CURRICULUM VITAE

Brad Duncan Low, M.S., M(ASCP)CM

*** ***** ***** ******

Tyler, TX 75702

903-***-**** (Cell); 903-***-**** (Work)

acv36p@r.postjobfree.com

JOB OBJECTIVE

I am seeking this position to utilize the skills I have acquired from certification in Clinical Microbiology. I wish to expand my career and become an effective team member in employing my education, training, professional experience and problem solving abilities. I have completed coursework for certification in Clinical Chemistry and I will able to add that skill set to this position.

BOARD CERTIFICATION

ASCP; Technologist in Microbiology (M) certificate #6168, June 2016

EDUCATION

Clinical Chemistry Certificate, The University of Texas Medical Branch, Galveston, TX, anticipated date August 2016

Clinical Microbiology Certificate, The University of Texas Medical Branch, Galveston, TX, December 2015

M.S., Biology, The University of Texas at Tyler, Tyler, TX, 1999

B.S., Biology, The University of Texas at Tyler, Tyler, TX, 1996

A.A., Science, Lon Morris College, Jacksonville, TX, 1992

High School Diploma, Jacksonville High School, Jacksonville, TX, 1990

EXPERIENCE

Clinical Preceptorship in Chemistry, UT Health Northeast, Tyler, TX, July 2016

Analyzed patient samples with Cobas 6000 and Cobas e411 automated analyzers, performed toxicology screens on urine samples with MedTox instrument, performed ANA/dsDNA screens, and performed IFE/SPE analyses.

Clinical Preceptorship in Microbiology, UT Health Northeast, Tyler, TX, July 2015

Worked with aerobic and anaerobic clinical isolates for proper identification with MicroScan Walkaway system and traditional methods, reported to physicians via LIS, identified mycological isolates to species level, performed rapid tests for influenza and occult blood

Laboratory Technical Services Supervisor, The University of Texas at Tyler, Tyler, TX, June 2006-Present

Upkeep of laboratory classrooms, ordering of supplies and maintenance of inventory.

Preparation of bacterial isolates and media for the Microbiology laboratory.

Department Safety Liaison for the Department of Biology.

Responsible for collection and safe disposal of biohazardous materials waste chemicals.

Ensure compliance with all state and federal safety regulations.

Research Associate, The University of Texas Health Center at Tyler, Tyler, TX, January 2005-June 2006

Key contributions:

Signal transduction assays in Protein Kinase C cascade

Signal transduction assays in MAP Kinase Cascade

Research Assistant, The University of Texas Health Center at Tyler, Tyler, TX, January 1999-January 2005

Key contributions:

Genetic studies in Mycobacterium spp

Signal transduction assays in urokinase plasminogen activator in lung epithelial cells

LABORATORY EXPERIENCE

I have experience in the following Microbiological and Molecular Biology techniques:

Clinical specimen processing and plate reading

Procaryotic cell culturing

Eucaryotic cell culturing (endothelial cells and epithelial cells)

SDS-PAGE electrophoresis of proteins and Western blotting

Native PAGE

Immunoprecipitation

RNA extraction

plasmid DNA extraction and restriction enzyme analysis

PCR

RT-PCR

gene cloning

Whole cell protein extraction

Nuclear and Cytosolic protein extraction

Adenovirus transfection of mammalian cells

RNAi

ELISA

signal transduction

protein induction

protein-mRNA interaction (Gel shift and UV Crosslink analyses)

Northwestern assay

ADDITIONAL COMMENTS:

I have had several years of experience in a research laboratory and I believe my experience would be useful in the laboratory. I also have had experience in keeping track of inventories and ordering supplies. If hired, I would be an effective member of the laboratory and would be able to perform my duties in a timely and efficient manner.

PROFESSIONAL REFERENCES

Ms. Maequeta Humber, Administrative Assistant II

The University of Texas at Tyler

Department of Biology

3900 University Boulevard, BEP 129A

Tyler, TX 75799

903-***-****

acv36p@r.postjobfree.com

Ms. Marla M. Stevenson, MT (ASCP)

Clinical Coordinator

UT Health Northeast

11937 US Hwy 271, BMR Room 314

Tyler, TX 75708-3154

903-***-****

acv36p@r.postjobfree.com

Dr. Hua Tang

UT Health Northeast

11937 US Highway 271

Tyler, TX 75708

903-***-****

acv36p@r.postjobfree.com

AWARDS, ACTIVITIES, and INTERESTS

STAR Employee of the Month, The University of Texas at Tyler, July 2008

Member, Phi Theta Kappa National Honor Society

Member, Alpha Chi National Honor Society

Member, Tyler Amateur Radio Club and East Texas Emergency Communications Service

Member, Association of Clinical Research Professionals

Member, UTMB Chapter of Alpha Eta Honor Society

Member, UTMB Beta Gamma Chapter of Lambda Tau Honor Society

Member, Association of Clinical Research Professionals

PRESENTATIONS AND PUBLICATIONS

Low, Brad D. and James R. Stewart. 1996. Preliminary Report of an Unknown Gram-Positive, Asporogenous Rod that Grows in Crystal Violet. Texas Branch ASM Convention (Junction, TX), March 21-23.

Low, Brad D. 1999. Identification of an Unknown Bacterium that Grows in Crystal Violet using PCR and Base Sequence Analysis. Master’s Thesis. The University of Texas at Tyler.

Yamamoto, Kohji, Brad Low, Stacey Rutherford, Malini Rajagopalan, and Murty V.V.S. Madiraju. 2001. The Mycobacterium avium-intracellulare Complex dnaB locus and Protein Intein Splicing. Biochemical and Biophysical Research Communications 280: 898-903.

Tang, Hua, Brad Low, Stacey Rutherford, and Qin Hao. 2005. Thrombin induces endocytosis of endoglin and type-II TGF- receptor and down-regulation of TGF- signaling in endothelial cells. Blood 105 (5): 1977-1985.

Tang, Hua, Qin Hao, Stacey A. Rutherford, Brad Low, and Z. Joe Zhao. 2005. Inactivation of Src family tyrosine kinases by reactive oxygen species in vivo. J. Biol. Chem., 280 (25): 239**-***** (Epub 25 Apr 2005).

Hao, Qin, Stacey A. Rutherford, Brad Low, and Hua Tang. 2006. Suppression of the Phosphorylation of Receptor Tyrosine Phosphatase-α on the Src-Independent Site Tyrosine 789 by Reactive Oxygen Species. Mol. Pharmacol. 69: 1938-1944.

Hao, Qin, Stacey A. Rutherford, Brad Low, and Hua Tang. 2006. Selective regulation of hydrogen peroxide signaling by receptor tyrosine phosphatase-α. Free Radical Biology & Medicine 41: 302-310.

Ge, Xiaona, Brad Low, Mei Liang, and Jian Fu. 2007. Angiotensin II Directly Triggers Endothelial Exocytosis via Protein Kinase C-Dependent Protein Kinase D2 Activation. J. Pharmacol. Sci. 105: 168-176.

Low, Brad, Mei Liang and Jian Fu. 2007. p38 Mitogen-Activated Protein Kinase Mediates Sidestream Cigarette Smoke-Induced Endothelial Permeability. J. Pharmacol. Sci. 104: 225-231.

I am acknowledged in the following publications (recognition of technical assistance):

Shetty, Sreerama, Usha R. Pendurthi, Prathap Kumar Shetty Halady, Ali O. Azghani, and Steven Idell. 2001. Urokinase induces its own expression in Beas2B lung epithelial cells. J. Biol. Chem. 276:245**-*****.

Greendyke, Rebecca, Malini Rajagopalan, Tanya Parish, and Murty V. V. S. Madiraju. 2002. Conditional expression of Mycobacterium smegmatis dnaA, an essential DNA replication gene. Microbiology 148: 3887- 3990.

Shetty, Sreerama, Khalil Bdeir, Douglas B. Cines, and Steven Idell. 2003. Induction of Plasminogen Activator Inhibitor-1 by Urokinase in Lung Epithelial Cells. J. Biol. Chem. 278 (20): 181**-*****.

Shetty, Sreerama and Steven Idell. 2004. Urokinase Receptor mRNA Stability Involves Tyrosine Phosphorylation in Lung Epithelial Cells. Am. J. Respir. Cell Mol. Biol. 30: 69-75.

Shetty, Sreerama, Harish Muniyappa, Prathap K. S. Halady, and Steven Idell. 2004. Regulation of Urokinase Receptor Expression by Phosphoglycerate Kinase. Am. J. Respir. Cell Mol. Biol. 31: 100-106.

Shetty. Sreerama, Thirunavukkarasu Velusamy, Steven Idell, Hua Tang, and Praveen Kumar Shetty. 2007. Regulation of urokinase receptor expression by protein tyrosine phosphatases. Am. J. Physiol. Lung Cell Mol. Physiol. 292: L414-L421.

Shetty, Sreerama, Thirunavukkarasu Velusamy, Steven Idell, Praveenkumar Shetty, Andrew P. Mazar, Yashodhar P. Bhandary, and Rashmi S. Shetty. 2007. Regulation of Urokinase Receptor Expression by p53: Novel Role in Stabilization of uPAR mRNA. Molecular and Cellular Biology 27 (16): 5607-5618.

Velusamy, Thirunavukkarasu, Praveenkumar Shetty, Yashodhar P. Bhandary, Ming-Cheh Liu, and Sreerama Shetty. 2008. Posttranscriptional Regulation of Urokinase Receptor Expression by Heterogeneous Nuclear Ribonuclear Protein C. Biochemistry 47: 6508-6517.

Shetty, Praveenkumar, Thirunavukkarasu Velusamy, Yashodhar P. Bhandary, Rashmi S. Shetty, Ming-Cheh Liu, and Sreerama Shetty. 2008. Urokinase Expression by Tumor Suppressor Protein p53: A Novel Role in mRNA Turnover. Am. J. Respir. Cell Mol. Biol. 39: 364-372.

Shetty, Sreerama, Yashodhar P. Bhandary, Shwetha K. Shetty, Thirunavukkarasu Velusamy, Praveenkumar Shetty, Khalil Bdeir, Margaret R. Gyetko, Douglas B. Cines, Steven Idell, Pierre F. Neuenschwander, Clemens Ruppert, Andreas Guenther, Edward Abraham, and Rashmi S. Shetty. 2010. Induction of Tissue Factor by Urokinase in Lung Epithelial Cells and in the Lungs. Am. J. Respir. Crit. Care Med. 181: 1355-1366.

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