Bryce Vincent, PhD

*** ******* ***** ***: 832-***-****

Houston, Texas 77007 e-mail: acxyph@r.postjobfree.com SUMMARY

Biomedical professional with experience in the review and interpretation of biomedical study data for classifying chemical safety, and research use. Broad range of experience in cross-field studies through the molecular and cellular analyses of signal transduction and hormonal pathways. Working to increase my knowledge and skills as a Biomedical professional in the applications of Biotechnology, Clinical Research, Neuroscience, Biochemistry, Endocrinology, Immunology, Molecular Biology, and Cancer Biology. Strong written and oral communication skills, supervisory experience, and computer skills. EXPERIENCE

Experien Health Sciences, Inc. 2013 - present

Scientific Consultant (for industry clients)

Review toxicological study data to determine UN GHS classification of chemical substances for a global leader in the distribution of chemicals and plastics. This client distributes over 27,000 products and has partnered with over 1,300 world-class chemical suppliers.

Experienced in reviewing EU REACH dossiers in the IUCLID 5 format.

Trained in the authoring of Safety Data Sheets according to UN GHS criteria for substances and mixtures.

Familiar with OECD Guidelines for the health effects, testing of chemicals, and the basic data requirements associated with registration / notification of new chemical substances under EU REACH, Canada NSN, and US TSCA PMN regulations.

Black Silver Development, Inc. 2011 - 2013

Scientific Consultant (for industry clients)

Conducted toxicological and scientific reviews of study data for the composition of scientific reports and manuals.

Composed and edited financial spreadsheets, general correspondence, direct mailings, scientific reports, manuals, and other documents.

Developed and maintained customer relationships through lead follow-up and client outreach programs. University of Texas Health Science Center at Houston MD Anderson Cancer Center (Houston, TX) 2008 – 2011 Graduate Research Assistant (laboratory of Dr. Zhengxin Wang)

Discovered that the androgen receptor (AR) cofactor p44/WDR77 can regulate astrogliosis (a major pathology in aging-related neurodegenerative disorders) by down regulating the expression of a cell cycle regulator, and the repression of the activation of the canonical nuclear factor kappa B (NF- B) pathway.

Using immunohistochemistry analysis of cell cycle and inflammatory pathways, demonstrated the molecular mechanism of the AR cofactor’s role in astrogliosis, which lead to a publication.

Developed stable astrocyte cell lines (with and without the p44/WDR77 gene), still in use in the field today, from primary mouse astrocytes for molecular analysis of astrocyte activation, and future in vivo carcinogenic analyses.

Mentored high school students, undergraduates, and a visiting foreign graduate student during execution of their assigned projects.

University of Texas Dental Branch (Houston, TX) 2005 – 2008 Graduate Research Assistant (laboratory of Dr. John Klein)

Discovered an in-frame, novel, immune-derived thyroid-stimulating hormone beta (TSHβ) splice variant utilizing 5' rapid amplification of cDNA ends (RACE) of mouse bone marrow (BM) cells, which lead to a publication, a patent application, and a new line of research.

Generated and isolated recombinant splice variant TSHβ protein to illustrate its ability to elicit a cAMP response from FRTL-5 thyroid follicular cells and a mouse alveolar macrophage (AM) cell line, characterizing the first functional splice variant of TSHβ, which can contribute to the metabolic regulation during immunological stress, and offers a new perspective for understanding autoimmune thyroiditis. Baylor College of Medicine (Houston, TX) 2004 – 2005 Research Coordinator (laboratory of Dr. Timothy Boone and Dr. George Somogyi)

Examined pharmacologic plasticity of muscarinic, and nicotinic receptors cross-reactivity in rat bladder and urethra urothelium through the analysis of the released nitric oxide (NO), Adenosine-5'-triphosphate (ATP) in organ chambers.

Demonstrated a correlation between increased ATP and adenosine concentration, and the severity of interstitial cystitis through high-performance liquid chromatography of patient samples Pediatric Neurology Clinic, UT Health (Houston, TX) May – August 2002 Summer Intern

Shadowed pediatric neurologist during clinical rounds and participated in the analysis of ongoing clinical research. EXPERIMENTAL APPROACHES

Biochemistry: Detergent extraction of intracellular and nuclear proteins; western blot SDS PAGE; ELISA; enzyme assays; immunoprecipitation; ELISA; recombinant protein isolation; HPLC Molecular Biology: Subcloning; recombinant DNA cloning; PCR; RT-PCR, nucleic acid isolation; RACE analysis Cell Biology: Maintenance and generation of mammalian cell lines from primary cultures; bacterial cell culture; immunocytochemistry; transient transfection; flow cytometry (FAC) Other: Small animal surgeries; microdissections; tissue harvesting; Ussing chamber; fluorescence spectroscopy; fluorescence microscopy

EDUCATION

Ph.D. Biomedical Sciences 2005 - 2011

University of Texas Graduate School of Biomedical Sciences and The University of Texas MD Anderson Cancer Center Advisor: Zhengxin Wang

Dissertation Title: The Role of the Androgen Receptor Cofactor p44/WDR77 in Astrocyte Activation B.S. Biochemistry 1998 - 2003

Schreiner University GPA - 3.90

AWARDS

2009 Endocrine Society Travel Grant 2009

1st Prize 2008 UTHSC-H Annual Research Day Award for Outstanding Poster Presentations 2008

GSBS Student Travel Award 2008

UT TORCH grant, University of Texas Dental Branch 2006 – 2008

AUA/Yamanouchi Best Abstract in the category of Incontinence 2005 Salas NA, Somogyi GT, Vincent BH, Boone TB, Smith CP. Effect of botulinum toxin A on urethral outlet resistance by measuring changes in leak point pressure.

President’s List for ten semesters at Schreiner University 1998 – 2003

Magna Cum Laude, Schreiner University, Kerrville, Texas 2003

Inducted into Kappa Mu Epsilon, a national math honor society 2003 Inducted into Alpha Chi, a national honor society for college juniors and seniors 2001 Inducted into Alpha Lambda Delta, a national honor society for college freshman 1999

Awarded Presidential Academic Award in 1998 by President Clinton 1998 PATENTS

Patent Office: United States

Patent/Application Number: 2011/0052591

Patent Title: Variants of Thyroid Stimulating Hormone Beta Inventor(s): John Klein, Jeremy Schaefer, Bryce Vincent PUBLICATIONS

Vincent B, Hong Wu H, Gao S, and Wang Z. Loss of the androgen receptor cofactor p44/WDR77 induces astrogliosis. Mol Cell Biol. 2012 Sep;32(17):3500-12

Vincent BH, Montufar-Solis D, Teng BB, Amendt BA, Schaefer J, Klein JR. Bone marrow cells produce a novel TSHbeta splice variant that is upregulated in the thyroid following systemic virus infection. Genes Immun. 2009 Jan;10(1):18-26. Varghese S, Montufar-Solis D, Vincent BH, Klein JR. Virus infection activates thyroid stimulating hormone synthesis in intestinal epithelial cells. J Cell Biochem. 2008 Sept;105(1):271-276. ABSTRACTS

Vincent B, Gao S, Wang Z: The role of Androgen receptor cofactor p44 in mouse brain aging. 91st Annual Meeting of the Endocrine Society: ENDO 09, Washington, DC, June 10-13, 2009 Vincent B, Gao S, Wang Z: The role of Androgen receptor cofactor p44 in mouse brain aging. University of Texas Health Science Center at Houston Annual Research Day, Houston, TX, November 21, 2008 Vincent B, Gao S, Wang Z: The role of Androgen receptor cofactor p44 in mouse brain aging. Nuclear Receptor Signaling Atlas (NURSA): Androgens, Rotterdam, Netherlands, October 1-3, 2008 Somogyi GT, Vincent B, Salas NA, Boone TB, Smith CP: Activation of Muscarinic(musc) and Nicotinic(nic) receptors evokes ATP and NO release from the Urothelium of rat Urethra. 101th Annual Meeting of the American Urological Association, Atlanta, Georgia, May 20 – 25 2006. J Urol. 2006;175(4 Suppl):1-554 Salas NA, Shirodkar S, Subramanian V, Karpman E, Williams DM, Vincent BH, Lipshultz LI: Microsurgery Elective Course

(640-506) For Medical Students. Academy’s Showcase of Educational Scholarship, Houston, Texas, September 30 2005 Somogyi GT, Vincent B, Salas NA, Boone TB, Smith CP: Activation of Muscarinic(musc) and Nicotinic(nic) receptors evokes ATP and NO release from the Urothelium of rat Urethra. 35th Annual Meeting of the Society of Neuroscience, Washington, DC, November 12-16, 2005

Salas NA, Somogyi GT, Vincent BH, Boone TB, Smith CP: Effect of botulinum toxin A on urethral outlet resistance by measuring changes in leak point pressure. 100th Annual Meeting of the American Urological Association, San Antonio, Texas, May 21 – 26 2005. J Urol 173 (4 Suppl):1-467 Evans JL, Kiss S, Boone TB, Vincent BH, Smith CP, Somogyi GT: Correlation between norepinephrine content and gland weight, age, and PSA in the human prostate. 100th Annual Meeting of the American Urological Association, San Antonio, Texas, May 21 – 26 2005. J Urol 173 (4 Suppl):1-467 Salas NA, Lo KC, Vincent BH, Chuang WW, Shin D, Lipshultz LI: Synthetic human vas model (SilaVasTM): a novel system for the training of physicians to perform a microscopic vasovasostomy. 100th Annual Meeting of the American Urological Association, San Antonio, Texas, May 21 – 26, 2005. J Urol 173 (4 Suppl):1-467 Smith CP, Boone TB, Vincent BH, and Somogyi GT: Urine ATP and adenosine concentration positively correlate with bother score in patients with interstitial cystitis. Joint Annual Meeting of the Society for Urodynamics and Female Urology and the International Society of Pelvic Neuromodulation, Orlando, Florida, February 24 – 27, 2005. Neurourol and Urodryn. 24(2); 163, 2005

Contact this candidate