Kumud Chandra Das

267-***-****; email:acz2l7@r.postjobfree.com

SUMMARY (WORK EXPERIENCES):

• Organization, management, Laboratory safety and use of PPE.

• Hands-on training in tools and techniques of modern biology.

• Purchasing, Record keeping, inventory, chemical hygiene.

• Experiences in Good Laboratory Practices (GLP) as set forth by 21 CFR.

• Preparation and Approval of Standard Operating Procedure (SOP).

• Familiar with GCP, ICH, cGMP, ISO.

• Familiar with state and Federal regulations-OSHA, USDA, EPA., HIPPA.

• Data analysis, presentation and discussion. Computation and literature search.

• Knowledge of quality control and quality assurance.

ACHIEVEMENTS:

• Participated in a number of research projects. Attended meetings and conferences.

• Aseptic techniques and Cell/tissue culture : Routinely maintained primary cell cultures and cell lines

• Cultivated and propagated bacteria.

• Grown various strains of bacteria on liquid and solid media. Routinely used bacterial stains, Prepared and performed bacterial staining’s (including Gram stain, Acid-Fast stain, and Giemsa stain).

• Processed blood, isolated lymphocytes, immortalized with EBV virus. Maintained primary culture, isolated nucleic acids, proteins, did assays.

• Did sterility testing of culture media, serum, buffers and other solutions. Sterilized the biosafety hood.

• Worked with Influenza viruses. Worked with Sendai viruses and SIV. Cultivated and propagated influenza viruses, did quantitative and qualitative assays of viral particles. Involved in the preparation of subunit vaccine. Did protection study to determine the immunity to mucosal infection.

Worked with clinical blood (PBM) collected from HIV sero-positive and sero-negative individuals.

• Prepared various solutions/buffers, culture media.

• Cellular Assays (ELISA, MTT), Hem agglutination, Hem agglutination-inhibition assays. Viral Plaque assays.

• Protein & Nucleic acid extraction and assays

• Microscopy, histological, Immunohistological, Immunofluorescent & Immuno Cytochemical methods including H&E and Wright’s blood stains.

• Animal handling and Husbandry. Maintained transgenic mice colony.

• Purchase Laboratory materials. Inventory, storage and disposal. Worked with hazardous materials including chemicals, biological wastes, radioactive waste, and bacteriological wastes.

PROFESSIONAL EXPERIENCE:

HISTOTECHNICIAN. McLendon Clinical Laboratory.-2016(Dec.) UNCH, UNC, Chapel Hill, NC.

Achievements: Processed biopsies for histopathological analysis.

SCIENCE TECHNICIAN. 2011-Nov.2015.City of Philadelphia, PWD, Philadelphia, PA

Achievements: Data gathering and retrieval from various sources, data input and preparation of report, report of data to agencies like EPA, as well as inside the departmental communities. Analysis of laboratory data, record keeping. Communication. LIMS, COC.

RESEARCH ASSISTANT.2008-2009: Thomas Jefferson Univ. Philadelphia, PA.

Achievements: Managed Clinical Molecular Pathology Laboratory (CLIA certified), Neuromuscular Division, Dept. of Neurology. Processed biopsies for analysis and diagnosis. Prepared various solutions, troubleshoot equipment, did quality assurance/quality control, chemical hygiene. Dispose biohazards following SOP. Record keeping and inventory.

BIOLOGY TECHNICIAN/LAB.MANAGER. 2006-2008: Saint Peter’s University NJ.

Achievements: Responsible for the overall laboratory preparations for the department of Biology. Aseptic techniques/ sterilization. Worked with bacterial culture, propagate various strains in solid and liquid media for experiments. Mammalian cell culture for molecular biological analysis. Troubleshoot laboratory equipment’s .Chemical hygiene. Storage and disposal of biohazards. Supervise student assistants.

SR.LABORATORY TECHNICIAN/LAB.MANAGER. 1994 –2005: RUTGERS’ NJ. Achievements: Participated in a number of research projects. Organized and managed laboratory.

Worked with in vitro and in vivo systems.

• Maintained mammalian cell lines namely, PR1and primary cell cultures (pituitary lactotrophs, folliculostelate cells, and lymphocytes). Treated cells with drugs/chemicals.

• Isolated lymphocytes from human PBM and immortalized using EBV Did proliferation assays. Isolated folliculstellate cells, prolactin cell (lactotrophs) from mouse and rat pituitary glands and cultured.

• Isolated and culture spleenocytes.

• Isolated proteins, nucleic acids and analyzed. Worked in the Cell & DNA Repository (RUCDR).

• Worked with biohazard substances-chemicals, radioisotopes, infectious agents. Completed training in laboratory safety and chemical hygiene offered by Rutgers’ Environmental Health and Safety Department (REHS).

• Attended and participated in scientific meetings and conferences.

UNIVERSITY OF MED. & DENTISTRY (UMD NJ).1985-1987. Research/Teaching Specialist.

Achievements: Research and management of laboratory. Worked with cell/tissue culture, bacteria and viruses, laboratory animals.

• Isolated mouse embryonic brain (MEB) cells, astrocytes for primary culture. Did primary culture of embryonic brain cells, astrocytes, and renal cells. Micro dissected kidney tubules for renal cell culture from rabbit and human polycystic kidney. Treated cells with various agents (viral particles, chemicals, growth factors).

• Propagated influenza and Sendai viruses in chicken embryo. Isolated purified and quantified viral particles. Participated in the preparation of subunit vaccine.

• Participated in scientific meetings and seminars.

EDUCATION AND HONORS:

PhD (ABD) In CELL & DEV.BIOL., RUTGERS’, NJ

M.A. In Biology. Hofstra University, Hempstead, NY. (Graduate fellowship)

M.Sc. In Zoology. Gauhati University (GU). India. State Merit Scholarship.

B.Sc. Honors (Majors: Chemistry, Botany, and Zoology). Cotton College (GU). India

PhD Fellowship of UGC (NEW DELHI, India). Gauhati University. India. 1977.

PUBLICATION:

• Expression of D2 dopamine receptors, D2L and D2S modulated by alcohol”. Sochi Omizu, K.Das, D.Sarkar. Alcoholism: Clinical and Experimental Research. Vol.26. No.6. May 2002;(Abstract)

• Determination of prolactin mRNA levels in pituitary gland after alcohol treatment using Real-Time PCR techniques”. Souchi Omizu, Sudhir Jain, Kumud Das, Dipak K. Sarkar. Alcoholism: Clinical and Experimental Research. Vol 25.No.5.2001 (Abstract)

• “Lipid matrix-based subunit vaccines: a structure-function approach to oral and parenteral immunization.” Gould-Fogerite S., Edghill-Smith Y., Kheiri M., Wang Z., Das,K., feketeova E., Canki, M., Mannino RJ. Aid Research & Human Retroviruses. 10 Suppl.2: S99-103. 1994. (Paper)

• “Abdormal growth factor response by human adult polycystic kidney disease (APKD) epithelia in vitro”. K.Das, P.A.Gabow, P.D. Wilson. Kidney international.35(1):31 Jan.1989 (Abstract)

• “Preliminary observations on cortical granule composition in eggs of Brachydanio .” K.Das, N.Hart, J.Woliniski. Biological Bulletin. 170(3) 1986 (Abstract)

• American Zoologist. 25(4): 97(A). 1985 (Abstract).

Contact this candidate