Blake Cherney
**** ****** ** ** *******, GA, ****8
***********@*****.***
Education
Georgia State University, College of Arts and Sciences
M.S. Biology (Applied Environmental Microbiology) May 2015
Bachelor of Science/Biology/Minor Chemistry May 2013
Work Experience
Molecular Microbiologist, Center of Disease Control and Prevention October 2015 – Present
Atlanta, Georgia
Biodefense Research and Development Laboratory (BDRD)
oDeveloped assays for Rapid Antimicrobial Susceptibility Test (RAST) for biothreats agents (Bacillus anthracis, Yersinia pestis, Burkholderia pseudomallei, Burkholderia mallei, and Francisella tularensis)
oResponsible for all chemical waste, and recycling of all expired chemicals
Proficient in various sequencing technologies
oWhole Genome Sequencing with Illuminia Miseq, and MinIon
Prepared large amounts of DNA for Pac-Bio sequencing
oSequenced hundreds of 16s rRNA genes for identification to the Department of Homeland security
Submitted monthly reports to DHS
Created reports for each sample with BLAST and RDP results.
Legal Field Assurance Specialist, C. R. Bard, Inc June 2015 – October 2015
Covington, Georgia
Executed good manufacturing practices (GMPs), in compliance with Federal Regulations
Evaluated complaints to determine Medical Device Reportable (MDR) events
oPerform final review for closure of complaint files to ensure accuracy, completeness, attention to detail, and file closures on all domestic and international complaints.
oFiled Report to FDA within 30 days of inquiry
Ran several recalls on medical devices
oLeading a team of specialist to contact customers that received recalled products
oCoordinated with quality control managers, and technicians
Quality Control Technician, SweetWater Brewing Company June 2014 – September 2014
Atlanta, Georgia
Followed environmental and safety regulations in compliance with US law.
Developed and implemented new projects and microbiological procedures in lab
oDeveloped new media for early detection of Bacillus spp.
oBottle conditioned tanks for production
Monitored and measured
oDissolved oxygen, CO2, IBU, and alcohol percentages,
oPathogen testing
oYeast propagation, and quantification
oReleased beer for packaging
Graduate Teacher, Georgia State University January 2013 – May 2015
Atlanta, Georgia
Taught Introduction to Microbiology lab to biological sciences and nursing, public health and biology majors
oSimple and Gram staining
oLaboratory safety
oPublic health instruction
oBroth dilution
oThermal death time and thermal death point experiments
oMicroscopy
oBacterial isolation
oAntimicrobial susceptibility testing
Blake Cherney, M.S.
Molecular Basis of Disease Research Fellow
Atlanta, Georgia May 2013 – August 2013
Performed research on the symbiotic relationship of Acanthamoeba and other pathogenic organisms under Donald G Ahearn PhD
Contributed to development of biological control agents for White-Nose Syndrome in bats with Chris Cornelison PhD
oCultured Pseudogymnoascus destructans and Rhodococcus rhodochrous
Completed semester reports for the Biology department chair, Dr. Sidney Crow PhD
Introduced research speakers for the Molecular Basis of Disease research conference
Research Experience
Graduate Research on Snake Fungal Disease caused by Ophidiomyces ophiodiicola: August 2010 – May2015
Researched the growth effects of several volatile organic compounds in a closed air space.
oConverted results to statistical data in excel
oFacilitated using Photoshop (Gimp 2.0) to measure growth of fungal plug assays
oPresented research at Georgia Bio Innovation Summit 2014
Worked with Zoo Atlanta to identify O. ophiodiicola using Quantitative PCR
oSupervised and organized projects Zoo Atlanta and was responsible for a small class of researchers
oDirected and delegated tasks to efficiently handle large quantities of samples
oMentored graduate students and trained undergraduate researchers to use basic laboratory techniques
Undergraduate and Graduate Research on Acanthamoeba castellanii: August 2012 – August 2015
Worked in an applied environmental microbiology lab at Georgia State University under Sidney Crow PhD
Performed intermediate lab procedures
oMedia preparation
oStreaking and isolation methods
oBacterial cultures
oStaining and microscopy
oSpore harvesting
oHemocytometer quantification
oAcanthamoeba culturing methods
oAir sampling and public health effects of air quality.
Research in International Genetically Engineered Machine (IGEM) January 2013 – June 2013
Performed molecular techniques under Mathew Brewer PhD
oPerformed molecular analysis including DNA and plasmid extractions
oPCR and gel electrophoresis
oRestriction digest
oPrepared competent cells
oBacterial transformation
oPrepared glycerol stocks
Publications
Cornelison, Christopher T., Blake Cherney, Kyle T. Gabriel, Courtney K. Barlament, and Sidney A. Crow Jr. 2016. Contact-Independent Antagonism of Ophidiomyces ophiodiicola, the Causative Agent of Snake Fungal Disease by Rhodococcus rhodochrous DAP 96253 and Select Volatile Organic Compounds. Journal of Veterinary Science & Technology 7.
Bugrysheva, Julia V., Blake Cherney, David Sue, Andrew B. Conley, Lori A. Rowe, Kristen M. Knipe, Michael A. Frace et al. "Complete genome sequences for three chromosomes of the Burkholderia stabilis type strain (ATCC BAA-67)." Genome Announcements 4, no. 6 (2016): e01294-16.
Blake Cherney, M.S.
Conferences & Presentation
European Congress of Clinical Microbiology & Infectious Diseases- Poster pres. April 2017
Vienna, Austria
Georgia Bio Life Sciences Summit- Poster pres. October 2014
Atlanta, Georgia
References
Dr. Linda Weigel Chris Cornelison
Laboratory Supervisor Post-Doctoral Research Mentor
Principle Investigator Principle Investigator
Biodefense Research and Development Laboratory Division of Research and Advanced Study
Centers for Disease Control and Prevention Kennesaw State University
Email: *.*******@*****.*** Email: ********@********.***
Telephone: +1-404-***-**** Telephone: +1-404-***-****
Dr. David Sue Sidney Crow PhD
Laboratory Supervisor Laboratory Supervisor
Acting Principle Investigator Principle Investigator
Biodefense Research and Development Laboratory Applied Environmental Microbiology
Centers for Disease Control and Prevention Georgia State University
Email: ****@***.*** Email: *****@***.***
Telephone: +1-404-***-**** Telephone: +1-404-***-****