Six Sigma Management
Resume:
JONATHAN H. NGUYEN
Johnston, IA 50131 **********@*****.***
CAREER SUMMARY
Accomplished research scientist with extensive expertise in plant tissue culture, culturing maize immature embryos and callus for transformation by micro projectile bombardment and Agrobacterium, with diverse experiences in mutator screens for root mutants, DNA isolation, minipreparation, genomic DNA isolation. Known for developing and deploying high-throughput T0 handling process to improve the T0 plants survival rate and reduce the cycle time for bioassay evaluation. Known for experiment design and implementation, quality control, initiating and leading collaborations across function groups. Reputation for accurate data analysis and interpretation. Effective in creating a positive and productive work environment.
PROFESSIONAL EXPERIENCE
DUPONT PIONEER, Johnston, IA 1999-2016
A large U.S. producer of hybrid seeds for agriculture. Dupont Pioneer is a major producer of genetically modified organisms, including genetically modified crops with insect and herbicide resistance.
Team Leader (Senior Research Associate- SRA) 2010-2016
Led and coordinated projects of gene testing and production, pipeline management for constructs throughput. Managed research associates, prepared goals, evaluated team members, and conducted year-end reviews. Coached and supported team members to achieve goals. Cultured maize immature embryos and callus for transformation by micro projectile bombardment and by Agrobacterium using a variety of genotypes. Prepared reagents, samples and standards according to SOPs. Maximized construct throughput to increase efficiency and minimize turn time. Recorded data pertaining to experimentation and presented scientific results at the meeting/conference. Disciplined to maintain scrupulous records according to LIMS directed workflow.
Successfully completed all requirements for Six Sigma Green Belt Certification. Improving Efficiency of T0 Handling Process. Led a team of ten members working together to come up with several methods to test. One of the methods showed the results that would increase the plants survival rate from 59% to 94% while reducing cycle time for bioassay from 38 days to 24 days. This method saves tremendous resources and is still used today.
Managed a team in a greenhouse improvement project to come up with a new transport system that is more efficient and ergonomic friendly. The new system transported two times more samples and eliminated physical lifting.
Assisted two team members to achieve LEAN Certifications by providing ideas and input.
Implemented a new model to the team that would reduce the surplus of productivity by 50%.
Received Monocot Transformation Department Service Excellence Award. This award is in recognition for outstanding contributions, dedication, and technical excellence to the success of the transformation production for Insect Control program.
Team was nominated for the Ag Biotech Productivity Award, as a member of the Plant Protection Transformation Team, which was created to honor and recognize significant contribution to the area of enabling technology.
Research Associate 1999-2010
Cultured maize immature embryos and callus for transformation by micro projectile bombardment and by Agrobacterium using variety of genotypes. Recorded data pertaining to experimentation, measured ingredients used in laboratory testing, prepared data summaries and reports, and prepared laboratory samples for analysis, following proper protocols.
Contributed to the commercial event production of the OptimumTM GATTM and Herculex R Extra.
Received a Crop Genetics Research and Development Contribution Award for contribution to achieving Vector construction; event generation/selection, and planning embryo production were completed to meet the timeline.
Jonathan H. Nguyen, 515-***-****, **********@*****.*** Page 2
MONSANTO, Chesterfield, MO 1998
Research Associate
Cultured maize immature embryos/callus for transformation by micro projectile bombardment and/or by Agrobacterium using variety of genotypes. Recorded data pertaining to experimentation, measured ingredients used in laboratory testing, prepared data summaries and reports, and prepared laboratory samples for analysis, following proper protocols.
PIONEER HI-BRED, Johnston, IA 1991-1998
Research Assistant
Responsibilities included planting, watering greenhouse plants, mutator screens for root mutants, planting “ragdolls” and scoring for abnormalities, DNA isolation, minipreparation, genomic DNA isolation, data collection, reporting, culturing maize immature embryos for transformation by micro projectile bombardment and/or by Agrobacterium, conducted transient assay.
Publication: Albertsen, M.C., A.M. Cigan, T.W. Fox, C.W. Garnaat, G.A. Huffman, Y. Li, J. Nguyen, D.D. Songstad and M. Trimnell. 1997. Production of male sterile maize plants by genetic transformation of an elite genotype. Agronomy Abstract p.151.
EDUCATION
Bachelor of Science, Microbiology, Iowa State University, Ames, IA
PROFESSIONAL TRAINING AND DEVELOPMENT
Antitrust, Appropriate Workplace Conduct Training for Managers, Business Ethics, Creating Diverse and Inclusive Culture, Crucial Conversation, Good Laboratory Practices (GLP), Laboratory Safety, Six Sigma, Reducing Sexual Harassment, Safe Driver, Social Media, Team Building, Time Management, Trait Purity Compliance
COMPUTER COMPETENCIES
Microsoft Office: Word, Excel, Power Point, Outlook, ISO9001
Laboratory Information Management System (LIMS)
CERTIFICATIONS / DESIGNATIONS
Six Sigma Green Belt
LANGUAGE
English, Vietnamese
VOLUNTEER EXPERIENCE
Habitat for Humanity, Meals from the Heartland, United Way, Safety Site Committee
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