Applicant: Zizhao Liu acufx4@r.postjobfree.com

Education:

**/****-**/**** ***** Pharmaceutical University, Nanjing, China B.S. in Bioengineering, GPA 3.8/4.0

**/****-**/**** ********** of Arkansas, Fayetteville Ph.D. in Chemical Engineering, GPA 3.9/4.0

Research Experience

04/2013-Present Develop Responsive Adsorptive Membranes for High Efficiency Hydrophobic Interaction Chromatography (Advisor: X. Qian, Co-advisor: R. Wickramasinghe)

Developed responsive HIC membranes with high capacity (close to HIC phenyl membrane) and facile elution advantage using poly(vinylcaprolactam) as ligand

Investigated salts, proteins and pH effects on binding capacity and recovery

Developed protocol for cleaving ligands grafted by ATRP from RC membranes

Characterized responsive ligands by AFM, GPC, turbidity and dynamic light scattering

Designed comb-like and novel pH and thermo dual-responsive HIC ligands 08/2011-03/2013 Develop High Performance Affinity Membrane Adsorbers for Protein Separations (Advisor: X. Qian, Co-advisor: R. Wickramasinghe)

Self-synthesized tweezer-like bisphosphonate based (Arg) affinity monomer

Established ATRP grafting condition for copolymeric affinity ligands

Investigated copolymer chain length effects on lysozyme (Arg rich) static/dynamic binding

Illustrated the selectivity mechanism by monomer design (single/double arm) and combined with collaborator’s MD simulation results. Other Collaborated Projects

MAST center projects (with Southwestern Energy Company and Tyson): provided data for membrane surface characterization (SEM,AFM,XPS,ATR-FTIR, contact angle)

Provided dynamic light scattering data for lysozyme unfolding studies by simulations Expertise

Hands-on experience on protein purification with ligand design of membrane chromatography (membrane surface modification)

Hands-on experience on process development with AKTA FPLC (affinity and HIC),density gradient ultracentrifugation (virus) and filtration (depth filter and TFF)

Hands-on experience of several analytical methods: biomolecule/synthetic polymer characterization (dynamic light scattering, SDS-PAGE, GPC, nuclei acid electrophoresis), SEM, AFM (in air and in liquid), ATR-FTIR, contact angle and assays

(BCA protein assay and qPCR)

(Expect Date)

Hands-on experience with Planova virus membrane filtration process (trained by Asahi company)

Award & Professional Membership

Finalist in EMD Millipore's 2015 Life Science Award Program

2016 ACS BIOT Student Travel Award

ACS and AIChE member since 2013

Activity

Served as a session aide in 2013 AIChE Annual Meeting, San Francisco

Served as a teaching assistant for CHEG courses (Intro I, II, Chemical process safety, CHEG Lab I,III)

Advised two honors undergraduate research projects: “Investigating Lysozyme Unfolding and Aggregation in Salt Solutions Using Dynamic Light Scattering”,

“Design New Responsive Ligand for Membrane Based Hydrophobic Interaction Chromatography”

Reviewed 3 journal articles from Separation Science and Technology (IF 1.17) Publication

1. Liu, Z., Wickramasinghe, S. R., Qian, X. “An Overview of Membrane Chromatography: Ligand Design, Membrane Surface Modification and Characterization” in preparation, Separation Science and Technology, 2016

2. Liu, Z., Wickramasinghe, S. R., Qian, X. “The Effects of Salt Ions on Responsive Hydrophobic Interaction Membrane Chromatography” in preparation, Langmuir, 2016 3. Khang, A, Ravishankar, P, Krishnaswamy, A, Anderson, P, Cone, S, Liu, Z, Qian, X, Balachandran, K. “Engineering Anisotropic Biphasic Janus-type Polymer Nanofiber Networks via Centrifugal Jet Spinning” in reivew, Nano Letters, 2016 4. Du, H., Liu, Z. Jennings, R., Qian, X. “The Effects of Salt Ions on the Dynamics and Thermodynamics of Lysozyme Unfolding” in reivew, Biophysical Journal, 2016 5. Liu, Z., Du, H., Wickramasinghe, S. R., Qian, X. “Membrane Surface Engineering for Protein Separations: Experiments and Simulations”, Langmuir, 2014, 30 (35), pp 10651– 10660

Selected Presentation

1. Liu, Z., Wickramasinghe, S. R., Qian, X. “Developing high performance responsive HIC membranes”, oral presentation of 250th ACS National Meeting, San Diego, CA, 2016 2. Liu, Z., Wickramasinghe, S. R., Qian, X. “Understanding the Effect of Salt Ions on Protein Adsorption in Novel Responsive Hydrophobic Interaction Membrane Chromatography”, oral presentation of 249th ACS National Meeting, Denver CO, 2015 3. Liu, Z., Wickramasinghe, S. R., Qian, X. “The Effect of Salt Ions on Responsive Membrane Based Hydrophobic Interaction Chromatography” oral presentation of AIChE Annual Meeting, Atlanta GA, 2014

4. Liu, Z., Du, H., Wickramasinghe, S. R., Qian, X. “High Affinity Membrane Adsorbers for Protein Purification”, oral presentation of AIChE Annual Meeting, San Francisco CA, 2013 5. Liu, Z., Du, H., Wickramasinghe, S. R., Qian, X. “Developing Synthetic High Affinity Membrane Adsorbers for Protein Separations, oral presentation of 245th ACS National Meeting, New Orleans LA, 2013

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