SANJIV GUPTA

**** ******* *****, *****, ********** 95616

530-***-**** home

530-***-**** cell acvsh4@r.postjobfree.com

Developed specialized cellular, biochemical, Immunological and molecular studies of various proteins in isolated cells and animal tissues. Exhibits strong communication and organizational skills and an ability to work effectively in coordinating research efforts in a team environment and across research groups.

Education

CENTRAL DRUG RESEARCH INSTITUTE, Lucknow, India

Ph.D. in Biochemistry

G.B. PANT UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, Pantnagar, India

Master of Science in Biochemistry with minor in Microbiology

MEERUT UNIVERSITY, Meerut, India

Bachelor of Science in Chemistry, Biology

OTHER

Certified Training in Radiation Safety, Chemical lab Safety, animal care and handling

Workshop on sophisticated instruments: Successfully participated in the theory and practical of 1. NMR Spectroscopy 2. Mass Spectrometry 3. HPLC 4. Scintillation Spectrometry 5. Electron Microscopy 6. Computerized Information System

Annual Workshop in Cell and Molecular Techniques

Skills

Proteins and Assays:

Characterization and purification of recombinant proteins and their modifications identification by amino acid sequencing,

Characterization and purification of enzymes and isozymes, development of assays to access their purity

Immuno-precipitation, collection and separation of blood and plasma proteins, cell counts, hematocrit and ESR, isolation and purification of serum proteins, coagulation and prothrombin time,

Antibody production and purification, isoelectric-focusing, electrophoresis (1D and 2D),

ELISA, Standardization of immuno-diagnostic ELISA assays for cardiomyocytes.

Post-translational protein modifications, identification by western blot, amino acid sequencing, TLC, column chromatography, HPLC and GC-MS.

Enzyme assays and immuno-localization. Radio-ligand receptor binding analysis.

Biochemical techniques related to quantifying receptors and receptor function.

Lab Experimentations

I have isolated and purified heat shock protein 60 from adult rat cardio-myocytes by following protein biochemistry techniques as collagenase perfusion of heart, isolating and culturing of myocytes, isolating cell organelles by differential centrifugation and performing electrophoresis, western blotting to purify proteins.

I have studied the mechanism of toxicity of Diquat, a pesticide and could be present in drinking water in minute amounts it is a redox cycling compound and cause damage to the body tissues by the generation of reactive oxygen species.

I have worked on Glutathione transferases, which are multifunctional enzymes providing protection to cells from toxic substances including carcinogens through several different activities. These enzymes could either catalytically or non- catalytically bind to toxic metabolites in the body rendering them inactive.

I have done extensive research to study the role of anti-oxidants in the prevention of breast cancer. I have studied the role of resveratrol, a polyphenol antioxidant found in grapes, in human brain, neuroblastoma, SH-SY5Y cell lines (ATCC-CRL-2266) subjected to oxidative stress.

Lab Equipments and Tools

Extensive experience of Gel Electrophoresis, Western Blotting, TLC, Column Chromatography,

Extensive experience of Gradient and Ultra-Centrifugation, Spectrophotometer

Use of Biosafety cabinets and incubators for Cell Culturing

Expertise in preparation and Sterilization of Cell Culture media by autoclaving

Working knowledge of Thermocycler and flow cytometer

Isoelectric Focusing

Working knowledge of HPLC and GCMS and amino acid sequencer

Protocol

I have experience in planning and executing projects, method development

I have used several ELISA and PCR kits and followed protocols provided in kits

I have prepared my own or modified the existing protocols while planning research experiments.

Statistical Data or Documentations

Analyzing the data and statistical analysis.

Knowledge of computer programs as MS Word, Excel, PowerPoint, Adobe Photo Shop and Sigma Plot.

Reviewing the current literature including Medline and Pubmed,

Writing scientific literature, reviews and publications

Troubleshooting and interpreting results, writing scientific reviews, publications and grant proposals

Keeping records and inventory of chemicals and controlled substances ordering supplies, keeping track of funding.

Technical Proficiencies

Protein Biochemistry/Immunology

Microbiology

Culturing and handling of microorganisms, sterile lab techniques, preparing sterile media, plates and cell culture, primary and secondary cell culture

Fluorescent microscopy, laser scanning confocal microscopy, Software proficiencies include scientific applications such as Sigmaplot analysis and general Microsoft Office software.

Molecular and Biochemical Techniques

Extraction and purification of DNA and RNA, cDNA synthesis, PCR, RT-PCR, Extraction of plant hormones, aldehydes, and volatile metabolites, and their quantification by gas chromatography and mass spectrometry, primer design optimization for pcr and qpcr.

Management Techniques

Reviewing current literature including Medline and Pubmed, writing scientific literature, first authored several peer-reviewed publications. Excellence in Microsoft Office. Adobe Photo Shop and Sigma Stat software.

Establishing and managing lab, planning and executing projects, method development, supervising and guiding junior scientists and students, ordering supplies, keeping track of funding, writing animal protocols, maintaining and repairing lab instruments, keeping lab records, preparing lab for inspection, troubleshooting and interpreting results, writing scientific reviews, publications and grant proposals, establishing lab, keeping record and inventory of chemicals and controlled substance s and disposal of Biohazard materials. Maintaining lab safety and training records.

Academic Honors and Fellowships

Graduate Teaching and Research Fellowship, Master’s Program

Council of Industrial and Scientific Research Fellowship

Teaching Experience

Taught Biochemistry Courses to undergraduates during MS Program

Book Reviewed

Enzyme Technology in Beam House Practice by R. Puvankrishanan and Susil C. Dhar, NICLAI Publication, Central Leather Research Institute, Madras, pp, (iii-+175). This is a treatise on beam-house practice on the use of enzymes in the leather industry and technology.

Citizenship

US Citizen

Professional Experience

Adjunct Instructor (2015- present) Department of Biology, WCC, Woodland, CA 95616

Researcher (2011-2013), Department of Plant Sciences, University Of California, Davis, CA 95616

Junior Specialist (2008-2011), Department of Nutrition, University of California, Davis, CA

Selected PEER- REVIEWED Publications:

1. Gupta, S., Srivastava, D.K., Varma, V. and Shukla, O.P.: Drug metabolizing enzymes and cytochromes of Acanthamoeba culbertsoni (Singh and Das,1970). Indian Journal of Parasitology. 1983; 7(2): 175-177.

2. Gupta, S., Kaul, S.M., Imam, S.A. and Shukla,O.P.: Polyamines in Acanthamoeba culbertsoni and

Entamoeba histolytica. Indian Journal of Parasitology. 1984; 8(2): 223-226.

3. Gupta, S., Srivastava, D.K., Saxena, J.K., Tandon, J.S. and Shukla, O.P. Differentiation of Acanthamoeba culbertsoni: cyclic nucleotides and encystation. Indian Journal of Experimental Biology. 1986; 24: 235-237

4. Gupta, S., Kishore, P. and Shukla, O.P.: Effect of phenothiazine compounds and sinefungin on growth of Acanthamoeba culbertsoni (Singh and Das,1970). Indian Journal of Parasitology. 10(2): 177-179, 1986.

5. Gupta, S., Saxena, J.K., Kaul, S.M., Imam, S.A. and Shukla, O.P.: Protein Kinases of Acanthamoeba

culbertsoni and Entamoeba histolytica. Indian Journal of Parasitology. 10(1): 27-32, 1986.

6. Gupta, S., Srivastava, D.K. and Shukla, O.P.: Inhibition of polyamine metabolism induces amoebic

encystation. Current Science 55(15): 732-734, 1986.

7. Gupta, S., Varma, V., Bose, S.K. and Shukla, O.P.: Cytochrome P450 and drug metabolising enzymes of Acanthamoeba culbertsoni. Medical Science Research. 1987; 15: 1379-1380.

8. Gupta, S., Shukla, O.P. and Walter, R.D.: Putrescine activated S-adenosylmethionene decarboxylase from Acanthamoeba culbertosoni. Molecular and Biochemical Parasitology. 1987; 23: 247-252.

9. Gupta, S., Kishore, P. and Shukla, O.P.: Polyamine metabolism as a target of growth inhibition of

Acanthamoeba culbertsoni. American Journal of Tropical Medicine and Hygiene. 1987; 37(3): 550-555.

10. Gupta, S., Ahmad, H., Haque, A.K. and Awasthi, Y.C.: Glutathione S-transferase of human lung.

Evidence that two class subunits are expressed differentially in human population. Clinical

Chemistry and Enzymology Communications. 1990;3: 115-124.

11. Gupta, S., Rogers, L.K. and Smith, C.V.: Biliary excretion of lysosomal enzymes, iron, and oxidized

protein in fischer-344 and sprague-dawley rats and the effects of diquat and acetaminophen. Toxicology

12. Gupta, S., Rogers, L.K., Taylor, S.K. and Smith, C.V.: Inhibition of carbamyl synthetase-I and glutamine synthetase by hepatotoxic doses of acetaminophen in mice. Toxicology and Applied Pharmacology. 1997; 146:317-327.

13. Gupta, S., Kleiner, H., Rogers, L.K., Lau, S.S. and Smith, C.V.: Redox stress and hepatic DNA

fragmentation induced by diquat in vivo are not accompanied by incerased 8-hydroxydeoxyguanosine

contents. Redox Report. 1997; 3(1): 31-39.

14. Gupta, S., Husser, R.C.,Geske, R.S., Welty, S.E. and Smith, C.V.: Sex differences in diquat-induced

hepatic necrosis and DNA fragmentation in Fischer-344 rats. Toxicol. Sci.2000; 54(1):203-211.

15. Gupta, S and Knowlton, A.A. Cytosolic heat shock protein 60, hypoxia, and apoptosis. Circulation. 2002; 106: 2727-2733.

16. Gupta, S and Knowlton, A.A. HSP60, Bax, Apoptosis and the Heart. J. Cell. Mol. Med. 2005; 9 (1):51-58

17.Gupta, S and Knowlton, A.A. HSP60 trafficking in adult cardiac myocytes: role of the exosomal pathway. Am. J. Physiol. Heart Circ. Physiol. 2007; 292(6):H3052-H3056

Selected Conference Presentations:

Effects of acetaminophen and diquat on lysosomal exocytosis in rats in Meeting of Society of Experimental Biology in New Orleans, Louisiana

Xenobiotic and drug metabolizing enzymes of A.culbertsoni in Meeting of society of Biological Chemists in Pune (India)

Expression of Glutathione transferase in mouse tissues in meeting of Society of Experimental Biology in New Orleans, Louisiana

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