Medical Clinical Research

Nicole Monique Frilot

***** ******** **.

Lake Forest, CA 92630

Home: 949-***-****

Cell: 706-***-****

**********@*******.***

EDUCATION:

Augusta University

Augusta, GA

B.S. in Biology May 2002

EMPLOYMENT HISTORY:

Molecular Biology Technologist

Oct. 2014 to March 2016

Microbiology Laboratory

Augusta University Medical Center

Augusta, GA

Deanna Craig, SM, MT (ASCP)

Helping to introduce faster molecular approaches to the microbiology laboratory

1. Perform and maintain all molecular assays QC documentation, and troubleshoot all aspects of assays when needed. Enter all patient and QC data into LIS system.

2. Perform and write up validations, laboratory procedures, and IQCPs on the molecular assays introduced into the lab, including Cepheid GeneXpert Infinity Sytem (MRSA, C. diff, FII/FVL, EV, and MTBR), BioFire FilmArray (Respiratory Pathogens, Gastrointestinal, and Meningitis Panels), T2 Biosystems T2 Candida Panel, Nanospehere’s Verigene System (Gram Positive and Gram Negative Bacterial Panels), and BioMerieux’s resin filled blood culture bottles.

3. Perform routine microbiology and molecular assays: All the ones stated above, Abbott’s M2000 (HIV viral load and CT/NG), specimen plating, and slide reading.

Laboratory Assistant/ Associate

March 2003 to Oct. 2014

Augusta University (3 different labs)

Augusta, GA

I.Dr. Ravindra Kolhe, MD, PhD

Viral & Genetic Clinical Testing in the Georgia Esoteric and Molecular Laboratory, LLC (clinical research lab)

1. Maintain all QC records and perform troubleshooting when necessary. Enter all patient and QC data into LIS system.

2. HIV-1 & HCV quantitative viral load RT PCR analysis using Roche’s COBAS Ampliprep/Taqman. CMV quantitative viral load real time analysis using Roche’s Light Cycler and Bio-Rad for gel fragmentation analysis.

3. Sequencing: HIV-1 drug resistance & cystic fibrosis mutation analysis using Applied Biosystem’s 3100 Genetic Analyzer.

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II.Dr. Yehia Daaka, PhD

Role of G-protein coupled receptors in cancer progression. (academic research)

1. Injection of mouse bladders with E. coli and NOS inhibitors to find the role of Dynamin II, a GTPase involved in endocytosis, and eNOS, an endothelial nitric oxide synthase, in urinary tract infections. The bladders were prepared for further studies such as agar growth colony count,HPLC, immunofluorescence, and immunorhistochemistry.

2. To discover a correlation between Rap1GAP, a RAP1 GTPase activating protein, and prostaglandin E Receptor 4 (EP4) in the metastatic potential of kidney cancer. Many in vitro and in vivo experiments were performed to achieve this goal, such as cell transfections, cell invasions using transwell plates, cAMP assay, Flow Cytometry, cell proliferation assay, and western blotting.

3. Maintenance of multiple cell lines to facilitate in vitro experiments performed by all lab personnel.

III.Dr. Stephen Peiper, MD

Role of CXCR4 & CCR5 in breast cancer metastasis and HIV entry into cells (academic research)

1. GST purification of different domains of the G protein coupled receptor CXCR4 to identify target binding proteins using a GST pulldown assays, cell migration assays, flow cytometry analysis, calcium flux, GTP binding.

2. Using mouse models to help understand the involvement of CXCR4 and CCR5 in breast cancer metastasis.

3. Preservation of lab aesthetics with focus on cleanliness, organization, and maintenance of stock chemicals and reagents

PUBLICATIONS:

Zhang W, Navenot JM, Frilot NM, Fujii N, Peiper SC. Association of nucleophosmin negatively regulates CXCR4-mediated G protein activation and chemotaxis. Mol Pharmacol. 2007 Nov;72(5):1310-21.

Kim JI, Lakshmikanthan V, Frilot N, Daaka Y. Prostaglandin E2 promotes lung cancer cell migration via EP4-betaArrestin1-c-Src signalsome. Mol Cancer Res. 2010 Apr;8(4):569-77.

Wang Z, Humphrey C, Frilot N, Wang G, Nie Z, Moniri NH, Daaka Y.

Dynamin2- and endothelial nitric oxide synthase-regulated invasion of bladder epithelial cells by uropathogenic Escherichia coli. J Cell Biol. 2011 Jan 10; 192(1): 101-10.

Wu J, Zhang Y, Frilot N, Kim JI, Kim WJ, Daaka Y. Prostaglandin E2 regulates renal cell carcinoma invasion through the EP4 receptor-Rap GTPase signal transduction pathway. J Biol Chem. 2011 Sep 30; 286(39): 33954-62.

Wang Z, Kim J, Frilot N, Daaka Y. Dynamin2 S-nitrosylation regulates adenovirus type 5 infection of epithelial cells. J Gen Virol. 2012 Jul 12.

References:

Deanna Craig – Microbiology Supervisor @ AU Medical Center – 706-***-****

Kellie Foss – Administrative Director of Pathology @ AU Medical Center – 706-***-****

Dr. Jae Kim – Medical Resident @ Georgetown University - 770-***-****

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