NGOC HUYNH

Work

Experience Quality Control Analyst II Jun. 2015 – May 2016

Baxalta Healthcare Corporation, Los Angeles, CA

-Supported lab and validation projects by conducting biological and chemical analyses and prepared technical documentation for Antihemophilic-Factor Method M (AHF-M) test samples and final containers samples collected from thirteen conformance runs.

Coordinated with Manufacturing, Processing to submit in-process samples and final containers samples to Biochemistry and Microbiology Labs

Prepared and completed test requests documentation for in-process samples and final container samples prior to collecting test samples.

Submitted in-process samples to the labs for biological, chemical and microbial analyses.

-Supported product validation by performing biochemical and chemical tests following SOPs for in-process samples and final containers samples.

Determined the amount of impurities and Factor VIII (FVIII) present in conformance samples by performing ELISA tests.

Determined band pattern profiles of FVIII and von-Wilebrand Factor (vWF) by performing Western Immuno Blots for four samples a day.

Determined the activity of FVIII in conformance samples:

oConducted Thrombin-Mediated Activation and Inactivation of FVIII.

oConducted One-Stage Clotting of FVIII using BCS and BCS XP.

oDetermined initial activation rate of Thrombin in conformance samples using Minitab® Software.

-Verified and qualified laboratory equipment for every day use:

Laboratory Analytical Balance

Laboratory Automatic Pipette

pH meter

Spectrometer for colorimetric assays

Made sure equipment validation by reading manufacturing document for Water Bath Thermometer, Dade-Behring Coagulation System (BCS XP)

Collected and recorded data following GMP and GDP.

-Supported in data analyses and final report write-up.

-Supported in maintaining lab instruments validated and calibrated within their due time.

-Provided assistance to project managers by obtaining documents needed to close out deviations and protocol execution binders.

-Reviewed Protocol Execution Binders for GDP errors or missing information.

-Trained on Good Documentation Practices (GDPs), Current Good Manufacturing Practices (cGMPs), SQL*LIMS, ISOtrain and Blue Mountain Regulatory Asset Management (RAM) system, Baxter Product Lifecyle Management (BPLM) User Interface by Instructor-led training (ILT), Gowning and Degowing for classified and unclassified areas, .

-Developed knowledge of Good Documentation Practices (GDPs), current Good Manufacturing Practices (cGMPs), SQLLIMS 4.0, Global LIMS, and Blue Mountain Regulatory Asset Management (RAM) System

University of California, Riverside Sep. 2012 – Jun. 2014

-Determination of single-molecule measurement using magnetic tweezer.

Successfully attached PEG chains onto micrometer-size magnetic beads.

Derivatized glass surface (silanization with 99% efficiency and passivation).

Determined surface hydrophilicity using contact-angle measurement.

Determined micrometer-size beads’ velocities using light microscopy.

-Quantification of metabolite changes in wild-type and stress-treated Arabidopsis.

Extracted trehalose 6-phosphate from other metabolites in plant extract using liquid-liquid extraction and solid phase extraction.

Successfully separated secondary signaling metabolite trehalose 6-phosphate from its isomer sucrose 6-phosphate in Arabidopsis using LC-MS/TOF by Waters Corp.

-Quantification of metabolic changes in different breast cancer cell lines.

Extracted non-polar metabolites from breast cancer samples using liquid-liquid extraction.

Developed a chromatography protocol with short chromatogram window (thirty-six minutes) to separate and quantify fatty acid metabolites using GC-MS by Agilent Technology.

San Jose State University Dec. 2009–Apr. 2012

-Determination of kinetics of novel P450 hybrid enzymes for the catalytic hydroxylation

of fatty acids.

Developed protocols for hydroxylation reaction using hybrid P450 enzymes.

Conducted photochemical experiments.

Derivatized fatty acids utilizing methylation and silylation.

Developed chromatography methods in GC-MS to separate hydroxyl fatty acids.

Identified and quantified product mixture using GC-MS.

Determined Michaelis-Menten kinetics parameter.

Skills ELISA

Western Immuno Blot

BCS, BCS XP

Surface silanization and passivation, magnetic bead PEGylation

Contact-angle measurement

Oxygen plasma treatment

Optical microscopy

Reflection interference contrast microscopy (RICM)

GC-FID (Agilent Technology)

GC-MS (Agilent Technology)

LC-MS/TOF (Waters)

UV spectrometry.

Molecular biology techniques, including PCR, DNA extraction, ligation, transformation.

Microbiology techniques, including plate streaking, cell culture

Lyophilization equipment, autoclave

Liquid-liquid extraction, solid phase extraction, buffer preparation

R statistical analysis

Minitab®

LabView

LIMS/LIMSLINK

cGMP, GDP

IsoTrain

Gowning in classified and unclassified area

Blue Mountain Regulatory Asset Management (RAM) System

Poster Presentations

“A new hybrid enzyme for the selective hydroxylation of substrate unactivated C-H bond using light and water” P. Ngoc, M. E. Cooper, N. Huynh, M. Au, L. Cheruzel

“Hybrid P450 BM3 enzymes with light-activated artificial electron donor” Ngoc-Han Tran, Ngoc Huynh, Thuba Bui, Lionel Cheuzel

“Engineered P450 BM3 enzymes towards specific C-H bond oxidation using light activation” N. H. Tran, T. Bui, N. Huynh, L. Cheruzel

“Light initiated hydroxylation of substrate C-H bonds using hybrid P450 enzymes” Ngoc Huynh, Ngoc-Han Tran, Thuba Bui, Angelina Nguyen, Yen Nguyen, Jeremiah Heredia, Garrett Chavez, Lionel Cheruzel

Oral Presentation

“A new approach to hydroxylation of fatty acids using hybrid P450 BM3 enzyme”

Publications

Tran, N. H.; Huynh, N.; Bui, T.; Nguyen, Y. and Cheruzel, L. Light –Initiated Hydroxylation of Lauric Acid Using Hybrid P450 BM3 Enzymes Chem. Comm., 2011, 47, 119**-*****.

N.H. Tran, N. Huynh, G. Chavez, A. Nguyen, S. Dwaraknath, T.A Nguyen, M. Nguyen, L.E. Cheruzel A series of hybrid P450 BM3 enzymes with different catalytic activity in the light-initiated hydroxylation of lauric acid. J. Inorg. Biochem. 2012, 115, 50-57.

Strengths

Responsible, reliable and collaborative with other team members.

Quick learner, excellent time management and organization skills.

Extracurricular Activities

Active Member of World Mission Society Church of God and Nov. 2014 – present

participated in displaying love to community

Painting park benches and bleachers at Davis Park, Colton, CA Nov. 2015

Cleaning the Colton City Bike path and appeared in Colton City paper news Dec. 2015

3033rd Worldwide Environmental Clean-Up Campaign: Feb. 2016

-Painting picnic tables and benches at Colton Plunge Park and appeared in Colton City news.

602nd Worldwide Blood Drive to Give Life through the Love of the Passover Apr. 2016

and appeared in Colton City news.

Cleaning Up Walk Path at CSU San Bernardino and appeared in San Bernardino Jun. 2016

County newspaper.

Giving Love to Elderly in Redlands Nursing Homes on Father’s Day Jun. 2016

and appeared in Colton City news

Science Olympiad Coach for Bungee Drop Event at RSA Nov. 2014 – May 2015

Assisted four middle school students per group to develop bungee drop contest apparatus.

Provided strategies and creative thinking for students to construct testing apparatus from scratch.

Volunteer – Information Desk Assistant Jun. 2010–Mar. 2011

Santa Clara Valley Medical Health Center

Provided visitors with general information and direction to various departments.

Assisted visitors and families to their patients’ location.

Education

M.S. in Chemistry Aug. 2014

University of California, Riverside

B.S. in Chemistry with concentration in Biochemistry Dec. 2010

San Jose State University, San Jose, CA

A.S. in Biological Sciences Aug. 2008

De Anza College, Cupertino, CA

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