Dipti Budhdev
Atherton, CA 94027
************@*****.***
Education
B.S. in Biotechnology University of California, Davis, CA June 2015 A.S. in Biological Sciences Cañada College, Redwood City, CA June 2013 OPT up to September 19, 2017
Skills
• Regulatory – National and International, FDA/cGMP, USP/EP
• Investigations and TrackWise collaboration
• eLIMS/ LabWare LIMS for reagent receipt and product testing
• 5S/ 6 Sigma Lean lab skills
Immunology/Molecular Biology/Biochemistry
• cDNA library preparation
• DNA/RNA isolation, quantification and analyses, PCR, RT-PCR, use of Roche LightCycler ® 480
• Handling mammalian cell cultures
• Electrophoresis (agarose, SDS-PAGE), Western and Southern blotting
• Staining – Gram and Negative – to identify bacteria, Microscopy – Brightfield
• Preparation of pure bacterial cultures
Computer/Bioinformatics
• PC and Mac. Microsoft Office: Word, Power Point, Excel
• Linux and Windows Operating systems
• Databases: NCBI, JSTOR, Google Scholar, Patents
Work/ Research Experience
QC Analyst II – QCBAT (QC BioAnalytical Testing)/ Commercial April – June 2016 Genentech, Inc.
• Worked in a GMP environment
• 5S auditor – planning, inventory and Kanban fundamentals
• FDA mock audit participation, knowledge of flow of audits
• UV-Vis spectrophotometer system suitability and cuvette checks – gravimetric and volumetric
• Pipette maintenance – operator/ verifier for sending pipettes out for calibration
• Centrifuge/ incubator/ plate washer maintenance
• Performer/ reviewer – balance checks, temperature alarm checks
• Reagent making/ receipt using reagent receipes database
• Verifier for reagent receipt for label printing
Laboratory Technician – Raw Materials July 2015 – Feb 2016 Janssen Pharmaceuticals, Pharmaceutical Companies of Johnson and Johnson
• Sample receipt - scheduled and non-scheduled drugs - using eLIMS database
• Analytical Requests log-in/testing
• Tested raw materials for transdermal products of Scop, Nicoderm and Catapres
• Trained new Technicians for eLIMS sample receipt
• Solution prep for Raw Materials and Solids groups
• Laboratory maintenance – stocking lab supplies
• Gained national and international collaborative skills Undergraduate Researcher, UC Davis, Davis, CA Nov 2013 - March 2015 Laboratory of Prof. Christoph Vogel, Department of Environmental Toxicology
• Lab research focus: Understanding molecular mechanisms for the function of xenobiotics and inflammatory signaling to immune responses when exposed to environmental toxins. Deployed RNA extraction and RT-PCR methods to test gene expression of tissues exposed to toxins.
• Team work in research projects with Prof. Vogel and other lab members
• Trained exchange students from Germany and Mexico on PCR data analyses Chemistry Laboratory Assistant Jan 2013 - May 2013 Cañada College, Supervisor: Roslind Young, Department of Chemistry
• Maintained laboratory inventory and assisted two chemistry instructors for making unknowns for chemistry lab courses along with independent experimental quality control testing.
• Gained collaborative skills
Undergraduate Researcher
Cañada College, Laboratory of Dr. Carol Rhodes, Department of Biology Spring 2012
• Project: Investigated Wolbachia bacterium infection in insects and spiders. Extracted DNA from each organism, performed PCR and analyzed agarose gel electrophoresis results to look for the infected organism.
• Gained experience with developing a research proposal and execution Honors/Awards for Academic Excellance
• Luther D. and Marie M. Davis Scholarship Award UC Davis 2013-2014
• Iliff Scholarship Award Cañada College 2013
Presentation
Undergraduate Research Conference UC Davis May 2015 P.I. : Prof. Christoph Vogel
Poster presentation from undergraduate research project: Altered Expression of Inflammatory Cytokines in response to LPS and TCDD in wild type (WT) and AhRR Transgenic C57BL/6 Mice