Miles C. Cowart

* ******* ***** • Hockessin, DE *9707 • 302-***-**** • actyyp@r.postjobfree.com

SUMMARY

-Versatile and reliable with proven analytical skills.

-In depth command of various molecular biology techniques.

-Experience with protein biochemistry and purification.

-Experience with mammalian cell culture and aseptic techniques.

-Equally capable of working as part of a team or independently.

SKILLS

Pharmacology - ligand binding assays with insecticidal proteins.

Proteomics - proteolytic processing and purification of insecticidal proteins. Molecular Biology - PCR, vector construction, and subcloning.

Cell Biology & Immunology - tissue dissection, cellular/sub-cellular fractionation, immunolabeling, Western blotting, immunoprecipitation,

ELISA, cell culture, cell transfection, cell function assays, confocal and conventional microscopy.

Electrophysiology - short circuit current measurements on isolated insect digestive tissue

Bioinformatics - nucleic acid and protein sequence analysis.

Computer skills and software - pClamp data acquisition and analysis, Unicorn, Originlab, Totallab, Phoretix. Proficient in MS Word, Excel, and PowerPoint.

EDUCATION

MS Biological Sciences, University of Delaware (2007) Advisor: Dr. Ulhas Naik

Thesis: Exploring the Role of NIPSNAP4, CIB1, and Plk3, in Megakaryocyte Maturation.

BA Biological Sciences, University of Delaware (2003)

EXPERIENCE

Senior Research Associate (2011 – present)

DuPont Pioneer, Wilmington, DE

-Characterized the mode of action of insecticidal proteins for the purpose of transgenic insect control and resistance management.

-Experimental design and data analysis.

-Group lead for Research Management System related to ISO9001 status.

-SOP development and review.

-Drafted project reports.

-Trained new personnel.

Research Associate (2007 - 2011)

DuPont Pioneer, Wilmington, DE

-Performed mode of action and site of action analysis of insecticidal proteins.

Research Assistant (2004 – 2006)

University of Delaware, Dept. of Biological Sciences, Laboratory of Cardiovascular and Cancer Biology, Newark, DE

-Identified a novel mitochondrial protein with a probable function in apoptosis and the maturation of megakaryocytes, the progenitors of platelets.

Teaching Assistant (2003-2004)

University of Delaware, Dept. of Biological Sciences, Newark DE

-Taught the laboratory portion of an introductory biology course and provided clerical support for the classroom professor.

Scientific Intern (2002)

Fraunhofer USA, Newark DE

-Assisted in the development of a procedure for the production of a hepatitis C vaccine in tobacco plants using the tobacco mosaic virus.

PUBLICATIONS

Zhao J-Z, Oneal MA, Richtman NM, Thompson SD, Cowart MC, Nelson ME, Pan Z, Alves AP, and Yamamoto T. Laboratory-Selected Western Corn Rootworm (Coleoptera: Chrysomelidae) Colony Resistant to mCry3A. J. Econ Entomology. [under review]

Contact this candidate