Senior Lab Technologist

*** ******* ** ********** ** *****

910-***-**** ********@*****.***

SHANNON YORK

OBJECTIVE

I am searching for a position where I will be able to utilize my biology expertise, skills, and experience to obtain employment in a healthcare and/or laboratory setting

EDUCATION

August 2010- May 2012 NC A &T State University Greensboro, NC

Masters of Science in Biology

Advanced Certificate of Waste Management

August 2004- May 2009 NC A &T State University Greensboro, NC

Bachelor of Science in Biology

Certificate of Waste Management

August 1991- May 1993 Alamance Com. College Haw River, NC

Associate of Applied Science in Biotechnology

WORK EXPERIENCE

Laboratory Corporation of America Burlington, North Carolina October 2012- Present

Senior HLA Technologist/Lab Technologist

Perform tests and analyses according to established procedures in designated technical department

Testing used to aid in screening donors for use in Bone Marrow Transplantation.

Perform serological, molecular and immunological procedures that include but are not limited to DNA extraction, PCR amplification, sequence based typing, probe hybridization/detection

Responsible for HLA Class II sample Pre-Prep, various extraction methods, amplification, spotting, probing, developing, phoretix/result entry

Transplant SBT bench, reading SBT electropherograms, result interpretations, and other lab duties as assigned

Next Generation Sequencing (NGS) : multiplex sequencing with DNA barcode tags to simultaneously sequence large numbers of samples (some instrument maintenance)

Immune Cell Function Assay: detects cell-mediated immunity (CMI) by measuring the concentration of ATP from CD4 cells following stimulation. This measurement is made using heparin anti-coagulated whole blood, an illuminometer, and luciferin/luciferase. The assay is used for the detection of cell-mediated immunity in an immunosuppressed population

Research and Development; TruSight HLA new development for NGS, CCR5d32 allele isolation and integration into NGS, validation of CCR5d32, help with competency and validation of new Sciclone set-up, KIR exon 4 and 5 isolation and integration into NGS

Perform quality control, daily equipment maintenance

Responsible for training others in various procedures and methods

Carolina Biological Supply Co. Burlington, North Carolina December 1999-December 2004

Lab Technician

Facilitated instructions and duties for multiple labs and employees

Customer technical support

Inventory control and maintenance of all chemical reagents

Maintenance and QC of bacterial and yeast cultures

Media preparation and dispensing

Enzymatic digests of Lambda and Plasmid DNA

Plasmid growth, purification and quantitation using gel electrophoresis

Flexcell International Corp. Hillsborough, North Carolina August 1996-December 1999

Technical Specialist

Responsible for organization, production and inventory of cell culture plates

Cell culture for research and development and QC

Assisted in product packaging and shipping

Answering phones, taking orders, invoicing, price quotations, labeling, filing, manual and product information packet development and distribution, maintained personnel records and employee attendance, and general inventory

Developed and maintained customer databases

Provided guidance and direction to employees, as needed

INTERNSHIP EXPERIENCE S

NC A&T State University Greensboro, North Carolina Summer 2007 and 2008

Research Assistant/Math Instructor for ASCES Summer Institute Program

Improve Students competence in science

Help students learn how to collect and analyze scientific data, research specific biological diseases, see how math is applied to research and learn about mathematical equations, and learn how to use STELLA modeling computer program

U.S. Army Engineer Research and Development Center Construction Engineering Research Laboratory, Environmental Processes Branch, CNE Champaign, Illinois

Summer 2009

Research Intern

Project: Nanosomes Containing Insect Bio-receptors for Explosive Detection

Assist with and perform laboratory based experimentation to support a research project which aims to utilize the high sensitivity and specificity of honeybee olfactory receptor proteins for explosive detection

Assist in cloning and expression of olfactory receptor genes such that these may be manipulated to create an artificial olfactory system

RESEARCH EXPERIENCE S

NC A&T State University Greensboro, North Carolina Fall 2009-May 2012

U.S. Army Engineer Research and Development Center Construction Engineering Research Laboratory, Environmental Processes Branch, CNE Champaign, Illinois

Master’s Research:

Project: Encapsulation of pesticide degrading enzymes in tri-block copolymer vesicles. CERL-24: Pollution Prevention Research. ‘Encapsulation of pesticide degrading enzymes in tri-block copolymer vesicles’. U.S. Army Corps. Of Engineers, $43,400 (Funded)

Initial Goal: To clone, functionally express, purify and test the catalytic activity of OPH, PTE and or OPAA or derived mutants. To produce purified and catalytically active OPH, PTE and/or OPAA proteins

Overarching Goal: To encapsulate organophosphorus hydrolase (OPH) / phosphotriesterase (PTE) and or organophosphorus acid anhydrolase (OPAA) in polymer vesicles such that these pesticide degrading enzymes remain stable and functional

NC A&T State University Greensboro, North Carolina Fall 2006- Spring 2008

Undergraduate Research:

BLEND scholar : Project: Haemophilus ducreyi genomics

DNA purification, design primers, PCR, PCR troubleshooting, OEPCR, created a deletion cassette with Haemophilus ducreyi DNA, create mutant strain of bacteria

HONORS/AWARDS/ACTIVITIES

The National Scholars Honor Society (Magna Cum Laude)

Graduate student of the year

Research grant funded

Biomathematical Learning Enhancement Network Scholar (BLEND)

Golden Key International Honor Society

Phi Kappa Phi

Minority Association of Pre-Medical Students (MAPS)

Beta Beta Beta Biology Honors

SKILLS

Exceptional listener and communicator who effectively conveys information verbally and in writing

Flexible team player who thrives in environments requiring ability to effectively prioritize and juggle multiple concurrent projects

Dependable and responsible contributor who is committed to excellence and success

Self-starter, able to quickly master and apply complex concepts

Passionate behavior, positive attitude, self-discipline, personal integrity and respect for leadership/management, and other staff

Ability to maintain effective working relationships

Exceptional interpersonal, cooperative, and leadership skills

Quality Assurance & Control

Ability to prioritize and remain focused

Proficient with Microsoft Word, Excel, PowerPoint

Knowledge of safety procedures including but not limited OSHA, CLIA, HIPAA, PHI and Universal/Standard Precautions

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