Medical School

IGOR BAGAYEV

** ********, # *** 781-***-****

Malden, MA 02148 ***********@*****.***

Objective: Research Technologist/Technician in a Microscopy Research Core Facility

Skills:

-Scanning confocal microscopy (Zeiss, Olympus, Nikon, Bio-Rad and Leica systems), laser capture microscopy, light and fluorescence microscopy, live cells imaging.

-Imaging software Zeiss LSCM, Nikon EZ-C1, Nikon NIS Elements, Zeiss Image Browser, ImageJ, Volosity, MMI CellCut.

-Reagent preparation, mammalian cell culture, histology (harvesting, trimming, embedding, microtomy, H&E staining and others, coverslipping), molecular biology (gel electrophoresis, PCR), cytogenetic (FISH), flow cytometry.

-MS Office, MS Windows.

Experience:

2009-present Senior Research Technologist,

2002-2009 Research Technologist, Confocal Microscope Core, Neuroscience Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA

Provided state-of-the-art imaging microscopy technology for fixed and live cells and tissues along with scientific and technical expertise to assist Harvard Medical School researchers in experimental design and optimal image collection: training on all equipment, consultation about various imaging techniques, assistance with image analysis and troubleshooting microscope issues.

Reagent preparation, cell culture, histology, molecular biology, cytogenetic.

Assisted Harvard Medical School researches in preparation of scientific publications.

Performed calibration, care and preventive maintenance of LSCM (laser scanning confocal microscopes), LCM (laser capture microscope) and Epifluorescent microscope installed in the Core.

1997-2001 Instructor in Biology, Microscopy Core, Medical Institute, Dnepropetrovsk, Ukraine

Instructed graduate students in fluorescence and light microscopy.

Supervised undergraduate biology classes: reagent preparation, tissue and cell culture; flow cytometry; computer methods for statistical analysis of biological data.

Education: B.S. /M.S. in Biology, Graduated with Honors,

Dnepropetrovsk National University,

Dnepropetrovsk, Ukraine

Languages: English, Russian, Ukrainian

Citizenship: US citizen

References: Available upon request

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