Assistant Health
Resume:
Seattle, WA *****
linkedin.com/in/paurvi
******.******@*******.***
PAURVI SHINDE
SUMMARY Enthusiastic biomedical researcher with 7+ years of experience in immunology and molecular biology specializing in multi-color flow cytometry, elisa, qPCR, T cell characterization and cell culture assays.
3 years experience in lab inventory management resulting in significant product knowledge and optimization to keep orders within the budget.
Excellent communication and interpersonal skills with reception of travel awards from AAI and ISV congress for presentation of my work and experience organizing several fundraising events.
WORK
EXPERIENCE
Graduate Research Assistant 2012-Present
University of Connecticut Health, Farmington, CT
Designed and performed experiments to study the signaling pathways induced by TLR adjuvants when combined with OX40 co-stimulation that promote CD4 T cell responses.
Identified the novel role of chemokine CXCL9 in accumulation of antigen-specific CD4 T cells in several tissues leading to submission of publication to high impact immunology journal (under revision).
Supervised 2 graduate students over a period of two years helping them transition into their own independent projects.
Presented my work at AAI and ISV congress that helped receive feedback and build connections with a diverse scientific community.
Research Associate 2011-2012
Texas Tech University Health Sciences Center, Abilene, TX
Conducted and coordinated experiments to understand the pathogenesis of Tuberous Sclerosis Complex, a multi-organ tumor suppressor gene syndrome caused by mutations in TSC1 or TSC2 genes.
Successfully analyzed the genes affected by TSC, in TSC1 conditional knockout mice and patient derived angiomyolipoma cell lines, using western blot, ultracentrifugation and coimmunoprecipitation techniques that led to the initial ground work of this project.
As an adjunct instructor, supervised Cisco College students in techniques of PCR, cloning, restriction digestion, ligation, agarose gel electrophoresis, bacterial transfection leading to successful completion of their biotechnology certificate program. Graduate Research Assistant 2009-2010
Texas Tech University Health Sciences Center, Lubbock, TX
Designed and performed experiments to study the role of SMC proteins, myosin light chain
(MLC) and C-kinase activated protein phosphatase-1 inhibitor (CPI-17) in contraction of Page 2
intrapulmonary airways with a goal of understanding the pathogenesis of asthma.
Performed live imaging on mouse lung airways using a phase-contrast microscope, measured changes in calcium signaling using confocal microscope and analyzed the SMC protein phosphorylation using western blot.
This project led to successful completion of thesis and contributed to the publication ‘Ca2+ oscillations, Ca2+ sensitization, and contraction activated by protein kinase C in small airway smooth muscle’.
EDUCATION PhD in Biomedical Sciences, Immunology Concentration, UConn Health, CT 2012-Present M.S. in Biotechnology, Texas Tech University, Lubbock TX, GPA 3.78 2008-2010 B.Tech in Biotechnology, Dr. D.Y. Patil University, India, GPA 3.77 2003-2007 TECHNIQUES
AND
SOFTWARE
Immunology / Molecular Biology
Flow Cytometry
Elisa
DNA/RNA isolation
PCR/qPCR
Cell culture and proliferation assays
Western Blot
Immunohistochemistry
Extensive work of BD LSRII and
MacQuant instruments
Leadership
Public Speaking
Work independently with
minimum supervision
Inventory management
Problem solving and
organization skills
Software
MS PowerPoint/Excel
GraphPad Prism
FloJo 10.2
Adobe Photoshop
Adobe InDesign
Origin Pro
Endnote
PUBLICATIONS Optimal CD4 T cell priming after LPS-based adjuvanticity with CD134 costimulation relies on CXCL9 production. Shinde P, Liu W, Luster A.D, Vella A.T. (Manuscript submitted, under revision).
Ca2+ oscillations, Ca2+ sensitization, and contraction activated by protein kinase C in small airway smooth muscle. Mukherjee S, Trice J, Shinde P, Willis RE, Pressley TA, Perez-Zoghbi JF. J Gen Physiol. 2013 Feb;141(2):165-78. doi: 10.1085/jgp.201210876. AWARDS AND
VOLUNTEER
WORK
Trainee Abstract Award for presentation at AAI. May 2015
Presidential Travel Award for presentation at 16th ISV congress. October, 2016
Organized a team of students at UConn Health to participate in making strides against breast cancer event to be held on 16th Oct, Hartford, CT
Organized a fundraiser at UConn Health for farmers affected by drought in Vidarbha region, India. The event raised a total of 50000 INR. December, 2015
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