ARTI JASROTIA
Seattle, WA
Email: *************@*****.***
Cell: 425-***-****
Authorised to work for any employer in USA
I
am
looking
for
challenging
opportunities,
which
make
the
best
use
of
my
scientific
and
analytical skills and foster my professional and technical abilities, while exposing me to inter- disciplinary biomedical
research
areas.
Work
Experience
Research
Associate
Volunteer
Seattle
Children’s,
Seattle
2016
June- July
Learned and performed standard immunological experiments in a Tcell lab on mice. PMBC isolation from blood, Tcell isolation from Spleen and lymph nodes. Data acquisition by flow cytometer
(LSRII
BD
Fortessa,
HTS
system)
and
data
analysis
by
FlowJo. Research
Associate
Oncology,
University
Hospital
Zurich,
Switzerland 2011- 2015
Thesis:
Role
of
NBS1
in
DNA
double
strand
break
repair
response Advisor:
Dr.
Manuel
Stucki
• Generated
a
novel
complementation
system
to
study
the
effect
of
MDC1
dimerization
in
the DNA
damage
response
and
discovered
that
MDC1
dimerization
plays
in
role
in
cell
survival
• Generated
a
novel
complementation
system
to
perform
detailed
structure/function
analysis of
the
role
of
FHA
and
BRCT
domains
of
NBS1
in
DNA
double
strand
repair
• Discovered
that
the
FHA
and
BRCT
domains
of
NBS1
contribute
in
an
additive
manner
to
the role
of
NBS1
in
DNA
end
resection
• Gathered
experimental
evidence
that
indicated
the
role
of
NBS1
in
DNA
end
resection
may be through a direct interaction between FHA and BRCT domains of NBS1 and phosphorylated
CtIP
• Demonstrated
that
the
FHA
and
BRCT
domains
of
NBS1,
both
contribute
to
the
activation
of G2M
checkpoint
in
an
additive
and
non- redundant
manner
• Developed
a
macro
for
the
analysis
of
large
datasets
obtained
from
fluorescence
microscopy
• Presented
research
work
in
many
national
and
international
conferences/meetings
• Some
part
of
this
work
will
be
published
by
the
end
of
the
year
and
the
remaining
part
will be
published
next
year
Teaching
Assistant
University
of
Zurich,
Switzerland
2012- 2013
Conducted a lab course for a class of 25 undergraduate students of chemistry. Designed experiments, organized study material and lab wares, and checked the experimental results. Designed
questionnaire
and
graded
assignments
that
contributed
to
their
final
exam
grade. Undergraduate
Research
Associate
University
of
Hyderabad,
India
2010- 2011
Tested the role of Hsp82 in DNA repair by in- vitro biochemical assays. Cloned ScHSP82 and ScRAD51 in the expression vectors standardized the expression
of these two genes and
purified the proteins with the help of different tags. Checked the interactions of Hsp82 with different proteins
of
homologous recombination
DNA repair
pathways
by techniques like far- western
and pull- downs.
Education
Ph.D.
in
Cancer
Biology
-
University
of
Zurich,
Switzerland Field:
DNA
repair,
Year:
2011- 2015 Masters
of
technology
-
University
of
Hyderabad,
India Major:
Medical
Biotechnology,
Year:
2009- 2011 Honors,
scholarships
and
awards
• CSIR- Research
Fellowship
2009:
All
India
Rank
- 163
• GATE- 2010:
Percentile
98.50,
All
India
Rank
-
147
• University
of
Hyderabad
Entrance
Exam.
2009:
All
India
Rank
1
• Life
Sciences
Graduate
School
Zurich,
Scholarship
Switzerland
• Travel
award
2015
to
attend
EMBO
conference
in
Athens,
Greece Skills
DNA cloning, PCR, site- directed mutagenesis, confocal microscopy, fluorescent microscopy, western blotting, phospho- protein analysis, siRNA, CRISPR/cas9, Flow- cytometry, generation of mammalian stable cell- lines, tissue- culture, biochemical assays, protein- protein interactions, ELISA, presenting data in a clear and concise manner, scientific writing, data analysis, PBMC isolation,
Tcell
isolation
from
spleen,
scientific
writing,
project
management,
assay
development Certifications:
Design
and
Interpretation
of
Clinical
Trials
by
Johns
Hopkins
University
on
Coursera August
24,
2016
Knowledge
of
clinical
trials
and
GCP,
HIPAA
regulations
and
medical
terminology