Laboratory Technician, 5+ years experience
Resume:
AARON BRUNO
San Jose, CA
*********@*****.***
EDUCATION:
June 2004 University of Illinois at Chicago
December 2007 BS Biology
August 2002- University of Connecticut
May 2004 Storrs, CT
Sophomore Standing
Major: B.S. in Biology
EMPLOYMENT:
May 2013- Medimmune, Inc.
July 2016 Mountain View, CA
Laboratory Technician,
Responsible for monitoring stock levels and
Prepared routine lab reagents, buffers, tissue culture materials and any other solutions, requested by end users, via Lab Services Media Request SharePoint
Performed Aseptic cell culture techniques required for monitoring stock levels and subsequent aliquot preps of Research group's specific reagents
Performed autoclaving/sterilization of dry and liquid materials as requested by end users
Monitored, replaced and ordered gases for the site. Ordered, monitored levels and replaced LN2 for cryo freezers
Ordered general lab supplies and chemicals for lab groups
Restocked laboratories with consumables
Collected pipettes due for calibration and sending out for calibration on a monthly basis
Collected dirty glassware from labs, wash and sterilize and redistribute to satellite storage areas.
Received, processed and catalogued samples from various worldwide locations
Performed troubleshooting on lab techniques and processes, including maxi/midi preps and ELISA assays
August 2012- Eurofins STA
October 2012 Gilroy, CA
Lab technician, Plant Health Services
Performed tasks in a client-based laboratory
Performed and documented ELISA data for 15 viruses
Performed RT-PCR RNA to cDNA
Ran and documented Gel Electrophoresis data
Maintained ELISA lab supplies, documentation and workspace
Performed specimen collection from plant samples
Used Excel to generate data
October 2011- Life Technologies, Inc.
December 2011 Foster City, CA
Research Technician; Validation and Verification Team
Performed tasks independently and as a member of the team in an industry setting
Organized and followed protocols and schedules and deadlines
Set up and ran plates for the 3130, 3500 and 310 ABI Capillary Electrophoresis systems
Performed Trouble-shooting and independently maintenance on ABI equipment March 2009- University of Illinois Chicago
March 2010 Chicago, IL
Research Assistant Department of Pharmacology
Developed protocols for mouse monocyte extraction from lung
In charge of mouse genotyping with gel electrophoresis
Performed transformation and purification of plasmid DNA
Placed in charge of purchasing lab supplies and reagents.
Placed inn charge of all paperwork in the lab pertaining to orders
Performed Fragment analysis using Genemapper 4.1
Archived and organized plasmid DNA samples
September 2006- University of Illinois Chicago
March 2010 Chicago, IL
Laboratory Assistant DNA Services
Research Resources Center
In charge of project to extract RNA from whole blood samples
Involved in Helicobacter project, extracting and purifying Helicobacter DNA from human and mouse stool samples
Performed extraction of DNA from feces
Performed PCR on Nucleic Acid
Experience in DNA Sequencing of customer samples
Experience in growing, plating and midi prepping Ecoli
Initiate the organization of lab plasmid stocks
Made and ran ETBR gels
Performed PCR cleanup
Performed Plasmid DNA preps on bacterial cultures
Maintained buffer stocks
Worked with the ABI 3500XL and 3730 capillary electrophoresis machines including maintenance
Worked alongside customers and clients to complete tasks and projects June 2004- University of Illinois at Chicago
August 2006 Chicago, IL
Laboratory Assistant for the Department of
Hematology/Oncology
Grew, fed and split MCF70 cells
Helped customers with running Flow Cytometry
Performed maintenance on FACS sorter
HONORS AND Maryland Schools Certificate of Merit
AWARDS Advanced Placement Scholarship
PUBLICATIONS
Nunez R, Garay N, Bruno A, Villafane C, Bruno E, Filgueira L. Functional and structural characterization of two populations of human monocyte-derived dendritic cells.
Exp Mol Pathol. 2004 Oct;77(2):104-15.
PMID: 15351233 [PubMed - indexed for MEDLINE]
Nunez R, Garay N, Villafane C, Bruno A, Lindgren V., Description of a flow cytometry approach based on SYBR-14 staining for the assessment of DNA content, cell cycle analysis, and sorting of living normal and neoplastic cells. Exp Mol Pathol. 2004 Feb;76(1):29-36.
PMID: 14738866 [PubMed - indexed for MEDLINE]
REFERENCES:
1.Mingjiang Xu MD, PhD., *********.**@****.*** 212-***-**** 2.Annette Meyer *******.******@*****.*** 312-***-**** 3.Ryon Bordoni ****.*******@********.***
4.Lachan Band **********@*****.***
5.Zaida Morales **********@***.***
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