Weibo Li

*** **** ******* **, ******, MA *****; 508-***-****(C); Email:acxzzv@r.postjobfree.com

Experience:

May, 2005--- April, 2016. Research Technician, Dr. Nir Hacohen’s lab, MGH, Center for Immunology and Inflammatory Disease, and Cancer Center

. Screening interesting genes in human and mouse lentiviral shRNA libraries.

. Identification of TLRs and other pathogen sensors specificity in their induction of gene expression.

. Culture of dendritic cells (DC) from human PBMC isolation by Ficoll and beads.

.Mouse tail DNA extraction and genotyping.

.Vector construction and genomic DNA indel identification of gene edition by CRISPR-CAS9 system.

May 5, 2003 – May8, 2005, Research Associate. Dr. Lucio Castilla’s lab, Program in Gene Function & Expression, UMASS Medical School

. Identifications of novel Cbfb-MYH11 gene partner genes in leukemia by retrovirus integration mutagenesis (RIM) strategy.

.Amplification of CBFβ-MYH11 fusion gene partners by inverse PCR and sequencing; Extraction of DNA and RNA from mouse tissues; inverse PCR; cloning; preparing Competent Cells; genotyping analysis for knock-in mouse tail and ear, FACS analysis, etc.

Sept. 2001– May 2. 2003, Lab Technician. Dr. Szabo’s lab, Medicine Dept., UMASS Medical School.

. The role of heme-oxygenase in alcohol mediated cytokineregulation.

.Upregulation of MD-2 in LPS hypersensitive livers in mice, etc.

LABORATORY SKILLS

Immunology:

ELISA; PBMC,T cells and dendritic cells isolation and culture from human peripheral blood by beads and Ficoll; antibody staining, Western blotting; Flow cytometry operation and analysis; Flow Jo software use;

Cellular Biology and Cell-Based Assays:

Construction of retroviral and lentivirus vector and production of retrovirus and lentivirus; High efficiency lentivirus and retrovirus infection; primary cell and cell line culture; Cell viability, proliferation and apoptosis assay; reporter gene assays;

Molecular Biology and Biochemistry:

DNA manipulations(cloning, sequencing); RT-PCR and QPCR; Nanostring, Crispr-Cas9 system sgRNA.

Generation of knock-out and knock-in mouse maintains and evaluation of gene functions in vivo efficacy:

Genotyping and function analysis of knock-out, knock-in and transgenic mouse.

Collect blood and organs cells of animals tissues for cell studies and ex vivo assays

Education and Degrees:

BS, Chemistry, East China University of Chemical Technology, Shanghai, China, 1991.

BBA, International Trade, Shanghai Institute of Foreign Trade, Shanghai,China, 1998

Publications :

1.Lan YY, Londoño D, Bouley R, Rooney MS, Hacohen N .Dnase2a deficiency uncovers lysosomal clearance of damaged nuclear DNA via autophagy.Cell Report. 2014 Oct 9; 9(1):180-92 . ( We thank S. Nagata and K. Kawane (Kyoto University) for Dnase2a-deficient mice; R. Xavier for Atg5 / MEFs; F. Marangoni, F. Castelino, and N. Sakai for technical advice; W. Li, M. Roy, and M. Lee for technical help and reagents

2. Common genetic variants that modulate pathogen-sensing in human dendritic cells

Mark N. Lee, Chun Ye, Alexandra-Chloé Villani, Towfique Raj, Weibo Li, Thomas M. Eisenhaure, Selina H. Imboywa, Portia I. Chipendo, F. Ann Ran, Kamil Slowikowski, Lucas D. Ward, Khadir Raddassi, Cristin McCabe, Michelle H. Lee, Irene Wood, Manolis Kellis, Soumya Raychaudhuri, Feng Zhang, Barbara E. Stranger, Christophe O. Benoist, Philip De Jager, Aviv Regev, Nir Hacohen. Science. 2014 Mar 7; 343(6175):1246980.

3. Identification of regulators of the innate immune response to cytosolic DNA and retroviral infection by an integrative approach. Lee MN, Roy M, Ong SE, Mertins P, Villani AC, Li W, Dotiwala F, Sen J, Doench JG, Orzalli MH, Kramnik I, Knipe DM, Lieberman J, Carr SA, Hacohen N. Nat Immunol. 2013 Feb; 14(2):179-85.

4. IAP inhibitors enhance co-stimulation to promote tumor immunity. Dougan M, Dougan S, Slisz J, Firestone B, Vanneman M, Draganov D, Goyal G, Li W, Neuberg D, Blumberg R, Hacohen N, Porter D, Zawel L, Dranoff G. J Exp Med. 2010 Sept. 207(10):2195-206

5. Unbiased reconstruction of a mammalian transcriptional network mediating pathogen responses.Amit I, Garber M, Chevrier N, Leite AP, Donner Y, Eisenhaure T, Guttman M, Grenier JK, Li W, Zuk O, Schubert LA, Birditt B, Shay T, Goren A, Zhang X, Smith Z, Deering R, McDonald RC, Cabili M, Bernstein BE, Rinn JL, Meissner A, Root DE, Hacohen N, Regev A. Science 2009, 326: 257-63.

6. Cbf beta-SMMHC induces distinct abnormal myeloid progenitors able to develop acute myeloid leukemia.Kuo YH, Landrette SF, Heilman SA, Perrat PN, Garrett L, Liu PP, Le Beau MM, Kogan SC, Castilla LH. Acknowledgements: The authors wish to thank Richard Konz,Jr., Weibo Li, and Mei lin Maunakea for their technical assistance Cancer Cell. 2006 Jan; 9(1):57-68.

7. Plag1 and Plad12 are oncogenes that induce acute myeloid leukemia in cooperation with cbfb-MYH11. Sean F Landrette,Ya-Huei Kuo, Karen Hensen,Barjesteh Khosrovani,Paola N Perrat, Wim JM Van de Ven, Ruund Dewel, Lucio H Castilla. Acknowledgements: We wish to thank Michelle Kelliher critical reading of the manuscript, and Weibo Li and CJ Clark for technical assistance Blood 2005, 105(7):2900-2907

8. Heme oxygenase-1 mediates the anti-inflammatory effects of acute alcohol on IL-10 induction involving p38 MARK activation in monocytes. Drechsler Y, Dolganiue A, Norkina O, Romics L, Li W, Kodys K, Bach FH, Mandrekar P, Szabo G, J Immunol. 2006 Aug; 177(4):2592-600.

9. Upregulation of MD-2 in LPS Hypersensitive livers in mice. Romics L Jr. Velayudham A, Dolganiuc A, Li W, Szabo G. Hepatology and Liver Center, University of Massachusetts Medical School, Worcester, MA01655, Irish Society of Immunology Annual Scientific Meeting, Sept. 18-19,2003.

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