Quality Control Manager

Johnny Ezedi

Education

Bachelor of Science, Biology

Salem State University

Professional Experience

AstraZeneca Pharmaceuticals

Laboratory Technician

**/**** – 11/2015

Managed Media/Compound Laboratory gained exposure to recent Tecan machines and supplied all compounds for machine operation.

Worked with Principal Scientist Performing Protein Expression in E.coli.

Harvested and analyzed expressed Protein by SDS-Page and Western blot.

Prepared Media such as M9 media, LB, MHB 11, MHA, Tryptic Soy, RPMI, Solutions, Buffer preparation for (infection control), Cancer Bioscience, Vivarium, Cell culture, Aliquot FBS, GLUT, PEN/STREPS (cell culture), Autoclave, Special Media preparation, Western blot, PM maintenance of HPLC instrument, Solvents, and Hollow fiber instrument maintenance.

Responsible for I-buy software for purchasing. Attended to customer’s complaint, and meet customer’s demands.

Acted as Assistant Reagent Manager, responsible for compound management and for supplying various organic and inorganic compounds to the chemists.

Responsible for Scout database to locate and distribute incoming compounds. Wrote and edited protocols and quality control.

Performed DNA sample preparation and liquid handling with automated robotic instruments and sequencers.

Assisted DMPK group by weighing out compounds for solubility and Formulation Studies.

Worked closely with the cell Banking group, retrieving customer’s order from data base, removing cells from cryo freezers and package to various customers.

Prepared and completed the entire R&D special Media request, and precisely documented all media prep activities in a timely manner.

Assisted in maintenance and operation of Lab equipment, including autoclave, balance, pH meter, and Milli Q water daily Calibration.

Gensi Lab

Account Manager

02/2012 – 12/2013

Supply lab consumables to customer in the east coast, robotic tips, 384 deep well plates, and liquid handling tips.

Responsible for development of a territory and account specific sales plan

Executed plans to meet sales quotas.

Demonstrated role and relevance of Gensi’s product portfolio and provide consultation to customers on same.

Effectively generate new customer Leads, and maintain strong customer base.

Aline Bioscience

Production Scientist

08/2009- 01/2011

Executed DNA sequencing; manual and automated, and gel electrophoresis.

Carried out ALINE PCR reagent preparation for high throughput environment, test reagent, samples, barcode before release, check temperature, and find root cause of problems.

Performed High throughput screening, mammalian Cell culture, PCR precipitation, high throughput sequencing in the production lab, and quality control.

Responsible for Reagent Clean-ups: After the reaction is completed reagents from PCR, enzymes were isolated. Water treatment, prepare RODI water fort the liquid handling equipment.

Operated Beckman Coulter FX Instruments, Reconcile stocks with inventory, Tecan and liquid handling automation equipment, ship and package customer order, return customers samples, autoclave, bio hazard and chemical storage, BL1, BL2 laboratory safety in regulation with OSHA, universal safety precaution, clean room and maintenance of the lab area.

Agencourt Bioscience

Production Scientist

03/2003 – 07/2006

Responsible for reagent preparation including regular and large reagent preparations, clean sequence, AMPURE, media preps, SNP reagent preparation and sequencing, agars, and gel electrophoresis preparation.

Operated automated instruments such as Sequencer 384 well plates and Biomeck robotic instrument, automated colony picking and inoculation, liquid handling automation equipment, and sequencing.

Responsible for instrument maintenance and troubleshooting establishing equipment cleaning protocols and involved in regular maintenance.

Involved in used DNA plate organization and location maps, quality control management, testing samples and recorded with barcode compounds and store in deep freezer.

Genome Therapeutics Corporation, Waltham, MA

Production Scientist

1/2000 – 01/2003

Reagent Preparation: Preparation and sample loading on ABI mega base 1000 Sequencer, sequencing samples, precipitation, gel electrophoresis, gel analysis, PCR.

Operated automated instruments such as Sequencer 384 well plates, Q-bot robotic instrument, Tecan, liquid handling automation equipment.

Maintained and cleaned the Q-bot robotic instrument for colony picking, DNA analysis, Quality control management, inspect compound reagents for safety before releasing into production group, test and aliquot solution, Rodi water, barcode samples and entered into data base.

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