Heydi Costales.

**** ** ***** ******* *** ***D Pembroke Pines. FL 33027

Cell: 786-***-****

acyg21@r.postjobfree.com

Summary

Highly Motivated microbiologist with experience in academic research laboratories. Particular expertise in cell biology and hematology. Always willing to learn new techniques and technologies Fluent in Spanish and English.

Highlights:

Real time PCR

PCR amplification

ELISA (sandwich and direct)

Northern,Southern and Western blot analysis

Flow cytometer (FC-500)

Vi-Cell (cell viability analyzer)

PBMC Isolation using Ficoll density gradient centrifugation

Expert cell culture and cryopreservation

Bacterial genetics

Proficient know of bacterial agents and standard chemical tests

Highly skilled in microbiology techniques, terminology and equipment and supplies.

Skilled professional at interpreting research outcome & leading research assignments

Exceptional organizational & problem-solving skills

Profound ability to prepare research reports for publications and white papers

Excellent computer skills combined with statistical skills

Hematology with three years of experience.

Microsoft Office (High complexity Excel Graphs,Pivot tables)

More than 5 years of experience in field of molecular biology and bacteriology

In depth knowledge of laboratory techniques and equipments.

Experience:

Consultant with Abbott Hematology Division. Santa Clara, CA, USA Current

Apliccations Scientist

Optimization of the Slide Maker Stainer (HSQm).

Integration HSQm with the HSQ analyzer (HSQa)

V&V procedures and report of the Slide HSQ.

Immunosite Technologies. Broward, FL. USA 2014 to current

Laboratory assistant. Applications Scientist

Lab inventory, cleaning, maintenance, calibration, and ordering chemicals and materials.

Following ISO 9001:2008 standards comply with QA/QC.

Participation and assisting to obtain ISO9001:2008 and ISO13485:2003 certifications

Experience in the operation, calibration and maintenance of laboratory equipment and analytical as Flow cytometer, Medonic M series (hematology analyzer), Vi-Cell (cell viability analyzer).

Analysis and design of experiments, interpretation of data, reports of results

Design and development of automated PBMC isolator

Design and development of automated blood fractionation and aliquoting system

Development, optimization, and automation of biological processes.

National Institute of Oceanology. Havana, Cuba 2009-2011

Researcher (Marine Microbial Ecology)

Validation and implementation of environmental quality indicators in beach areas taxing the National Environmental Monitoring System (A Case Study of Playas del Este).

Advances in strategies for adaptation to climate change to increase the resilience of coral Acrosporidos in Cuba, northern Yucatan and South Florida: Phase 1.

Environmental quality assessment models coastal marine ecosystems from microbiological and chemical indicators in the Zapata Peninsula APRM. Case Study of the GEF / UNDP'' Application of a regional approach to the management of coastal and marine protected areas in the islands south of Cuba''.

Biomonitoring of coral reefs between the Rivers Quibú and Baracoa, and test potential environmental indicators from sessile communities.

Genetic Engineering and Biotechnology Center. Havana, Cuba 2007-2009

Laboratory of Molecular Biology (Student’s Thesis) Molecular Oncology Project

Study of the mechanism of action of pro-apoptotic peptide CIGB-300 cell line SIHA cervical cancer.

Education:

2009 Biology Faculty at University of Havana. Havana, Cuba

Bachelor of Science in Microbiology

Postgraduate Courses:

Basic training in safety, IMO, Cuba 2011.

Proficiency in survival craft and rescue boats other than rapids. OMI rescue boats, Cuba 2011.

Highlights for the protection and sustainable use of coral reefs. IDO, CITMA. Cuba 2010.

Theoretical and practical application of the evaluation matrix RIAM environmental impact. IDO, CITMA. Cuba 2010 / Scuba diving (1 star). IDO. CITMA. CMAS, Cuba.

Water management in CESPANEC in collaboration with the University of Alicante, Spain. Cuba. 2009.

Publications:

Yasser Perea, Heydi C.Costales, Yakelin Diaz, Osvaldo Reyes, Hernan G. Farina, Lissandra Mendez, Roberto E. Gomez, Boris E. Ecevedo, Daniel E. Gomez, Daniel F. Alonso and Silvio E. Perea. Sensivity of tumor cells towards CIGB-300 anticancer peptide relies on its nucleolus localization. Journal of Peptide Science. 2012, 18: 215-223.

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