Research Assistant

Jing Han

*** ******** *****, ***** **********, NJ 07080 ● 617-***-**** ● *******@*****.***

SUMMARY

Three years of biological lab experience, including all aspects of DNA sequencing work: prepared human DNA using amplification techniques, carried out DNA sequencing and analyzed the results with genetic tools. Two years of customer service experience in forward-facing roles.

LABORATORY SKILLS

Molecular Biology: DNA sequencing, PCR, Western & Southern Blot

Bioinformatics: Sequencer, TaqMan, Perl scripting, data analysis using Excel and mySQL

Microbiology: DNA/RNA/protein extraction, Bacteria & archaea cell culture, microarray, biofilm assay analysis

Histology: Staining with classical & fluorescent stains, light and fluorescent microscopy, image processing

RESEARCH EXPERIENCE

Graduate Research Assistant

2007 – 2010

University of Maryland, Baltimore

Baltimore, MD

Genetics research of diabetes

• Amplified and sequenced Omentin promoter from diabetes patients

• Screened primers and optimized PCR conditions for Omentin Single Nucleotide Polymorphism (SNP) analysis

Genetics analysis of stroke

• Completed a database containing all the SNPs related to stroke genetics

• Calculated quality control parameters within this database

Biofilm research of H. la

• Optimized culture conditions for biofilm induction in haloarchaea H. lacusprofundi

• Developed a quantitative method to measure the amount of biofilm formed in different conditions

• Completed a bioinformatics project on prokaryotic and eukaryotic protein clusters by writing Perl script and utilizing MySQL database

Genetics analysis of E. coli biofilm

• Analyzed genetics factors associated with virulent biofilm formation in atypical enteroaggregative E. coli (EAEC)

• Performed random transposon mutagenesis to generate multiple strains of EAEC 101-1

• Applied a biofilm assay to examine the effect of these mutations

Study of zinc channel structure

• Prepared PC-3 cell lysates and determined protein concentration by BCA assay

• Transient transferred PC-3 cells with zinc channel expressing plasmid and studied zinc channel structure in cell

OTHER EXPERIENCE Librarian Assistant Secaucus Public Library

• Worked at the front desk serving patrons

2014 – 2015

Secaucus, NJ

• Developed new clients from walk-ins and telephone calls from students looking to study abroad

• Communicated regularly with clients by phone and e-mail

Secretary of the Chairman

2011

Senkai Investment

Guangdong, China

EDUCATION

Master of Science, Biomedical Sciences

2010

University of Maryland, Baltimore

Baltimore, MD

Bachelor of Science, Oral Medicine

2007

Shandong University

Shandong, China

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