Research Specialist

TARANA A. KUDROLLI

**** ******* *****

Frederick, MD 21704

Phone#: 301-***-****/ 240-***-****

*********@*******.***

SUMMARY

My name is Tarana Kudrolli. I am applying for the position advertised.

While employed as a research professional at the Brady Urological Labs of Johns Hopkins University, I have been a part of a number of significant Prostate cancer (PCa) research projects as elaborated in my resume. I am knowledgeable with the scientific tools, equipments and research methods in Molecular biology, and apply the strictest of care, meticulous attention and safety protocols to every assignment.

I have two Masters degrees, one in Biochemistry from University of Bombay, the second in Biotechnology from Johns Hopkins University, and over a period of time I have worked as a Research Technologist and then as a Research Specialist.

I have focused on research in Prostate cancer (PCa) with pFEX Adenoviral Vector System, to design Conditionally Replicating Adenoviruses (CRADs) which replicates selectively in Prostate cells, relying on a Prostate specific PSE-PBN promoter to generate a recombinant viral vector. CRADs can be used as a tool to detect circulating prostate tumour cells. CRADs expressing HSVTK could be a potential tool in imaging prostate tumours.

I was a part of the team that was awarded the patents for "Adenoviral fiber exchange shuttle system (Publication number: US 8088621 B2)" and "Compositions and methods for generating and screening adenoviral libraries (Publication number: US 200******** A1)".

I have worked on projects that are designed to study the regulatory role of small non-coding RNAs, microRNAs (miRNAs) in the disease progression of PCa, by analyzing its impact on gene expression and its influence on physiological effects in cell lines such as LNCaP, LAPC4, VCaP, & PC3. I was involved in studies that screened and profiled the miRNAs (miR890 and miR-774-3p) as the regulator of DNA Repair Pathway components in PCa, to develop Ionizing Radiation Sensitizing strategies as PCa therapeutics.

I work very diligently to maintain & operate laboratory instruments, equipments, order and organize research and lab supplies, to keep the lab in an efficiently operational mode.

I hope to have an opportunity to be interviewed for the position at hand.

EDUCATION:

June 1997- JOHNS HOPKINS UNIVERSITY

August 2000 Master of Science in BIOTECHNOLOGY

Courses include: Cell Biology; Cancer Biology; Biostatistics; Medical Virology; Human Molecular Genetics; Recombinant Protein Expression, Production and Analysis (Lab); Recombinant DNA (Lab); Computers in Molecular Biology.

January 1997- GEORGE WASHINGTON UNIVERSITY, WASHINGTON D.C.

May 1997 Advanced Biochemistry Course.

June1986- UNIVERSITY OF BOMBAY, INDIA

May 1988 Master of Science in BIOCHEMISTRY

June 1983- UNIVERSITY OF BOMBAY, INDIA

March 1986 Bachelor of Science in BIOCHEMISTRY / MICROBIOLOGY

EXPERIENCE:

I work productively and efficiently in performing Lab responsibilities and in implementing scientific projects assigned to me. I use theoretical knowledge in designing experiments, apply analytical skills in completing assigned projects and do trouble-shooting with respect to experiments and instruments, when needed.

April 2016- Johns Hopkins University, BioPhysics and BioPhysical Chemistry, Baltimore, MD.

November 2016 RESEARCH SPECIALIST

Following were my responsibilities in the lab:

Perform techniques in Molecular Cloning: LIC and SLIC Cloning using MacroLab Vectors.

Express E.coli proteins such as DnaA, LigaseA. using IPTG or Auto-induction. Analyze the expression by running SDS Protein Gels using Coomassie stain.

Site-directed Mutagenesis to produce protein mutants.

Monitoring and maintaining inventory of Lab & Research supplies. Place orders for these materials and those needed by lab individuals for their projects using the on-line SAP/SRM requisitioner. Receive & ship biological materials from various JHU affiliations both in the US & internationally, as per the DOT/IATA specifications.

Interact & Communicate with vendors for quotations, special pricing, and product related technical questions.

Use of On-Line resources such as Clustal Omega, Home Benchling, EcoCyc, UCSC Genome browser, IDT and NEB tools.

August 2004- Johns Hopkins University, Brady Urological Labs. Baltimore, MD.

March 2016 RESEARCH SPECIALIST

Following were my responsibilities in the lab:

Work with established molecular biology protocols for cloning plasmids carrying genes of interest to produce Recombinant Adenoviruses. Manipulate cell-lines for altering Gene-expression in cells.

Perform methods and techniques in Molecular Cloning: Aseptic Techniques, Use of Laminar Floor Hood, Cell-Culture, Isolation of Nucleic acids, Gel-electrophoresis, PCR, RT-PCR, & qPCR.

Isolation of Proteins, quantitation and Western blot analysis of proteins.

Perform functionality assays and do analysis of the data generated.

Instruct and assist Fellows and students, to facilitate problem solving in the Lab.

Familiar with Mice sub-cutaneous tumour implantation, surgeries and Luciferin Imaging.

Manage the General Laboratory maintenance, by scheduling Preventive maintenance for consistent and reliable performance of the lab equipments. Monitoring and maintaining inventory of Lab & Research supplies, & CO2 tanks. Place orders for these materials and those needed by lab individuals for their projects using the on-line SAP/SRM requisitioner. Receive & ship materials from various JHU affiliations both in the US & internationally as per the DOT/IATA specifications.

Operate & Maintain Lab equipments such as PCR, Real-time PCR Thermalcyclers, Fluorimeter, Luminometer & Liquid-Scintillation counter, Gel-imager, Centrifuges, pH-meter, Analytical balance, Spectrophotometer, Light and Fluorescent Microscopes, Biological Safety Cabinet, Incubators, Refrigerators, and -80 C &- 20 C Freezers, also handles CO2 tanks attatchment and operation.

Use the MESA electronic system to monitor Freezer & Incubator alarms.

Interact & Communicate with vendors for quotations, special pricing and product related technical questions.

Working knowledge of personal computers in the Windows environment: Microsoft office (MS word, Excel, Power point). Carry out tasks involved in connecting instruments with computers, downloading, and installing software. Responsible for operation of lab computers for data entry and retrieval.

RESEARCH PROJECTS and PUBLICATIONS:

A novel method for generating and screening peptides and libraries displayed on adenovirus fiber

pFEX: A Highly Efficient Adenoviral Vector System for Fiber Gene Modification

Adenovirus Targeting to Prostate-specific Membrane Antigen through Virus-displayed Semi-random Peptide Library Screening

miR-21: An Androgen Receptor-regulated microRNA that promotes Hormone-Dependent and Hormone Independent Prostate Cancer Growth

A Novel Approach for Detecting Viable and Tissue-Specific Circulating Tumor Cells through an Adenovirus-Based Reporter Vector

A Functional Screen Identifies miRNAs that Inhibit DNA Repair and Sensitize Prostate Cancer Cells To Ionizing Radiation

Investigation of miR21, miR41 and miR221 Expression levels in Prostate Adenocarcinoma for associated Risk of Recurrence after Radical Prostatectomy

Identification of miR-30b-3p and miR-30d-5p as direct regulators of Androgen Receptor Signaling in Prostate Cancer by complementary functional microRNA library screening

Involved in the following Projects:

HSVTK-PET Imaging Using Competent Replicating Adenovirus (CRAD) For Detection Of Prostate Cancer

January 2002- BIORELIANCE CORPORATION Rockville, MD.

June 2004 MEMBER OF THE TECHNICAL STAFF II

Carried out production of master cell-banks and working cell-banks of adherent and suspension Mammalian and Insect cells using T-flasks, Roller bottles, spinner and shaker flasks.

Following were my responsibilities at BioReliance: Use of Aseptic techniques and current Good Manufacturing Practice (cGMP).

Conducting GLP and cGMP compliant laboratory activities in Cell-banking and Virus-banking.

Prepare Media and samples for Mycoplasma and Sterility tests.

Non-Viable and Viable Cell-count by Haemocytometer,

Follow SOPs. Document Production Records and other Controlled forms as per cGMP.

Use of Inverted Microscope, Upright Microscope, Bench-top and Cold centrifuge, Controlled Rate Freezer, pipettes and pipettors.

I have also received hands-on training in:

Seeding, Infecting and Harvesting Cell-factories.

Purification of virus by CsCI gradient and Dialysis of virus using Cassettes.

October 1997- LAB SUPPORT: KIRKEGAARD & PERRY LABORATORIES Gaithersburg, MD

March 1998 QUALITY CONTROL LABORATORY TECHNICIAN

I gained experience in quality control of immunochemical products such as conjugated

secondary antibodies and proflok diagnostic kit reagents using ELISA methods.

Gained hands-on experience with microtitre plate and membrane ELISA, enzyme kinetics, protein quantitation and preparation of histology slides to study functionality of conjugated secondary antibodies.

Used UV/visual spectrophotometer, ELISA reader, Fluoroskan, pH-meter and Autoclave.

Gained familiarity with SOPs, QC paperwork and releasing products after quality control.

Worked with computer software packages namely Invitro cal.,Shimadzu, Microsoft Access and Fourth shift.

January 1991- RESEARCH ASSISTANT, HURKISONDAS NUROTTAMDAS MEDICAL

May 1992 RESEARCH SOCIETY, Mumbai.

Gained Hands-on experience with laboratory instruments namely balance, centrifuge, light microscope, spectrophotometer, platelet aggregometer, particle counter and autoanalyzer.

(TLC), SDS-PAGE, media preparation, whole blood and serum separation, staining procedures, blood counts, differential leukocyte counts, ESR and hematocrit.

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