Chemical Engineering Development

Cássia Maria Ramaciotti de Andrade

Rockville, MD 20850

1-443-***-****, ******.**********@*****.***

JOB OBJECTIVE

Postdoctoral Fellow looking for an entry level scientist role leading to a career in the development of biopharmaceutical therapies.

EDUCATION

Executive MBA in Administration for Engineers - Institute Mauá of Technology - Brazil (2014)

PhD in Biotechnology - USP - University of São Paulo - Brazil (2013)

Thesis Title: Development of a process for recombinant factor VIII production in bioreactor

Bachelor's Degree in Chemical Engineering - Institute Mauá of Technology - Brazil (2004)

LANGUAGE SKILLS : Fluent English

Intermediate French

Native Portuguese

QUALIFICATIONS SUMMARY

Experience as a Researcher at MedImmune, IPT and at the Laboratory of Environmental Microbiology at USP in new production development processes of products for application in the pharmaceutical, chemical, and oil & gas industries.

Expertise in Biotechnology upstream processes with bacteria, yeast and mammalian cell cultures (anchorage-dependent and suspension), from vial thawing to bioreactor operation in batch, fed-batch or perfusion modes.

Skills in downstream purification processes.

Planning the treatment and disposal of an oil & gas company waste into the environment.

Development of projects with the establishment of partnerships between universities (USP, Mauá Institute of Technology), Technological Centers of Research (IPT - USP, Blood Center), and Industries.

Prospecting, control, and accountability of the budget for the projects and responsible for preparing technical reports.

Professional Background

Dec/14 to present MedImmune/Astrazeneca, Gaithersburg, MD - United States.

Post Doctorate Fellow

- Development of alternatives to current cell culture upstream and purification processes for the production of bispecific antibodies, including the use of new technologies.

- Focus on application of chromatography (HPLC, HPSEC, Affinity, Cation Exchange, Anion Exchange, Mixed Mode) and non chromatography (Precipitation) techniques to improve the aggregate removal capabilities in the downstream purification process.

- Optimization of bioreactor conditions to increase antibody productivity with minimal aggregate formation.

- Transform bacteria, purify plasmid DNA, and transfect CHO host cell line.

- Application of analytical techniques, such as SDS-page gel, western-blot, protein assays, SPR and analytical equipment such as BGA, Horiba, Nova, ViCell, Biacore.

- Active participation in cross-functional teams and multidisciplinary projects with different departments interface.

May/07 to Mar/14 IPT - USP - Institute for Technological Research of the State of São Paulo - Brazil

National Institute of innovation, research, and development.

Researcher (Apr/08 to Mar/14)

- Development of an optimized upstream bioprocess for the production of recombinant factor VIII using human cell line in perfusion mode.

- Implementation of new analytical techniques for evaluation of bioprocesses such as Activate Partial Thromboplastin Time and Chromogenic Factor VIII Activity assay.

- Responsible for preparing technical reports on experimental findings and presenting results in meetings and workshops.

Scholarship Holder - Technological Development (May/07 to Mar/08)

- Carried out a prospective study for identifying, assessing, and prioritizing the use of glycerol (a waste generated in the production processes of Petrobras) as a source of carbon in biotechnological processes aimed at producing products with higher added value.

- Evaluation of the economic feasibility of glycerol by calculating the value of investments and production cost of each product.

- Studied and analyzed the reuse of waste in order to decrease contamination, environmental risks, and thereby avoid their improper disposal.

Sep/05 to Mar/07 Laboratory of Environmental Microbiology - ICB - USP

National Institute of Biomedical Sciences from USP

Scholarship Holder in Technological Development (DTI-CNPq)

- Set up the research project of pre-treatment of the water used in chemical processes for the production process of Petrobras products (oil, gasoline, and petroleum derivatives).

- Development of alternative technologies and methodologies to control chemical and biological fouling of the filter membrane units through the use of biological bioreactors.

Aug/04 to Dec/04 Mauá School of Engineering

Intern

- Analyzed the operating conditions of an absorption column at the pilot plant located at the university and worked with automation projects on it.

AWARDS

"Award from the Alumni Association of the Mauá Institute of Technology (AEXAM) 2004" for the graduate studies paper "Technical Feasibility Study for the automation of an absorption column prototype at the pilot installation of the courses in Chemical and Food Engineering."

CONFERENCES

1. CHUNG, W.K.; ANDRADE, C.; SCHONER, R.; ASPELUND, M.; HUNTER, A. . Improved aggregate removal for bispecific antibodies using a three column, mAb-like platform process. In: RXVII Recovery of Biological Products, 2016, Bermuda.

2. ANDRADE, C. M. R.; AGUIAR, M.A. ; ANDRADE, R.J. ; ALLI, R. P. ; P., Rosane ; LEO, P. ; COVAS, D.T. ; AUGUSTO, E.F.P. . Production of rFVIII using a SKHep stably transfected cells in controled perfusion culture. In: 23rd ESACT Meeting, 2013, Lille. Production of rFVIII using a SKHep stably transfected cells in controled perfusion culture. Lille, 2013. p. 187-187.

3. ANDRADE, C.M.R.; AGUIAR, M.A. ; ANDRADE, R.J. ; COVAS, D.T. ; AUGUSTO, E. F. P. . Influence of microcarrier concentration and ratio cell/microcarrier on a SkHep cell line growth and recombinant factor VIII production. In: 15th European Congress on Biotechnology, 2012, Istambul. Influence of microcarrier concentration and ratio cell/microcarrier on a SkHep cell line growth and recombinant factor VIII production, 2012. v. 1. p. 200-200.

4. ANDRADE, C.M.R.; AGUIAR, M.A. ; ANDRADE, R.J. ; COVAS, D.T. ; AUGUSTO, E. F. P. . Influence of culture conditions on the kinetics and metabolism of suspended Hela cells. In: 22th ESACT Meeting, 2011, Wien. Influence of culture conditions on the kinetics and metabolism of suspended Hela cells, 2011. p. 159-159.

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