Professional Experience Research

CARLA A. SMITH

** ****** *****, ********, ** ****0; (Cell) 917-***-****; E-mail **********@***.***

Research Associate

Research-oriented Scientist with over 11 years of experience in management of high-throughput transgenic mouse colonies and comprehensive research in Microbiology, Immunology, Biochemistry, Molecular Biology.

Education and Certificates

Masters of Science, Biotechnology – John Hopkins University, expected graduation date 2018

Bachelor of Science, Chemistry – Hunter College, 2005

Certificate of fitness for supervising non production chemical laboratories (C14)

Certificate in Human Subjects Research Education

Certificate of Achievement in completion of BD FACSCelesta Operator Course

Professional Experience

Rutgers University

Research Associate 2016 – Present

Studying cognitive impairment within the racial disparity of individuals that are HIV infected and have NeuroAIDS

Expansion and quantification of stock HIV ADA virus using CEM cells

Expansion of recombinant plasmid of HIV, viral transfection and viral concentration using calcium phosphate

Performance of HIV entry assay utilizing beta lactamase and analysis of FRET

Design and implementation of novel kinetic assays that identify neuropharmacology and pharmacodynamics tritiated ART compounds in primary human brain

Examination of differential protein and RNA expression among HIV ethnicities using primary human tissue

Expertise in immunophenotyping of HIV infected patient samples using Immunoblotting, Western Blot, qRT-PCR, and Biotin assays

Real time acquisition and analysis of protein channel kinetics during HIV infection at the single cell level using live cell fluorescent microscopy

Maintenance and training of new staff and students on the multicolor flow cytometry using the BD FacsCelesta

Designing and acquisition of multicolor flow cytometry panels to identify rare populations of human PBMS using up to 10 colors.

Develop and implement Standard of Operations Procedure for working with HIV

Memorial Sloan Kettering Cancer Center

Senior Research Technician 2015 – 2016

Optimizing Immuno-therapy experiments using Xenograft mice

Experience in working with sterile cell culture techniques for the growth and maintenance of cell lines

Isolation of mononuclear cells from human peripheral blood

Expansion of DNA by transforming in E coli and DNA purification

Transfect and transform DNA into Jurkat cells and human T cells using pLenti virius

Tetramer staining assays using multi-parameter flow cytometry and analysis

Extract DNA from cancer cell lines and PBMC’s for HLA-typing

Prepare cancer cell lines and PBMC’s for RNA then cDNA for PCR’s and real-time PCR’s

Implantation of tumors cells, T cells and cytokines into xenograph mice via intraperitoneal and subcutaneous

Monitor by bioluminescent imaging the tumor growth of xenograph mice

Dissection of xenograph mice tumors and organs

Analyze slides of the H and E staining of organs and tumors

Cell culture, including passaging, expanding, cryopreservation, thawing and processing cells

Macromolecular Therapeutic Development Facility, Albert Einstein College of Medicine Research Technician C 2013 – 2015

Building, maintaining and screening libraries of small molecules for activities against proteins that mediate cellular processes

Following the SOP, optimization of new protocols and assay development for cell- cell and protein binding assays

Cloning

Normalization of DNA in 96-well plates using the Biomek Fx liquid-handling robotics

Transformation in E coli using 96 wells plates

Mammalian cell culture (HEK cells)

Transient transfection of mammalian cells in 96 well plates; reading and analyzing of data via BD Accuri/Intellyct

Cell sorting and analysis of data via BD Aria

Small scale and large scale protein expression, endotoxin free protein purification from HEK cell lines

Albert Einstein College of Medicine 2005 - 2013

Research Technician B

Management of over 30 transgenic and knockout mouse colonies some having over 3 strains per colony to be genotyped via PCR and flow cytometry

Supervised and trained new post docs and students in the laboratory

Extracted mouse pancreas by profusion for islet isolation

Backcrossed transgenic strains to NOD mice

Developed humanized mouse models for future research

Designed a construct for the development of progeny transgenic mouse model

Breed and maintained transgenic mouse colonies and ensured accurate data records

Communicate with husbandry, veterinary, and lab partners to coordinate animal space for breeding and housing

General laboratory and equipment maintenance

Inventory and purchase of reagents and supplies

Hunter College 2003 - 2005

Minority Biomedical Research Support Trainee

Development and characterization of a photoactive probe to examine benzophene-binding on adenine-binding proteins

Extracted and collected organic samples using Column, Gas and High - Performance Liquid Chromatography (HPLC)

Coached freshman students entering the lab

Analyzed data using (spectroscopy) UV, IR, and NMR

Synthesized organic compounds by crystallization method

Columbia/ Hunter Summer Research Program 2004 – 2004

Summer Trainee

Investigated the activation of the TGF-beta pathway in atherosclerotic lesions of MMP – 9 transgenic mice in vivo

Extracted expressed and purified protein

Analyzed data by performing different techniques such as (Western Blot, Immunohistochemistry and Gelatin- zymography)

DNA and RNA preparation and isolation

Bacteria and cell tissue culture

St. Barnabas Hospital

Outpatient Clinic Supervisor 1995 – 2000

Managed a high volume off-site clinic for the hospital, this included over 15 different Specialists and over 40 rotating Medical Doctors

Supervised the staff of clinical registration operations and Medical Records

Communicated and assisted doctors with their cancelled and rescheduled patients appointments, then coordinated with my staff to address these issues

Prepared bi-weekly payroll

Prepared and conducted Annual Staff Performance Reviews

Maintained supportive relations with and insured productivity among the staff

Trained new employees on clinical operations

Trouble shoot the HBO computer system for the clinic

Organized adequate supplies for Registration, Medical Records and Housekeeping Departments

Addressed patient problems and promoted immediate resolutions

Publications

Antal, Z., Baker, J., C. Smith, I. Jarchum, J. Babad, G. Mukherjee, Y. Yang, J. Sidney, A. Sette, P. Santamaria, and T. P. DiLorenzo, 2012, Beyond HLA-A 0201: New HLA-Transgenic Nonobese Diabetic Mouse Models of type 1 Diabetes Identify the Insulin C-Peptide as a rich Source of CD8+ T Cell Epitopes .J Immunol 11:5766-75

Mukherjee, G., R. Chaparro, J. Schloss, C. Smith, C. Bando, and T. P. DiLorenzo. Glucagon-reactive Islet-infiltrating CD8 T Cells in NOD Mice. Immunology Accepted Article, doi:10.1111/imm.12415

Poster Session

“Synthesis of Photoactive Peptide Nucleic Acid” Annual Biomedical Research Conference for Minority Student, 2003, San Diego, California

“Synthesis of Molecular Baits for Protein Fishing” City University of New York Conference in Science and Engineering, 2004, New York

“Activation of the TGF-beta pathway in atherosclerotic lesions of MMP-9 transgenic mice” Annual Biomedical Research Conference for Minority Student, 2004, Dallas, Texas

Awards and Honors

Minority Biomedical Research Support-Research Initiative for Scientific Enhancement (MBRS-RISE) Scholar, Hunter College- CUNY - 2003

Avon Tuckman Award, Summer Research – 2004

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