Professional Experience Medical

Zeenathual Mohamed Hussain

EDUCATION

Master of Science in Biotechnology

Bachelor of Science in Microbiology

CERTIFICATION & TRAINING

Clinical Research Associate-In-house, Q-tech solutions, New Jersey, USA

Certification of Mastery in Biology, MITx (edx.org), USA

Certification in Immunology, University of Rice (edx.org), USA

Certification in Genomics medicine gets personalized, Georgetown University (edx.org), USA

Certification in Medical Laboratory Technology (MLT)

TECHNICAL SKILLS

In vitro Cell Culture and Molecular Biology:

Experience with aseptic methods of mammalian cell culture and able to perform a wide range of research experiments such as cell count, RNA extraction, cDNA synthesis, qRT-PCR, Western Blot, Mitotracker, colony assay, MTT assay.

Immunocytochemistry, Agarose Gel Electrophoresis, Spectrophotometer, ELISA, FACS, Co-immunoprecipitaion

Familiar with cloning, Restriction Enzyme digestion and E. Coli Transformation, RFLP, RAPD

Cytometry skills

Developed and created assays for cytometry, analysed and interpreted data for biological meanings.

Performance of flow cytometric DNA cell cycle analysis in melanoma and normal cell lines to study the effects of radiation on cell proliferation.

Microbiology Laboratory Techniques:

Basic Microbiology techniques such as Gram staining, Media preparation, Dilutions & Inoculation, Plating, Identification and Enumeration of microorganisms.

Proficient in Antimicrobial efficacy and preservative efficacy testing.

Prepared, dispensed, autoclaved media in the laboratory.

Worked with bacterial and fungal cultures in aseptic conditions.

Hands-on experience with microscope (confocal and fluorescence), colony counter and other instruments used for microbiological sterilization and disinfection.

Documented laboratory analysis in accordance with GLP and SOP requirements.

PROFESSIONAL EXPERIENCE

Hackensack University Medical Center, Hackensack, NJ Nov 2014 – Present

Scientific Researcher@ Genomics & Biomarker Lab

Studied the effects of mitochondrial inhibition in combination with radiation to enhance the radio sensitivity and apoptotic rates in melanoma cells while preserving the survival of primary skin cells.

Involved in analyzing different molecular pathways in radiation resistant melanoma cells by using qRT-PCR and gene expression techniques.

Part of this research also cover protein expression analysis including Western blotting, ICC and FACS.

Results of this research will give higher impact in melanoma therapy by finding out a way to increase radio sensitivity of cancerous cells and at the same time reducing radio toxicity to normal tissue surrounding the cancerous lesions.

PUBLICATIONS

A combination of high dose rate (10X FFF /2400MU/min/10MV x-rays) and total low dose (0.5Gy) induces a higher rate of apoptosis in melanoma cells in vitro and superior preservation of normal melanocytes.

Sreeja Sarojini, Andrew Pecora, Natasha Milinovikj, Joseph Barbiere, Saakshi Gupta, Zeenathual M. Hussain, Mehmet Tuna, Jennifer Jiang, Laura Adrianzen, Jaewook Jun, Laurice Catello, Diana Sanchez, Neha Agarwal, Stephanie Jeong, Youngjin Jin, Remache Yvonne, Andre Goy, Alois Ndlovu, Anthony Ingenito, K. Stephen Suh.

Co-administration of paclitaxel with 2400MU/min (10xFFF, 10MV x-rays) at a low total dose 0.5Gy eliminates melanoma cells in vitro after 4 radiations and preserves normal skin cells.

Sreeja Sarojini, Mehmet Tuna, Saakshi Gupta, Diana Sanchez, Zeenathual Mohamed Hussain, Laura Adrianzen, Jennifer Jiang, Neha Agarwal, Christopher Tavares, Natasha Milinovikj, Laurice Catello, Yvonne Remache, Andre Goy, K. Stephen Suh* (On the process of submission)

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