research Fellow Karlsruhe Institute of technology, Germany 2010-2013

Research focused on ceramide-1-phosphate (C1P)/cPLA2α interaction as target for anti-inflammatory mediators.

Constructed a Fluorescent tagged cPLA2α recombinant adenovirus and identified inflammatory agonist that stimulates translocation of cPLA2α to membranes in carcinoma cells through Subcellular fractionation and Immunoblotting and confocal microscopy

Identified C-1-P binding site to be crucial for the translocation through Site directed mutagenesis. The mutation decreased the cPLA2α translocation even in presence of agonist.

Developed a novel strategy employing Protein Synthesis Inhibitors (PSI) to reduce Neutrophil extravasations following Tissue-Injury which could form a basis for a therapy to prevent Acute Respiratory Distress Syndrome (ARDS)

Designed a trans-well insert seeded with Endothelial cells (HMEC-1) which mimicked the in-vivo system of endothelium of blood vessel and tissue milieu for the transmigration experiments.

Investigated the efficacy of PSI on the transmigration of Neutrophil in presence of inducers. Research Intern Virginia Commonwealth University, USA 2007-2008

Conducted independent research to improve Aspergillus niger Aj2k (Lab isolate) strain for increased Pectinase production. Developed methods to optimize fermentation medium using statistical designs which increased Pectolytic enzymes production with higher enzyme activity.

Characterized the enzymes on the basis of activity and molecular weight using staining techniques and SDS-PAGE renaturing gel, further pH, Tm and Km of these enzymes were determined..

Interpreted complex statistical data and compiled data in collaboration with senior scientists. Demonstrated ability to present complex data in a clear concise manner for a range of audience. MSc Research Fellow The Maharaja Sayajirao University, India 2004-2006 Molecular

Biology

Biochemical Histology Cell Culture Animal handling Microbiology Statistics & Software

DNA/RNA

extraction,

qPCR

DNA gel

analysis

Microarray

Luciferase

Reporter

Gene assay

Site-directed

Mutagenesis

Spectrophotometer

Confocal

Microscopy

SDS-PAGE

Protein

Purification

Flowcytometry

FACS

MTT assay

Immunohisto

Chemistry

Staining

ELISA

Luminex

Primary cells

Cancer cells

Isolate

PMNs HMC,

BMMC

Recovery

& cryogenic

freezing

Transfections

Blood sampling

Tail/vein/cardiac

puncture

Injection:

intravenous,

intraperitoneal,

sub-cutaneous

Euthanasia

Maintain mouse

Colonies

Anaphylaxis

Bacteria

culture,

Plating

Staining, MIC,

Plaque assay

Mycoplasm

Virology

testing,

ANOVA

Sigma Plot

Graphpad

Prism

Flowjo

Cell quest

Mev

ImageJ

MS office

Excel

Adobe

photoshop

Led the process of training and managing postgraduate students in laboratory techniques like cell culture, biochemical and molecular biological assays.

Lectured undergraduate class on Immunology and cell biology. Teaching Assistant Karlsruhe Institute of technology, Germany 2010-2012

Assisting students to prepare for GRE, GMAT, TOEFL, IELTS examination and University admission as well as Visa documentation and visa interviews.

Admission Assistant Enbee Education Pvt ltd,India 2006-2007 EDUCATION

Karlsruhe Institute of technology, Germany PhD Biological science & Chemistry 2010-2013 (GPA 4.0)

The Maharaja Sayajirao University, India MSc Microbiology 2004-2006 (GPA 4.0)

The Maharaja Sayajirao University, India BSc Zoology, Botany, Chemistry 2001-2004 (GPA 4.0) CERTIFICATION

CALISO Corporation, USA FDA GLP & cGMP In progress

Karlsruhe Institute of technology, Germany Laboratory Veterinary Practice 2010-Present TECHNICAL EXPERIENCE

AWARDS & ACTIVITIES

Doctorate Scholarship: The Bio-Interfaces International Graduate School Karlsruhe Institute of Technology 2010.

Oral and Poster presentation at EMBRN-COST International Mast cell & Basophil Meeting (2010-2012).

Awarded first prize (2002 and 2005) in debate competitions organized at The Maharaja Sayajirao University.

Best public speaker award by Baroda Metropolitan Junior Chamber

Meritorious position in the TSI Chemistry Olympiad.

Meritorious position in International Science Assessment-The University of New South Wales, Australia

Commendation certificate for best essay by The Royal Commonwealth Society, London

Volunteered to donate blood in blood donation drives conducted by Shri Sayaji General Hospital for 4 yrs.

Coordinated & organized food drives in schools, churches and other community organizations.

German language proficiency-intermediate.

Trained Indian classical dancer (Bharatnatyam, Kuchipudi, Mohiniattam)

Contact this candidate