State University High School
Resume:
CURRICULUM VITAE
Michael Abdelsayed, Ph.D.
********@***.***
Simi Valley CA, 93065
EDUCATION
****-**** **.*., ********** of California, Irvine
Department of Molecular Biology and Biochemistry Graduate Program
Advisor: Andrej Lupták
2008 B.S. in Biochemistry, California State University, Channel Islands
RESEARCH EXPERIENCE
January 2011 – November 2016
University of California, Irvine
Advisor: Andrej Lupták, Ph.D. Department of Pharmaceutical Sciences, Department of Chemistry, Department of Molecular Biology and Biochemistry
Dissertation titled “Discovery of functional RNAs through laboratory evolution”
Duties:
● Lab manager while P.I. was on sabbatical.
● Rotating laboratory duties including: ordering supplies, preparing reagents, diradioactivity safety, designing oligos, and maintenance of lab equipment.
● LEAPS - Laboratory Experiments and Activities in the Physical Sciences. Developer a and coordinator of outreach activities with local 6th-8th grade students.
Accomplishments, Related Skills:
In vitro selections for genomic aptamers:
● Amplification, transcription, and purification of genomic libraries.
● Column binding experiments with multiple targets on beads.
● Cloned and transformed DNA into cells and sequenced individual clones.
● Selective 2’-hydroxyl acylation analyzed by primer extension (SHAPE) of selected l libraries and individual sequences.
● Deep sequencing of libraries.
In vitro selection for novel riboswitches:
● Design of multiple selection methods.
● Fluorescence toe-hold assay was adapted and performed for feedback of s riboswitch activity.
● Collected kinetic data of libraries and individual sequences using a fluorimeter and m microplate reader.
● Design of fluorescence reporter plasmids based on riboswitch expression.
● Kinetic expression studies of individual sequences through in vitro transcription and P PAGE analysis.
● Structure probing of individual sequences by SHAPE and in-line probing followed by d denaturing PAGE gel analysis.
September – December 2010
University of California, Irvine
Advisor: Klemens Hertel, Ph.D. UCI School of Medicine Department of Microbiology & Molecular Genetics
● In vitro splicing of RNA.
● Cell culture, RNA extraction from cells.
● Tested mRNA transcripts for nonsense-mediated decay (NMD).
August 2009 – May 2010
California State University, Channel Islands
Advisor: Blake Gillespie, Ph.D. Department of Chemistry
TEACHING EXPERIENCE
Teaching Assistant (2010-2016) University of California, Irvine
Department of Biological Sciences
Molecular Biology M116L lab (1 quarter)
Biochemistry 98 discussion (2 quarters)
Microbiology 188L lab (1 quarter)
Department of Pharmaceutical Sciences
Topics in Pharm SCI (1 quarter)
Biopharmaceutics & Nanomedicine 174L lab (1 quarter)
Molecular Pharmacology 2 discussion (1 quarter)
Medicinal Chemistry 177L lab (I quarter)
Resident Scientist Santa Ana High School
Biology AP and CP (2012-2013) On a weekly basis.
Biology AP and CP (2013-2016) On a volunteer basis.
AWARDS AND HONORS
2012-2013 NSF GK-12 Fellowship (NSF GrantDGE-0638751)
2016 Ayala School of Biological Sciences Teaching Award
PRESENTATIONS
Advancement to Ph.D. candidacy examination
2013 In vitro selections for a lanthanide aptamer. UC Irvine, Department of Molecular Biology & Biochemistry
Committee members: Andrej Lupták, Paul Gershon, Thomas Poulos, Michael Mulligan, Klemens Hertel
Posters
2013 In vitro selections for a lanthanide aptamer.
Irvine, Department of Molecular Biology & Biochemistry, department retreat, Lake Arrowhead, CA
2013 In vitro selections for a lanthanide aptamer. AAAS Student Poster Competition, Boston, MA.
PUBLICATIONS
Abdelsayed M, Ho B, Vu M, Spitale RC, Lupták A. High-throughput aptamer discovery through Apta-Seq and its application to genomic SELEX of human ATP aptamers (submitted to ACS Chemical Biology).
REFERENCES
Andrej Lupták, Ph.D. Department of Pharmaceutical Sciences, Department of Chemistry, Department of Molecular Biology and Biochemistry, University of California, Irvine
Phone: 949-***-****
Email: *******@***.***
Klemens J. Hertel, Ph.D. Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine
Phone: 949-***-****
Email: *******@***.***
Nelida Rojas, Santa Ana High School Biology Teacher
Phone: 714-***-****
Email: ******.*****@*****.**
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