State University Assistant

Dr Ketki Sawant

****-* ******* *****, *******-Salem, NC 27106

Mobile: 630-***-****

E-mail: ***********@*****.***

OBJECTIVE

I am actively seeking an entry-level position as a scientist, to enhance my professional growth by providing challenging career while utilizing my existing knowledge and skills. SKILLS

• Molecular biology: Gene and recombinant DNA cloning, genomic PCR, RT-PCR, cloning full length genes by PCR, sub cloning to expression vectors, Gateway cloning, DNA sequencing analysis, site directed mutagenesis, DNA isolation from bacteria, Drosophila whole flies and ovaries and RNA extraction from Drosophila whole flies.

• Cell biology: Drosophila genetics, Microscopy- live cell imaging invivo, immunofluorescence, S2 cell culture.

• Microbiology: Bacterial staining, plating methods (streak, spread, pour, replica), enumeration and identification of bacteria, use of biological safety cabinets, media and buffer preparation, food microbiology, diagnostic biochemical tests for enteric bacteria, use of selective media, serotyping, antibiotic assay (MIC), plaque assay, conjugation.

• Biochemistry: Western-blot analysis, Immunoprecipitation, protein quantization with Lowry and Bradford methods, Immunodetection and cell lysis techniques.

• Computer software: Prism, Microsoft Word, Excel, and Power Point, Adobe Photoshop EMPLOYMENT HISTORY

Research Associate: Dr. Jocelyn McDonald Lab, Division of Biology, Kansas State University (Dec 2015- July 2016)

EDUCATION

• Doctor of Philosophy Degree: Regulatory Biology (Cleveland State University, Ohio) Thesis: Cohesion versus detachment: Roles for Rap1 and its exchange factor, PDZ- GEF in collective cell migration. Graduated: December 2015

• Master of Science: Genetics (Bangalore University, India) Thesis: To study the effect of thyroid hormone drugs on the development of D. melanogaster with the extrapolation to human fetuses. Graduated: June 2009

• Bachelors of Science: Microbiology (Mumbai University, India) Graduated: June 2006

Dr Ketki Sawant

3720-C Ashlawn Court, Winston-Salem, NC 27106

Mobile: 630-***-****

E-mail: ***********@*****.***

CERTIFICATION AND TRAINING

• CITI certification (Online Training): GCP for Clinical Trials with Investigational Drugs and Biologics (ICH Focus),Basic Introduction to Biosafety, Dual Use Research of Concern (DURC),Export Compliance, Responsible Conduct of Research.

• Research Trainee: Student Research trainee at Cleveland Clinic, Cleveland, OHIO

(Oct 2010- Sep 2015)

• Research Intern: Research trainee at Piramal Diagnostic Hospital, Mumbai, India

(Aug 2009- June 2010)

PUBLICATIONS

• Sawant K, Artem Berezovsky, Zong-Heng Wang, Soumya Turaga, Maria Lawas, Erika R. Geisbrecht, Justin D. Lathia, and Jocelyn A. McDonald. Spatially controlled Rap1 activity promotes stability of specific cell-cell adhesions during collective migration. (In Prep)

• Aranjuez G,Burtscher A,Sawant K, Majumder P, McDonald JA. Dynamic myosin activation promotes collective morphology and migration by locally balancing oppositional forces from surrounding tissue. Mol Biol Cell. 2016 Apr 27. Pii: mbc.E15-10-0744. PMID: 27122602

• Geisbrecht ER1, Sawant K, Su Y, Liu ZC, Silver DL, Burtscher A, Wang X, Zhu AJ, McDonald JA. Genetic interaction screens identify a role for hedgehog signaling in Drosophila border cell migration. Dev Dyn. 2013 May;242(5):414- 31. doi: 10.1002/dvdy.23926. Epub 2013 Feb 8.

CONFERENCES

• “Cohesion versus detachment novel role of PDZ-GEF/ Rap1 GTPase in collective cell migration.” At 56th Annual Drosophila conference, Chicago,2015. (Talk)

• “Role of PDZ GEF and Rap1 in border cell migration” at 55th Annual Drosophila conference, San Diego,2014. (poster presentation) REFERENCE

Jocelyn A. McDonald, Ph.D. (Advisor)

Assistant Professor, Division of Biology, College of Arts and Science Kansas State University, Phone: 443-***-**** Email: *******@***.***

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