Maintenance Staff

LILA AVILA, CLSMB

********@*****.***

**** ******** ****** ***•ONTARIO, CA 91761•PHONE 909-***-****

LICENSE Clinical Genetic Molecular Biologist Scientist, California Department of Health and Safety, MTP 001

EXPERIENCE

2015 Mar-2016 Sep Cancer Genetics Inc. (formerly Response GeneticsInc.) Los Angeles, CA

Clinical Genetic Molecular Biologist Scientist in Molecular

• Sequencing and analysis forvarious molecular assays which include EGFR, NRAS, PIK3, HER2

IDH1/2, CKIT, PDGFRA, UGT and BRAF on the ABI 3130XL and 3730XL Genetic

Analyzers.

• Gel electrophoresis.

• Edit Standard Operating Procedures.

• Perform preventative maintenance on equipment.

• QuantifyDNA.

• Set up PCR.

• Supervise work performed by non-licensed technical staff.

• Train staff.

• Perform QA/QC for the laboratory.

• Perform DNA isolations with cobas Roche FDA kit.

• Set up PCR for cobas Roche EGFR and BRAF FDA tests.

2014 Sept-2015 March Sterling Pathology Seal Beach, CA

Clinical Genetic Molecular Biologist Scientist in Molecular and FISH

• R&D start-up for Molecular.

• Write Standard Operating Procedures.

• Perform preventative maintenance on equipment.

• Extract DNA and RNA manually and quantify it.

• Set up RT-PCR.

• Supervise work performed by non-licensed technical staff.

• Train staff.

• Perform QA/QC for the laboratory.

2014 Feb-May Evidia Biosciences, Inc. Diamond Bar, CA

Lead Molecular Technologist in FISH/Molecular Pathology Lab

• R&D start-up for Molecular/FISH.

• Wrote Standard Operating Procedures.

• Performed preventative maintenance on equipment.

• Supervised technical assistant.

• Extracted DNA manually and quantified it.

• Set up PCR and gel checks.

• Performed Sanger sequencing for Factor V, Factor 2, Hereditary Hemochromatosis, MTHFR, APO E

and CYP/VKORC1 testing.

• Last project was BCR/ABL by FISH.

• Performed inventory control/did the ordering for some instrumentation.

• Did initial grossing for tissues for molecular pathology.

2013-2014 Ascend Genomics (formerly PathCentral) Irvine, CA

Lead Genomics Technologist

• Extracted DNA manually and quantified.

• Performed microdissections for FFPE extractions.

• Set up PCR on Viia 7 for JAK2, KRAS and EGFR.

• Assisted with validations for PCR and FISH.

• Set up microarray and loaded on the Agilent.

• Performed FISH hybridization and post hybridization for HER2, Urovysion, BCR/ABL, MDS, CLL,

B-Cell and Multiple Myeloma.

• Performed FISH analysis on Metasystems.

• Prepared FISH reagents.

• Performed manual plasma cell extractions.

• Assisted with revision of Standard Operating Procedures.

• Trained new clients on Tech-only assays.

•Maintained inventory and ordering for the department.

• Maintained all preventative maintenance and QC for the department.

• Coordinated schedules.

• Tracked and logged all samples for monthly metrics.

2008-2014 Neogenomics Irvine, CA

Lead Molecular Technologist in FISH/Molecular Oncology Lab

• Hybridized FISH probes and performed post hybridization stringency washes and counterstain

slides.

• Extracted DNA and RNA from bone marrow, blood and tissue with the Qiagen kits on the Qiacube

automated system.

• Performed plasma cell extractions on the Biomeriux.

• Read FISH slides with fluorescent microscope and analyzed data for BCR/ABL, PML/RARA, HER2,

and many other translocations and break apart probes and bladder cancer on Metasystems and

Bioview.

• Performed DNA and RNA dilutions and quantified on the Nanodrop spectrophotometer.

• Set up RT-PCR.

• Assisted with validations and wrote Standard Operating Procedures.

• Ran JAK2, Exon12, MPL, BCR/ABL, B&T Cell, EGFR, KRAS and BRAF PCR tests and many other

molecular tests.

• Manufactured reagents for home-brewed assays.

• Maintained all equipment in the lab which included Qiagen extraction robot, Rotor-gene

thermalcycler, BioRad thermalcycler and Beckman CEQ genetic analyzer.

• Trained new employees on molecular methods.

• Organized and distributed daily workloads, assisted in scheduling staff, and managed inventory.

2006-2008 Clarient, Inc. Aliso Viejo, CA

Molecular Technologist in FISH/Molecular Oncology Lab

• Received and processed FFPE, blood, cell suspension and urine samples for FISH and PCR.

• Fixed samples to slides and hybridized FISH probes.

• Performed all aspects of FISH testing, from slide preparation, processing, hybridization, post-

hybridization wash, counterstaining, analysis and reporting.

• Analyzed FISH slides for BCR/ABL, PML/RARA, HER2, IgH/CCND1, Urovysion, MDS, CLL, NHL,

MM, and other assays.

• Operated Bioview and Metasystems automated microscope platforms.

• Extracted DNA and RNA dilutions and quantified on Nanodrop spectrophotometer.

•Performed PCR and RT-PCR; assays included BCR/ABL, B&T cell lymphoma, JAK2, and MSI assays

using ABI 3130 and ABI 7900.

• Analyzed patient data and prepared reports for pathologists.

• Tracked and logged samples daily in preparation for the daily operation meetings and monthly

metrics.

• QC’d daily workloads, organized and distributed work assignments.

• Manufactured reagents.

2000-2006 Quest Diagnostics San Juan Capistrano, CA

Molecular Technologist in Molecular Genetics

• Received blood samples and extracted DNA with the automated Qiagen robot and Qiagen kit.

• Set up PCR and ran gel electrophoresis.

• Set up dilutions and quantified DNA with spectrophotometer.

• Performed North2South hybridizations, as well as Southern Blots probed with P-32.

• Used ABI sequencers to assay: Cystic Fibrosis, SNAPshot for Factor V, Sickle Cell Anemia,

Hereditary Hemochromatosis, and many other genetic tests.

• Analyzed patient data.

• Manufactured reagents for all home-brewed assays.

EDUCATION

1994-1999 California State University, San Bernardino San Bernardino, CA

• B.A., Biology

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