Microsoft Office Research
Resume:
MARSHE’ T. EDWARDS, MS, BS
Capitol Heights, MD 20743
******.*******@*****.***
SUMMARY OF QUALIFICATIONS:
Over 4 years of experience in a laboratory setting and 6 years of experience in Histology Research
Laboratory Skills: Immunohistochemistry, Fluorescent Microscope, Confocal Microscope, Alexa Fluor, SDS-PAGE, Western Blot, Gel Electrophoresis, Cell Culture, Animal Handling and Training, iPerfusion Computerized System, Cryostat, Microtome, Dissection, Sigma Plot, MatLab, CO2, Paraffin mount, Liquid Nitrogen and Tissue Specimens, DAKO Autostainer, Aperio Imaging System
Computer Skills: Microsoft Office Suite including Word, Excel, PowerPoint, Outlook and Access
EDUCATION:
MOREHOUSE SCHOOL OF MEDICINE
Master of Science in Biomedical Research 05/2016
BENNETT COLLEGE
Bachelor of Science in Biology and Minor in Chemistry 05/2013
WORK EXPERIENCE
Q2 Solutions 05/2016-Present
Histotechnician
Responsible for the processing and subsequent staining of a wide range of human tissue types
Processing tissues beginning with grossing, embedding and finishing with sectioning and staining tissues
Perform testing on clinical trials samples using immunohistochemistry as well as fluorescent in situ hybridization (FISH), including PDL1, HercepTest, ALK-FISH and HER2 FISH staining
Processing paperwork associated with accessioning and reporting
MOREHOUSE SCHOOL OF MEDICINE
Research Student 08/2013 – 05/2016
Research of Glaucoma and focus in Immunohistochemistry
Sacrificed mice using CO2 and remove eyes of mice
Utilized specific mounts such as Paraffin or Liquid Nitrogen, OTC for mice eyes
Sectioned experiments using Microtome or Cryostat at 10 Microns
Administered Opioid antibodies to tissue specimens
Utilized fluorescent microscope used to measure expression of specific antigens
GEORGIA INSTITUTE OF TECHNOLOGY
Research Student 10/2014 – 05/2016
Glaucoma Research
Removed eyes of mice, following sacrifice using CO2
Cannulated eyes of mice in the anterior chamber of the eye ex vivo
Utilized iPerfusion system computerized system to measure aqueous humor outflow on mouse eye at various pressure steps
Analyzed data using MatLab and Microsoft Excel
INDIANA UNIVERSITY PURDUE UNIVERSITY – INDIANAPOLIS (IUPUI)
Research Intern 06/2010 – 08/2012
Conducted prostate cancer research utilizing Immunohistochemistry
Induced prostate cancer with E-coli by cannulation of mouse genitals ex vivo
Sacrificed the mice with CO2
Dissected out the prostate and embedded in paraffin mounts
Sectioned with Microtome at 10 microns
Received primary Prostate tissue specimens from local hospitals
Applied specific antibodies, LC3 and Caspase 3 to mouse model and primary tissue specimens
Analyzed effects of LC3 and Caspase 3 on mouse model and primary prostate tissue specimens
Performed Western Blot analysis on Prostate Cancer model
Completed homogenization of tissue specimens
Gathered protein and used LC3 and Caspase 3 markers to detect the expression on Prostate Cancer and prostate cancer model
Conducted cell culture of PC3, human prostate cancer cells
Autophagy on PC3 cells by administering chemotherapeutic drug, Docetaxel in various doses
BENNETT COLLEGE AND NATIONAL SCIENCE FOUNDATION (NSF)
Supplemental Instructor 06/2010 – 07/2010
Coordinated and facilitated Summer Academy students with Science classwork, homework and projects
PUBLICATIONS AND PRESENTATIONS
Published a manuscript in the Journal of Urology, March 2015, “Coordinated Induction of Cell Survival Signaling in the Inflamed Microenvironment of the Prostate”
2010 University of Maryland, Baltimore County (UMBC) Undergraduate Research Symposium in the Chemical and Biological Sciences Presentation
2010 North Carolina Alliance to Create Opportunity Through Education OPT ED, NC OPT-ED Conference Presentation
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