Manager Technician

CURRICULUM VITAE

Name: OMAR ALVARADO, MA, BS

Ph: 626-***-**** email: acvniy@r.postjobfree.com

Education: California State University Fullerton

August 1997-June 1999

Bachelor of Science in Biology

University of California Los Angeles

1995-1997

Master of Arts in Psychology

University of California Los Angeles

1991-1995

Bachelor of Arts in Psychology

Employment History:

Assistant Laboratory Manager, May 2013-August 2015

Department of Research and Biotechnology, supervisor: Dr. Henriksen

Western University of Health Sciences, Pomona, CA

Research Laboratory Technician, January 2007-March 2013

College of Veterinary Medicine, supervisor: Dr. Rutllant

Western University of Health Sciences, Pomona, CA

Microbiology Lab Technician, July 2006-Jan 2007

Kelly Scientific

3602 Inland Empire Blvd, Suite B-100

Ontario, CA 91764

Quality Lab Technician, Apr 2006-July 2006

Musculoskeletal Transplant Foundation

3535 Hyland Ave.

Costa Mesa, CA 92626

Laboratory Technician, March 2005-March 2006

Lab Pros (Guidant Corporation) R&D Company

445 East Gate Mall, Suite 200

San Diego, Ca 92121

Laboratory Aide III, October 2001-January 2005

California Pacific Medical Center

2333 Buchanan St.

San Francisco, CA 94115

Laboratory Technician, January-October 2001

National Genetics Institute

2440 S Sepulveda Blvd # 235

Los Angeles, CA 90064

Professional Qualifications:

A.Laboratory Skills:

Strong cell and molecular biology techniques

Expertise in mammalian cell culture (uninfected and infected lines); experienced with primary cells

Extensive experience in flow cytometry, fluorescence microscopy, ELISA

Experience working in a quality assurance/control lab and related knowledge of GMPs and cGMPs

Extensive experience with designing/planning experiments, developing protocols and consulting on a wide-range of projects

Competent in cell proliferation and cytotoxicity assays

Proficiency in Western blotting; experienced in hybridization and Northern and Southern blotting

Skilled in immunostaining, as well as several other staining techniques

Competent in immunoprecipitation and lysate preparation

Highly proficient in media and buffer preparation

Rat handling, feeding and euthanization

Limited knowledge of breeding colony management

Histological techniques (slide preparation from tissue and staining)

Experienced in working with BSL2 agents (e.g. Hepatitis B, FIV, HIV)

Protein purification, DNA and RNA purification

Quantitative PCR, electrophoresis, limited qPCR

Spectrophotometry

Handling and preparation of blood, raw tissue, cell culture and semen samples

Identification, culture, isolation and plating of bacterial strains

Bacterial transformation and cloning

Extensive knowledge of vivarium environmental monitoring

B.Managerial Skills:

Aiding in the development and implementation of university-wide laboratory safety and training programs according to GLPs and OSHA standards as well as SOP development for training purposes

Maintenance, repair and calibration of various types of laboratory equipment (sterilization and water purification systems, microscopes, spectrophotometers, flow cytometer, laminar flow hoods and pumps, etc.)

Training of students, staff and faculty on equipment use and GLPs

Coordinated with EH&S department to ensure availability of spill clean-up kits as well as proper disposal and regular pickup of biohazardous wastes, animal carcasses, chemicals, sharps, broken glassware, etc.

Supervision of laboratory staff to ensure compliance with GLPs and OSHA standards as well as proper waste disposal

Laboratory ordering; inventory and SDS management of reagents and supplies

Interfacing with other departments (i.e. Facilities, Purchasing and Environmental Health and Safety) to prevent and/or resolve issues efficiently

Addressing employee concerns and resolving issues quickly to increase productivity

Planning/organizing daily, weekly and monthly laboratory operations to either solve issues or prevent them (e.g. monitoring equipment functionality, lab safety inspections, monitoring of routinely used supplies, environmental monitoring, etc.)

Knowledge of environmental conditions necessary for vivariums according to AAALAC standards for individual animals

Assigning specific duties/responsibilities to individual lab managers/technicians to increase efficiency/productivity and lower maintenance costs

Implementing, planning and facilitating monthly meetings among individual labs to increase communication and work flow

Analyzing daily laboratory operations and communicating with staff to determine where improvements can be made

Providing tours to companies, visitors and guests

C.Other Skills:

Bilingual in English and Spanish

Flexibility, adaptability, ability to work and learn independently as well as part of a team

Self-motivated, innovative thinker

Results-oriented, analytical problem-solver; excellent troubleshooting skills

Excellent social, motivational and communication skills

Preparation of posters for presentations and manuscripts for publication

D.Computer Skills:

Efficient in use of Microsoft Office programs

Various data/statistical analysis programs, including Prism, SPSS, MiniTab

Expertise in use of FlowJo software for flow cytometry; training of colleagues

Image J and Nikon inverted fluorescent microscope software

E.Certification, Continuing Education & Training:

Certificate of Completion of Comprehensive Flow Cytometry Course from FloCyte Associates, 2009.

Teaching Activities:

Guest Speaker, College of Veterinary Medicine, 2012

Western University of Health Sciences

Scientific Publications:

Testa MP, Alvarado O, Wournell A, Lee J, Guilford FT, Henriksen SH, Phillips TR. Screening assay for oxidative stress in a feline astrocyte cell line, g355-5. J Vis Exp. 2011 Jul 13;(53). pii: 2841. doi: 10.3791/2841.

Guerra C, Johal K, Morris D, LittleJohn E, Moreno S, Alvarado O, Gray D, Tanzil M, Pearce D, Venketaraman V. Control of Mycobacterium tuberculosis growth by activated natural killer cells. Clin Exp Immunol. 2012 Apr; 168(1):142-52.

Pham AN, Blower PE, Alvarado O, Ravula R, Gout PW, Huang Y. Pharmacogenomic approach reveals a role for the x(c)- cystine/glutamate antiporter in growth and celastrol resistance of glioma cell lines. J Pharmacol Exp Ther. 2010 Mar;332(3):949-58. Epub 2009 Dec 9.

Scientific Posters:

Guerra C, Johal K, Morris D, Moreno S, Alvarado O, Venketaraman V. Growth Inhibition of Mycobacterium tuberculosis using natural killer cells isolated from healthy and HIV infected subjects. Presented at Western University of Health Sciences, Pomona, CA, 2011.

Porterfield L, Alvarado O, Rutllant J. The use of canine testicles as a model to retrieve viable epididymal spermatozoa from canine endangered species. Presented at Morris Animal Foundation Wildlife Symposium, New Orleans, LA, 2011.

Rodriguez S, Alvarado O, Phillips T, Phillips M. Comparing the effects of GSH and liposomal GSH on oxidative stress in uninfected vs FIV infected astrocytes. Presented at the National Veterinary Scholars Symposium, Athens, GA, 2010.

Gonzalez B, Alvarado O, Guerra C, Johal K, Cundari A, Chan J, Venketaraman V. Effects of glutathione on dendritic cell response to Mycobacterium tuberculosis infection. Presented at California State University Los Angeles, Los Angeles, CA, 2010.

Johal K, Guerra C, Gonzales B, Alvarado O, Vishwanath Venketaraman, V. Components of innate and adaptive immunity in cooperation with increased glutathione levels against Mycobacterium tuberculosis infection. Presented at California State Polytechnic University, Pomona, CA, 2010.

Goodwin E, Alvarado O, Phillips T, Testa M. Inhibition of Caspase 8 and its effect on FIV+ astrocytes in vitro. Presented at University of Zagreb, Croatia, 2009.

Rutllant J, Alvarado O, Fonda, ES. Effects of trehalose on the osmotic stress limits of boar sperm. Presented at the Society for the Study of Reproduction, Hawaii, 2009.

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