THOMAS H. BRODY

**** ************ ******, ********* ** Brighton, MA 02135

617-***-**** acxz0z@r.postjobfree.com

LABORATORY TECHNICIAN

Delivering believable, dependable, clean results

RESEARCH DATA ANALYSIS INTERPRETATION MEASUREMENT QUALITY CONTROL LIBRARY SCREENING PLASMID PREPS SEQUENCING REACTIONS PIPETTING RNA DNA PCR TISSUE CULTURE CELL AND TISSUE PROCESSING LABORATORY PROCEDURES Diligent laboratory technician with extensive experience in multiple laboratory milieus and studies supporting disease and genetic research. Competent in laboratory protocol with a strong ability to ensure safety, exercise stringent study control and properly document expected and unexpected results. Skilled problem solver able to introduce new and improve existing procedures. Strong ability to identify anomalies, confirm results and determine course of action. TECHNICAL PROFICIENCIES AND EQUIPMENT

Radiofrequency Ablations

Wet Lab Techniques

DNA Sequencing and

Quantitation

PCR High-Throughput

Sequencing Chemistries

Alternative Sequencing

DNA-Spotting Robots

Microarray

Gel Pouring and Loading

Northern Blots

Dot Blot Hybridization

Radioactive Probe Synthesis

Densitometry

Plasmid Inserts

Genomic Libraries

Mixed Lymphocyte Assay

Spectrophotometer

Centrifuge

Photographic Imaging

RNA Isolation

LABORATORY EXPERIENCE

SUMMARY:

Supported research and data collection for various biological study projects throughout career. Collected (as needed) and processed tissue samples according to pre-defined protocol. Prepared samples, set-up tests and performed scientific research and analysis. Collated data, validated results and forwarded findings to lead researcher in support of study goals. Operated and calibrated laboratory and testing equipment. Produced dependable, repeatable results. Conducted peripheral tests, as requested, to test various hypotheses. Presented results to colleagues, supporters and study staff. SPECIFICS:

Beth Israel Deaconess Hospital

Research Assistant, Boston, MA 2005-2008

Participated in radiofrequency ablations study focusing on method to optimize clinical procedures to heat and destroy surgically inoperable hepatic tumors.

Protocols: radiofrequency ablations, bovine liver dissections, lesion measurements, cell cultures, Professional Sabbatical, Boston, MA 2000-2005

Took advantage of personal investment windfall and pursued personal interests during a period of discovery. Reentered workforce revitalized and centered.

Millennium Pharmaceuticals,

Research Associate, Cambridge, MA 1994-2000

Operated as part of automated DNA sequencing, microarray/PCR team for laboratory. Protocols: wet lab techniques, high-throughput and alternative sequencing, DNA-spotting robots. Harvard Medical School,

Research Associate, Boston, MA 1992-1994

Conducted all phases of sequencing: library screenings, plasmid preps, DNA quantitation, sequencing reactions, gel pouring and loading.

Protocols: ABI 373A Automated DNA Sequencer

THOMAS H. BRODY

PAGE 2 617-***-**** acxz0z@r.postjobfree.com

LABORATORY EXPERIENCE, CONTINUED

EARLY EXPERIENCE:

New England Medical Center

Research Associate, Boston, MA 1991-1992

Quantitated DNA using dot blots for professor’s on-going study. Protocols: Northern blot, dot blot hybridization, radioactive probe synthesis, densitometry, tissue culture. St. Elizabeth’s Hospital

Research Assistant, Boston Ma

Subcloned plasmid inserts in support of DNA research for human gene analysis project. Protocols: manual DNA sequencing, genomic library screening University of Connecticut

Research Assistant, Storrs, CT

Worked in cellular immunology laboratory. Examined effect of hyperthermia on cytotoxic T-cell mediated cell lysis and DNA synthesis in in vitro splenocyte populations.

Protocols: murine mixed lymphocyte assay,

ADDITIONAL EXPERIENCE

United Security, Inc

Security Guard, Financial District, Boston, MA 2010-Present Controlled access to 25-story, two-tower office building in downtown Boston housing multiple financial and communications firms, employing more than 1200 people. Verified appointments and directed visitors as part of first line of defense for high-profile, potentially targeted building. Otterbase, Inc.

Service Laborer, assigned to Logan Airport, East Boston, MA 2008-2010 Processed 500-1500 traveler bags per shift (depending on daily air traffic) as part of a two-person team. Maintained smooth flow of luggage, preventing delays and supporting effort to keep flights on schedule. Adhered to strict Homeland Security and Transportation Security Authority (TSA) regulations. EDUCATION

University of Connecticut, Storrs, CT

Master of Science (MS) Biology

Wesleyan University, Middletown, CT

Bachelor of Arts (BA) Biology

NOTABLES

Harvard Extension School, Boston, MA

Organizational Behavior Class 2010

Multiple Publications (See publications supplement)

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