Technical Support Microsoft Office
Resume:
************@*****.***
Research Associate/Research Scientist
MS in Biotechnology with competent knowledge of Biotechnology, Neuroscience, Protein purification and Expression, and Pharmacy seeking position like Research Associate in Pharmaceutical or Biopharmaceutical company.
Summary
Work with our Pharmacokinetic/Pharmacodynamics (PK/PD) scientists and partners to deliver capabilities to business clients in our clinical business areas
PCR, Reverse transcription PCR, Real time PCR, in vitro transcription, RNA purification, SELEX, Nucleic acid quantification
Electrophoresis including Agarose gel electrophoresis, SDS-PAGE, Native PAGE, Western blotting, Isoelectrofocussing, Densitometric analysis, Filter retardation assay, Lyophilization
Protein purification and analysis techniques- HPLC, FPLC, Affinity Chromatography (Ni-NTA and Glutathione Agarose matrix), Size exclusion chromatography, Ion exchange chromatography, Hydrophobic interaction chromatography, UPLC (waters). Mass spectrometry (AB SCIEX and Waters)
ELISA (Enzyme Linked Immunosorbent Assay), Mammalian cell lines handling. Particle Size analysis, Karl Fischer, Accelerated Stability Study
Hands on protein purification instruments like AKTA Pilot, AKTA purifier, AKTA explorer
Spectroscopic techniques: UV-Visible Spectroscopy, Colorimetery, Luminance Spectroscopy
Formulation of Liposomes
Good understanding of Biostatistics, Economic Analysis, Financial Accounting, Principal of finance and Corporate Finance, health care policy, Society/Ethics/Legal Environment of Business, Structure and Regulation of Pharmaceutical Industry
Document and verify business process flows and business requirements.
Responsible for documenting system operating procedures, Immunology
Keep abreast of new technologies, methodologies, industry practices and standards
Ability to perform tasks cooperatively in team environment as well as independently
Corporate Skill
Downstream processing of Biomolecules Market Research Spectroscopic techniques
HPLC Affinity Chromatography RNA and DNA Isolation Techniques of Protein Purification Polymerase chain reaction qPCR
FDA regulation Stability testing Formulation of Liposome
Stability testing Lyophilization Colorimetry
AKTA Purifier Ultrafiltration Flow Cytometry
Software Skill
Proficiency with SPSS, SAS, Salesforce, SQL, PROC SQL, Microsoft Office (Word, Excel, PowerPoint), Chemdraw, Chemsketch, Reference Management Software (Endnote Plus). Scientific data retrieval from various internet portals like Science direct, Google scholar, PubMed, Scirus.
Education
MBA in Pharmaceutical Management (December 2016)
Fairleigh Dickinson University, Teaneck, NJ GPA 3.7
Master of Science Specialization in Biotechnology (MS) (June 2012 to June 2014)
National Institute of Pharmaceutical Education and Research, Chandigarh, Punjab, India GPA 4.0
Bachelor of Pharmacy (June 2008 to June 2012)
Gujarat Technological University, Vadodara, Gujarat, India GPA 4.0
Professional Experience
Pharma Business Analyst Integra Technology Ltd., Delaware, USA Oct 2016- Dec 2016
Develop an understanding of our customer facing teams needs and ensure our platforms deliver immediate and long term capabilities
Drive business process design through the application of Six Sigma and other operational excellence techniques.
Research Associate Intas Biopharmaceutical Ltd., Ahmedabad, India June 2014 – December 2014
Handling project in Drug product development department of biosimilar department at Intas Biopharmaceutical Department.
Downstream Process Development and Protein Purification for Microbial and Mammalian Products
Provide technical support for designing experiments and facilitating technology transfer activity to manufacturing
To ensure timely reporting of deviations, investigation & resolution of manufacturing discrepancies, Process optimization, Tech transfer, validation & documentation at lab and production scale, process monitoring, data entry, data analysis and data presentation.
Excellent management of day to day process activities in all shifts and personnel management.
Identify process improvements opportunities & implement the process changes through QA systems.
Research Scholar National Institute of Pharmaceutical Education and Research, Chandigarh, India
June 2012 to June 2014
Completed project on “Effect of aptamers on the function of mutant huntingtin protein”.
Aptamers are single stranded deoxyribonucleic acid (ssDNA) or ribonucleic acid (RNA) or peptide molecules that can bind to the target with high affinity and specificity due to their three dimensional structures.
Plasmid isolation by midi prep method.
Restriction digestion was performed to check the integrity of the insert in the plasmid.
In vitro transcription of plasmid DNA, RNA purification and Quantification
Optimize condition for in vitro aggregation of mutant huntingtin protein
Thioflavin T assay to study the aggregation of protein
Reactive oxidative stress study
Liposome was prepared and the formation of pores by mutant huntingtin will be measured by leakage of flourescein dye from the interior of liposome.
Internships
Macro Pharmaceuticals Healthcare, India (Training Associate – December 2011): - 1 Month internship in QA & QC, Production, Packaging and storage department
Amoli Organics Ltd, India (Training Associate – December 2010): 1 Month training experience in Corporate QA & QC department
Research Project
“Effect of aptamers on the function of mutant huntingtin protein” In this work aptamers were selected against the mhtt that specifically inhibit aggregation of mhtt. Ability of aptamers to prevent pore formation by mhtt was checked by monitoring leakage of dye incorporated in liposome.
Publication and Presentation
Chaudhary, R. K., Patel, K. A., Patel, M. K., Joshi, R. H., & Roy, I. (2015). Inhibition of Aggregation of Mutant Huntingtin by Nucleic Acid Aptamers In Vitro and in a Yeast Model of Huntington’s disease. Molecular Therapy (DOI: 10.1038/mt.2015.157)
Presented a poster entitled " RNA aptamers as potential inhibitors of aggregation-induced membrane permeabilization" at 82nd Annual Meeting of Society of Biological Chemists, India and International conference on genomes: Mechanism and Function Dec, 02-05, 2013
Honor and Awards
2nd rank in NIPER, India with 9.6 CPI
5th rank in University of Gujarat, India during Bachelor of Pharmacy
Professional Membership
Certified Pharmacist in India by Pharmacy Council of India from 2012 to Present
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