Andrei Tomashevski, Ph.D.

***** ** ******** ** #***

San Antonio, TX 78231

Immigration status: Green card holder ( permanent resident)

Phone: 434-***-****

E-mail: acuccd@r.postjobfree.com

Skype: taj54taj

OBJECTIVES: Scientist / Senior Position in Biotech Company

SUMMARY:

Strong expertise design and implement immunization strategies for generation of poly- and monoclonal antibody.

Strong expertise in protein expression (bacteria, insect and mammals cells) and purification.

Excellent experience developing downstream processes for purification of proteins, including tagged and non-tagged proteins.

Excellent experience in chromatography (anion/cation exchange, size exclusion, affinity, hydrophobic)

Expertise in tangential flow methods.

Demonstrated excellent skills analyzing/summarizing data and delivering in a timely manner.

Possesses strong interpersonal, communication, analytical and troubleshooting skills.

Familiar with GLP and GMP like protocol

TECHNICAL COMPETENCIES

Protein purification :

Protein expression (in E.coli, mammalian and insect cells) and adjustment grow conditions

cell-free protein expression

Protein purification, (RP-HPLC, FPLC, LPLC, Affinity Chromatography, Ion Exchange Chromatography, Size Exclusion Chromatography)

AKTA, Agilent, Bio-Rad chromatography platforms

Design the scheme and strategy of protein purification

Development and optimization of protein purification processes

Purification IgG, IgM from native serum,

cell culture media, ascites fluid.

Scaling-up, scaling-down purification processes

Hand made affinity resins

TFF system

Biochemical/Biophysical mehtods:

coupling proteins include antibody

to resin beads

modify protein such as biotinylation

generate proteins conjugate

screening of small molecules

In vitro kinase and phosphatase assays

NHEJ assay

In vitro ubiquitination assay

Identification proteins (SDS-PAGE, Native -PAGE)

DSBs repair assay

NER repair assay

CHIP assay

Immunoprecipitation

Western, Southern Blots

protein-protein conjugation

protein-ligand conjugation

UV/Vis spectrometry

SPR

Circular Dichroism (CD)

Isotopic labeling

NMR sample preparation

preparation of protein samples for crystallization

Cell biology:

Generate of poly- and monoclonal antibody

Design and implement immunization strategies

ELISA, RIA, double immunodiffusion

Growth hybridoma cell in flask, roller

Induction ascites in mouse for monoclonal IgG

produce

Immuno and cytochemistry

Cultivation of different kind of mammalian cell include primary cells

Cultivation of insect cells

Cultivation and growth optimization of E.coli

Transient transfection of mammalian cells

Produce and maintenance stable cell lines

Screening compound libraries

Cytotoxicity assay

Flow cytometry

LAL assay

Molecular biology: Molecular cloning, site-direct mutagenesis,

sequencing, PCR, Phage display

Computer skills: Operating Systems: Windows, Linux

EDUCATION

Ph.D. in Biochemistry

Institute of Biochemistry and Physiology of

Microorganisms, Russian Academy of Sciences (Puschino, Russia)

Ph.D. Thesis: Purification and physic-chemicals properties of human

alfa-fetoprotein

M.S. in molecular biology

Institute of Biochemistry and Physiology of Microorganisms,

Russian Academy of Sciences (Puschino, Russia)

B.S. in cell biology and cytology

Leningrad State University (Leningrad, Russia)

Kuibyshev State University (Kuibyshev, Russia)

PROFESSIONAL EXPERIENCE

June 2014- now INTERNET consulting protein purification.

May 2010 -May 2014

Postdoctoral Fellow: Laboratory of Dr. D. Ivanov, Dept. Biochemistry UTHSCSA.

Project : Structural study of SPRY-domain from TRIM-5- alfa human and rhesus protein.

August 2008-August 2009

Postdoctoral Research Associate, Laboratory of Dr. Inna Kruman Garrison, Institute of Aging TTU.

Project: Cell cycle kinase activation is necessary for double DNA damage repair in neurons cell.

2007-July 2008

Postdoctoral Research Associate, Laboratory of Dr. Gary M. Kupfer, Department of Pediatrics, Section Hematology/Oncology, Yale University.

Project: Purification and study of biochemical properties of Fanconi anemia core protein FANCD2 complex

2002- 2007

Postdoctoral Research Associate, Laboratory of Dr. Gary M. Kupfer, Department of Microbiology, University of Virginia.

Project: Purification and study of biochemical properties of Fanconi anemia core protein complex

1999-2002

Postdoctoral Research Associate, laboratory of Dr. Inez Vincent, Department of Pathology, University of Washington, Seattle, WA

Project: Role of mitotic kinase (as like cdc2 ) in developing Alzheimer disease.

SELECTED PUBLICATIONS

1. X-Lding, J.Husseman, A. Tomashevski, D. Nochlin, L-W. Jin, I.Vincent

"The cell cycle cdc25A tyrosin phosphotase is activated in degenerating postmitotic neurons in Alzheimer disease."

Amer. J. Pathology 2000 157 (6) 1983-1990

2. Tomashevski A., Husseman J.W., Ding X-L., Zheng J-H., Vincent I.

"Constitutive Wee1 and Cdc25B activities in adult brain neurons with M phase-type alterations in Alzheimer neurodegradation. "

J. Azlheimer disease 2001 3(2) 195-208

3. Vincent I, Bu B., Tomashevski A.,Hudson K.

"Constitutive Cdc25B tyrosine phosphotase activity in adult brain neurons with M phase-type alterations in Alzheimer neurodegradation. "

J. Neurochem. 200*-***-***-***

4. Thomashevski A., High A.A., Drozd M., Shabanowitz J., Hunt D.F., Grant P.A., Kupfer G.M. The Fanconi anemia core complex forms four complexes of different sizes in different subcellular compartments.”

J. Biol. Chem. 2004 279(25) 262**-*****

5. Collins N.B., Wilson J.B., Bush T., Thomashevski A., Roberts K.J., Jones N.J., Kupfer G.M. ATR-dependent phosphorylation of FANCA on serine 1449 after DNA damage is important for FA pathway function

Blood 2009 113(10) 21-81-2190

6. Tomashevski A., Webster D., Grammas P., Gorospe M., Kruman I.

Cyclin C-dependent cell cycle entry is required for activation of nonhomologous end joining DNA repair in postmitotic neurons.

Cell death and differentiation 2010 17(7) 1189-1198

7. Williams S.A., Wilson J.B., Clark A.P., Mitson-Salazar A., Tomashevski A., Ananth S., Glazer P.M., Semmes O.J., Bale A.E., Jones N.J., Kupfer G.M.

Functional and physical interaction between the mismatch repair and FA-BRCA pathways.

Human molecular genetics 2011 20-22-439*-****

8. Biris N, Yang Y, Taylor AB, Tomashevski A, Guo M, Hart PJ, Diaz-Griffero FB, Ivanov DN.

Structure of the rhesus monkey TRIM5α PRYSPRY domain, the HIV capsid recognition module.

Proc. Natl. Acad. Sci. USA 2012 109(33) 3278-3283

9. Biris N., Tomashevski A., Bhattacharya A., Diaz-Griffero F., Ivanov D.

Rhesus monkey TRIM5 -alfa SPRY domain recognizes multiple epitopes that span several capsid monomers on the surface of the HIV-1 mature viral core.

J Mol Biol. 2013 425(24) 5032-44

Awards

1998-1999 The George Soros International Science Foundation Award

References

Available by request.

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